Effects of shrink film wrapping, wax coating and paper packaging on the quality ‎attributes of pomegranates (Punica granatum L.) cvs. ‘Eshkafto’ and ‘Rabbab’ ‎during cold storage

Document Type : Full Paper


Assistant Professor, College of Agriculture and Natural Resources of Darab, Shiraz University, Darab, Iran


This study investigated the effects of six postharvest treatments including: 1) wrapping with shrink film, 2) wax coating, 3) paper packing, 4) wax coating plus film-wrapping, 5) wax coating plus paper packing, 6) uncovered, on the physicochemical quality attributes of pomegranate, cvs. ‘Eshkafto’ and ‘Rabbab’. Treated fruits stored at 3.5 °C and about 85 % relative humidity for up to 12 weeks. Mean weight loss of pomegranate cvs. ‘Eshkafto’ and ‘Rabbab’ was lower in film-wrapped (1.19%), wax coated plus paper packed (1.94%), wax coated (3.67%) and paper-packed fruits (4.27%) compared with uncovered fruits (9.61%), respectively. However, the efficiency of wax coating plus film-wrapping in reducing weight loss (1.01%) was not superior to film-wrapping alone (1.19%). Total phenolic content and antioxidant activity of film-wrapped and also wax coated plus film-wrapped fruits of cv. ‘Eshkafto’ significantly were higher than that, control fruit, but with cv. ‘Rabbab’ only total anthocyanin of these treatments were higher than control fruit. Film wrapping plus wax coating also was highly effective in reducing electrolyte leakage in the husk of fruits and might be enhanced low temperature tolerance in pomegranates. Cultivar ‘Eshkafto’ had the highest contents of total phenolic, total anthocyanin, total soluble solids, titratable acid, ethanol and antioxidant activity.


  1. Al-Mughrabi, M.A., Bacha, M.A. & Abdelrahman, A.O. (1995). Effect of storage temperature and duration on fruit quality of three pomegranate cultivars. Journal of the Saudi Society of Agricultural Science, 7, 239-248.
  2. Al-Said, F.A., Opara, L.U. & Al-Yahya, R.A. (2009). Physico-chemical and textural quality attributes of pomegranate cultivars (Punica granatum) grown in the Sultanate of Oman. Journal of Food Engineering, 90, 129-134.
  3. Aghdam, M.S. & Bodbodak, S. (2013). Physiological and biochemical mechanisms regulating chilling tolerance in fruits and vegetables under postharvest salicylates and jasmonates treatments. Scientia Horticulturae, 156, 73–85.
  4. R., Borochov-Neori. H. Tian, L. & Holland, L. (2019). The biodiversity of different traits of pomegranate fruit peels from a broad collection of diverse cultivars. A review: Scientia Horticulturae, 246, 842-848.
  5. Arabi, A., Barzegar, M., & Azizi, M.H. (2008). Effect of cultivar and cold storage of pomegranate juices on organic acid composition. ASEAN Food Journal, 15, 44-54.
  6. Barman, k. Asrey, R. & Pal, R.K. (2011). Putrescine and carnauba wax pretreatments alleviate chilling injury, enhance shelf life and preserve pomegranate fruit quality during cold storage. Scientia Horticulturae, 130, 795-800.
  7. Belay, Z.A., Caleb, O.J. & Opara, U.L. (2017). Impact of low and super-atmospheric oxygen concentration on quality attributes, phytonutrient content and volatile compounds of minimally processed pomegranate arils (cv. Wonderful). Postharvest Biology and Technology, 124, 119-127.
  8. Chater, J.M., Merhaut, D.J., Jia, Z., Mauk, P.A. & Preece, J.E. (2118). Fruit quality traits of ten California-grown pomegranate cultivars harvested over three months. Scientia Horticulturae, 237, 11-19.
  9. D'Aquino, S., Palma, A., Shirra, M., Continella, A., Tirbulato, E. & La Malfa, S. (2010). Influence of film wrapping and fludoxionil application on quality of pomegranate fruit. Postharvest Biology and Technology, 55, 121-128.
  10. Elyatem, S.M. & Kader, A.A. (1984). Post-harvest physiology and behavior of pomegranate fruits. Scientia Horticulturae, 24, 287-298.
  11. Fadavi, A., Barzegar, M.M., Azizi, H. & Bayat, M. (2005). Physicochemical composition of ten pomegranates (Punica granatum) grown in Iran. Food Science and Technology International, 11, 113-119.
