Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Influence of methyl jasmonate and salicylic acid treatment on the antioxidant ‎enzyme changes, expression of‏ ‏bapt‏ ‏and biosynthesis of taxanes in in vitro plantlets ‎of hazelnut cv ‘Segorb’ ‎

Document Type : Full Paper

Authors
Rahimeh Hemmati Gougeh 1
Mohamadreza Fattahi Moghadam 2
Majid Shokrpour 3
Mohammadreza Naghavi 2
Alidad Varshochi 4
1 Ph.D. Candidate, College of Agriculture and Natural Resources, University of Tehran, ‎Karaj, Iran
2 Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Ph.D. Head of R&D, RANA Agro-Industry Corporation, Tehran, Iran
10.22059/ijhs.2020.292876.1739
Abstract
Taxol is a phytochemical medicine which is used as a chemothrapy medication to treat several cancers. The hazelnut has been suggested as alternative source of taxol and has gained much attention. In this study, the influence of two elicitors, methyl jasmonate and salicylic acid was applied on hazelnut cv. ‘Segorb’ plantlets originated from in vitro condition. The activation of antioxidant enzymes such as PAL, catalase, PPO, SOD as well as hydrogen peroxide content was studied. Also, this research was carried out intending to evaluate txs, dbat and bapt gene expression, biosynthesis and production of taxanes. The investigations of antioxidant enzyme activities and H2O2 content were indicated the significant effects of such elicitors on increasing H2O2 content and the subsequent rise in catalase activity. txs and dbat genes in hazelnut ‘Segorb’ cultivar did not express. The highest expression of bapt was observed during the second day of treatment with 0.1 mM of SA and 0.1 mM metyljasmonate, while the highest amount of taxanes detected was after treatment with metyljasmonate at 0.1mM, SA at 0.2 and 0.1 mM, receptively, where taxanes detection demonstrated an 8-fold increase compared to the control. Overall, the content of taxanes on the 2 th to 16th day was higher than control. The highest amount of 10-deacetyltaxol and taxol was detected on day 4 after elicitor application. In conclusion, two elicitors metyljasmonate and salicylic acid caused to increase relative gene expression of bapt.
Keywords
Anticancer phytochemical medicine
elicitor
gene expression
taxol
tissue culture
  1. Balestrasse, K., Gardey, L., Gallego, SM. & Tomaro, ML. (2001). Response of antioxidant defence system in soybean nodules and roots subjected to cadmium stress. Functional Plant Biology, 28(6), 497-504.
  2. Bemani, E., Ghanati, F., Yousefzadeg, B. & Khatami, F. (2012). Antioxidant activity, total phenolics and taxol contents response of hazel (Corylus avellana ) cells to benzoic acid and cinnamic acid. Notulae Botanicae Horti Agrobotanici, 40(1), 69-73.
  3. Bemani, E., Ghanati, F., Rezaei, A. & Jamshidi, M. (2013). Effect of phenylalanine on taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana) cells. Journal of Natural Medicines, 67, 446-451.
  4. Bestoso, F., Ottaggio, L., Armirotti, A., Baldi, A., Damonte, G., Degan, P., Mazzei, M., Cavalli, F., Ledda, B. & Miele, M. (2006). In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes. BMC Biotechnology, 6, 45.
  5. Chance, B. & Maehly, A.C. (1955) Assay of catalase and peroxidases. Methods in Enzymology. 11: 764-775.
  6. Driver, JA., Kuniyuki, AH. (1984). HortScience 19:507-509.
  7. Esmaeili, N., Ebrahimzadeh, H. & Abdi, K. (2017). Correlation between polyphenol oxidase (PPO) activity and total phenolic contents in Crocus sativus corms during dormancy and sprouting stages. Pharmacognosy Magazine, 13(3), 519-524.
  8. Fett-Neto, A.G., Pennington, J.J. & Di Cosmo, F. (1995). Effect of white light on taxol and baccatin III accumulation in cell cultures of Taxus cuspidata and Zucc. Journal of Plant Physiology, 146, 584-90.