  12. Fawole O.A. & Opara, L.U. (2014). Physicomechanical, phytochemical, volatile compounds and free radical scavenging properties of eight pomegranate cultivars and classification by principal component and cluster analyses. British Food Journal, 116,544-567.
  13. Fawole, O.A. Opara, U.L. (2013). Changes in physical properties, chemical and elemental composition and antioxidant capacity of pomegranate (cv. Ruby) fruit at five maturity stages. Scientia Horticulturae, 150, 37-46.
  14. Feyzi, F., Seifi, E., Varasteh, F., Hemmatt, K. & Fereydoni, H. (2018). The study of climatic conditions effect on physicochemical properties of pomegranate fruits cultivars Malas-e-Saveh and Malas-e-Yousef-Khani. Iranian Journal of Horticultural Science, 48, 833-843. (In Farsi).
  15. Ferriera da Mota, W., Chamhum Salomao, L.C., Cicon, P.R. & Finger, F.L. (2005). Waxes and plastic film relation to the shelf life of yellow passion fruit. Scientia Agricola, 60, 51- 57.
  16. Ghafir, S.A.M., Ibrahim, I.Z. & Zaid, S.A. (2010). Response of local variety ‘Shlefy’ pomegranate fruits to packaging and cold storage. Acta Horticulturae, 877, 427-432.
  17. Ghasemnezhad, M., Zareh, S., Rassa, M. & Sajadi, R.H. (2013). Effect of chitosan coating on maintenance of aril quality, microbial population and PPO activity of pomegranate (Punica granatum cv. ‘Tarom’) at cold storage temperature. Journal of the Science of Food and Agriculture, 93, 368-374.
  18. Gil, M. I., C. Garcia-Viguera, C., Aartés, F. & Tomás-Barberán, F.A. (1995). Changes in juice pomegranate pigmentation during ripening. Journal of the Science of Food and Agriculture, 68, 77-81.
  19. Hagenmaier, R.D. Shaw, P.E. (1992). Gas permeability of fruit wax coating. Journal of American Society for Horticulture Science, 117, 105-109.
  20. Koyama, S., Cobb, L.J., Mehta, H.H., Seeram, N.P., Heber, D., Pantuck, A.J. & Cohen, P. (2010). Pomegranate extract induced a poptosis in human cancer cells by modulation of the IGF-IGFBP axis. Growth Hormone & IGF Research, 20, 55-62.
  21. Kulkarni, A.P. & Aradhaya, S.M. (2005). Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93, 319- 323.
  22. Luengiwilia, K., Sukjamasia, K. & Kader, A.A. (2007). Responses of 'Clemenules Clementine' and ‘W. Murcott’ mandarins to low oxygen atmospheres. Postharvest Biology and Technology, 44, 48-54.
  23. Magwaza, L.S. & Opara, U.L. (2015). Analytical methods for determination of sugars and sweetness of horticulture products. A review. Scientia Horticulturae, 184, 179-192.
  24. Mahajan, P.V., Caleb, O.J., Singh, Z., Watkins, C.B. & Geyer, M. (2014). Postharvest treatments of fresh produce. Philosophical Transactions of the Royal Society A, 372, 1471- 2962.
  25. Matityahu, I., Marciano, P., Holland, D., Ben-Arie, R. & Amir, A. (2016). Differential effects of regular and controlled atmosphere storage on the quality of three cultivars of pomegranate (Punica granatum). Postharvest Biology and Technology, 115, 132-141.
  26. Meighani, H., Ghasemnezhad, M. & Bakhshi, D. (2014). Evaluation of biochemical composition and enzyme activities in browned arils of pomegranate fruits. International Journal of Horticultural Science and Technology 1, 53-65.
  27. Meighani, H., Ghasemnezhad, M. & Bakhshi, D. (2017). Effect of different postharvest coating on colour and anthocyanin content of pomegranate fruit cv. ‘Malas-e-Savehʼ during cold storage. Iranian Journal of Horticultural Science, 47, 833-843. (In Farsi).
  28. Mirdehghan, S. H., Rahemi, , Martinez-Romero, D., Guillen, F., Martiez-Romero, D. & Valero, D. (2007). Reduction of pomegranate chilling injury during storage after heat treatment: role of polyamines. Postharvest Biology and Technology, 44, 19-25.
  29. Mphahlelea, R.R., Fawolea, O.A. & Opara, U.L. (2016). Influence of packaging system and long-term storage on physiological attributes, biochemical quality, volatile composition and antioxidant properties of pomegranate fruit. Scientia Horticulturae, 211,140-151.