  9. Gallego, A., Bonfill, M., Cusido, R. M., Pastor, M., Palazon, J. & Moyano E., (2015). Assessing factors that affect the growth of Corylus avellana cell suspension cultures: a statistical approach. In Vitro Cellular and Developmental Biology – Plant, 51, 530-538.
  10. Gallego, A., Imseng, N., Bonfill, M., Cusido, R.M., Palazon, J., Eibl, R. & Moyano, M. (2015). Development of a hazel cell culturebased paclitaxel and baccatin III production process on a benchtop scale. Journal of Biotechnology, 195, 93-102.
  11. Ghanati, F., Safari, M. & Hajnorouzi, A. (2015). Partial clarification of signaling pathway of taxanes increase biosynthesis by low intensity ultrasound treatment in hazel (Corylus avellana) cells. South African Journal of Botany, 96, 65-70.
  12. Global Paclitaxel Market Growth (2019-2024). From reportsweb.com.
  13. Han, M.W., Yoo J.K., Hong. D.N. 1997. Process of taxol from taxus cuspidate. S6066748A.
  14. Hemmati Gougeh, R., Fatahi Moghadam, M., Shokrpour, M., Naghavi, M. & Varshochi, A. (2017). Response of two hazelnut cvs Segorb and Round to plant tissue culture. Seconnd International and Tenth National Biotechonology Congress of Islamic Republic of Iran. Seed and Plant Improvement Institude, Karaj, Iran, PP: 1-5
  15. Hoffman, A., & Shahidi, F. (2009). Paclitaxel and other taxanes in hazelnut. Journal of Functional Foods, 1, 33-37.
  16. (2013). Micropropagation of Hazelnut (Corylus Species). A thesis presented to The University of Guelph. 1-18.
  17. Kang, H.M. & Saltveit, M.E. (2001). Activity of enzymatic antioxidant defense systems in chilled and heat shocked cucumber seedling radicles. Physiologia Plantarum, 113, 548-556.
  18. Kar, M. & Mishra, D. (1976). Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. Plant Physiology, 57, 315-319
  19. Lauchli, R. & Boland, W. (2003). Indanoyl amino acid conjugates: Tunable elicitors of plant secondary metabolism. The Chemical Record, 3, 12-21.
  20. Malik, S., Cusido, R.M., Mirjalili, M.H., Moyano, E., Palazon, J., Bonfill, M. (2011). Production of the anticancer drug taxol in Taxus baccata suspension cultures: A review. Process Biochemistry, 46, 23–34.
  21. Mejía-Teniente,, Durán-Flores, FD., Chapa-Oliver, AM., Torres-Pacheco, I., Cruz-Hernández, A., González-Chavira, MM., Ocampo-Velázquez, RV. & Guevara-González, RG. (2013). Oxidative and molecular responses in Capsicum annuum L. after hydrogen peroxide, salicylic acid and chitosan foliar applications. International Journal of Molecular Sciences, 14, 10178-10196.
  22. Mittler, R., Vanderauwera, S., Gollery, M. & Breusegem, V. (2004). Reactive oxygen gene network of plants. Trends in Plant Science, 9(10), 490-8.
  23. Mutlu, S., Karadagoglu, O., Atici, O., Nalbantoglu, B. (2013). Protective role of salicylic acid applied before cold stress on antioxidative system and protein patterns in barley apoplast. Biology Plantarum, 10535-013-0322-4.
  24. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant, 15, 473-497.
  25. Niyazazari, A., Chalavi, V. & Kabirnataj, S. (2019). Effect of salicylic acid and methyl jasmonate on production of phenol, flavonoid and pigments of Madagascar periwinkle (Catharanthus roseus) callus. Iranian Journal of Horticultural Science, 52, 1-10. (in Farsi).
  26. Onrubia, M., Moyano, E., Bonfill, M., Palazon, J., Goossens, A. & Cusido, R. (2011). The relationship between txs, dbat, bapt and dbtnbt gene expression and taxane production during the development of Taxus baccata Plant Science, 181, 282-287.
  27. Onrubia, M., Moyano, E., Bonfill, M., Cusido, R.M., Goossens, A. & Palazon, J. (2013). Coronatine, a more powerful elicitor for inducing taxane biosynthesis in Taxus media cell cultures than methyl jasmonate. Journal of Plant Physiology, 170, 211-219.
  28. Onrubia, M., Moyano. E., Bonfill, M., Exposito, O., Palazon, J. & Cusido, R.M. (2010). An approach to the molecular mechanism of methyl jasmonate and vanadyl sulphate elicitation in Taxus baccata cell cultures: the role of txs and bapt gene expression. Biotechnol Engineering Journal, 53, 104-111.
  29. Ottaggio, L., Bestoso, F., Armirotti, A., Balbi, A. (2008). Taxanes from shells and leaves of Corylus avellana. Journal of Natural Products, 71(1), 58-60
  30. Ramirez-Estrada, M., Vidal-Limon, H., Hidalgo, D., Moyano, E., Golenioswki, M., Cusidó, RM. & Palazon, J. (2016). Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules, 21(2), 182-206.
  31. Reed, B.M. (2013). Improving initiation and mineral nutrition for hazelnut (Corylus avellana) micropropagation. A Thesis submitted to Oregon State University, 1-123.
  32. Rezaei, A., Ghanati, F. & Behmanesh, M. (2011a). Increase Taxol production and relese by metyl jasmonate, ultrasound, and dibutylphatalate in hazelnut (Corylus avellana) cell culture. Iranian Journal of Plant Biology, 3(7), 55-71 (in Farsi).
  33. Rezaei, A., Ghanati, F., Behmanesh, M. & Mokhtari-dizaji, M. (2011b). Ultarsound-potentiated salicylic acid-induced physiological effects and production of taxol in hazelnut (Corylus avellana) cell culture. Ultrasound in Medicine and Biology, 37, 1938-1947.
  34. Rezaei, A., Ghanati, F., Behmanesh, M., Safari, M. & Sharafi, Y. (2013c). Synergistic accumulative effect of salicylic acid and dibutyl phthalate on paclitaxel production in Corylus avellana cell culture. Journal of Stress Physiology & Biochemistry, 9, 157-168
  35. Rezaei, A., Ghanati, F. & Behmanesh, M. (2011d). Statistic magnetic field improved salicylic acid effect on taxol production in suspension- cultured hazel (Corylus avellana) cells. From https: //www. Researchgate. net/publication/264878242
  36. Rezaei, A., Ghanati, F. & Behmanesh, M. (2011e). Methyl jasmonate-induced biosynthesis of taxol and expression of certain related genes by hazelnut (Corylus avellana) cells. Planta Medica, 77- 14.
  37. Shahi, A., Fatahi, MR., Zamani, Z. & Maali-Amiri, R. (2020). Study of physiological and biochemical responses of some hazelnut cultivars under drought stress and re-watering conditions. Iranian Journal of Horticultural Scienc, 51, 229-244. (in Farsi).
  38. Tasgin, E., Atici, O., Nalbantoglu, B., Popova, L.P.: Effects of salicylic acid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves. – Phytochemistry, 67, 710-715, 2006.
  39. The "Paclitaxel-market Analysis, Trends, and Forcasts". )2017). http://www. Research and market.com
  40. Velikova, V., Yordanov, I. & Edreva, A. (2000). Oxidative stress and some antioxidant systems in acid rain-treated been plants protective role of exogenous polyamines. Plant Science, 151, 59-66.
  41. Wang, JA., Zheng, LP., Wu, JY. & Tan, RX. (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide Biology and Chemistry, 15, 351-358.
  42. Zhao, J., Davis, LC. & verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 23, 283-333.
Iranian Journal of Horticultural Science
Volume 52, Issue 1
Spring 2021
Pages 225-242

  • Receive Date 05 December 2019
  • Revise Date 12 January 2020
  • Accept Date 24 February 2020