  30. Mphahlelea, R.R., Fawolea, O.A. Standerce, M.A. & Opara, U.L.  (2014a). Preharvest and postharvest factors influencing bioactive compounds of pomegranate (Punica granatum). A review. Scientia Horticulturae, 178, 114-123.
  31. Mphahlelea, R.R., Stander, M.A., Fawolea, O.A & Opara, U.L. (2014b). Effect of fruit maturity and growing location on the postharvest contents of flavonoids, phenolic acid, vitamin C and antioxidant activity of pomegranate juice (cv. Wonderful). Scientia Horticulturae, 179, 36-45.
  32. Nanda, S., Sudhakar Rao, D.V. & Krishnamurthy, S. (2001). Effects of shrink film wrapping and storage temperature on the shelf life and quality of pomegranate fruits cv. Ganesh. Postharvest Biology and Technology, 22, 61-69.
  33. O’Grady, L., Sigge, G., Caleb, O.J. & Opara, U.L. (2014). Bioactive compounds and quality attributes of pomegranate arils (Punica granatum) processed after long-term storage. Food Package Shelf life, 2, 30–37.
  34. Pala, C.U. & Toklucu, A.K. (2011). Effect of UV-C light on anthocyanin content and other quality parameters of pomegranate juice. Journal of Food Composition and Analysis, 6, 790-795.
  35. Palma, A., Schirra, M., D'Aquino, S., La Malfa, S. & Continella, G. (2009). Chemical properties change in pomegranate seeds packaged in polypropylene trays. Acta Horticulturae, 818, 323-330.
  36. Pareek, S., Valero, D. & Serrano, M. (2015). Postharvest biology and technology of pomegranate. A review: Journal of the Science of Food and Agriculture, 95, 2360-2379.
  37. Pesis, E., Aharoni, D., Aharon, Z., Ben-Arie, R., Aharoni, N. & Fuchs, Y. (2000). Modified atmosphere and modified humidity packaging alleviate chilling injury symptoms in mango fruit. Postharvest Biology and Technology, 19, 93-101.
  38. Pourcel, L., Routaboul, J.M., Cheynier, V., Lepiniec, L. & Debeaujon, I. (2007). Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends in Plant Science, 12, 29-36.
  39. Rastegari, H., Tehranifar, A., Nemati, S.H. & Fazifehshenas, M.R. (2014). Effect of pre-harvest application of salicylic acid on postharvest characteristics of pomegranate fruit and storage in cold store. Journal of Horticultural Science, 28, 360-368. (In Farsi).
  40. Safizadeh, M.R. (2019). Effect of active and passive modified atmosphere packaging on quality of pomegranate fruits cv. Rabbab Ney-Riz during cold storage. Iranian Journal of Horticultural Science and Technology, 20, 51-64. (In Farsi).
  41. Saftner, R.A. (1999). The potential of fruit coating and film treatments for improving the storage and shelf-life qualities of ‘Gala’ and ‘Golden delicious’ apples. Journal of American Society for Horticulture Science, 124, 682-689.
  42. Selcuk, N. & Erkan, M. (2014). Changes in antioxidant activity and postharvest quality of sweet pomegranates cv. Hicrannar under modified atmosphere packaging. Postharvest Biology and Technology, 92, 29-36.
  43. Selcuk, N. & Erkan, M. (2015). Changes in phenolic compounds and antioxidant activity of sour-sweet pomegranates cv. ‘Hicaznar’ during long- term storage under modified atmosphere packaging. Postharvest Biology and Technology, 109: 30-39.
  44. Sumbhate, S., Nayak, S., Goupale, D., Tiwari, A. & Gadon, R.S. (2012). Colorimetric method for the estimation of ethanol in alcoholic-drinks. Journal of Analytical Techniques, 1, 1-6.
  45. Tietel, Z., Bar, E., Lewinshon, E., Feldmesser, E., Fallik, E. & Porat, R. (2010). Effects of wax coatings and postharvest storage on sensory quality and aroma volatile composition of ‘Mor’ mandarins. Science of Food and Agriculture, 90, 995-1007.
  46. Valero, D. (2007). Reduction of pomegranate chilling injury during storage after heat treatment: role of polyamines. Postharvest Biology and Technology, 44, 19-25.
  47. Varasteh, F., Arzani, K., Barzgar, M. & Zamani, Z. (2012). Changes in anthocyanins in arils of chitosan-coated pomegranate (Punica granatum cv. Rabbab-e-Neyriz) fruit during cold storage. Food Chemistry, 130, 267-272.