The Effect of Abscisic acid regulator on yield, antioxidant enzymes activity and ‎proline content of lavender (Lavendula angustifolia cv. Organic Munestead) in ‎response to deficit irrigation

Document Type : Full Paper

Authors

1 Ph. D. Candidate, Gorgan University of Agricultural Sciences and Natural Resources, ‎Gorgan, Iran

2 Assistant Professor, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Associate Professor, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 Assistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Abstract

Drought is one of the most important factors limiting plant growth worldwide and the most common environmental stress. In response to environmental stresses, abscisic acid (ABA) plays an important role in initiating and coordinating many physiological and biochemical processes in reducing oxidative stress. To study the effects of abscisic acid on some antioxidant enzymes and proline of Lavendula angustifolia cv. Organic Munestead under deficit irrigation stress, a pot experiment was conducted as factorial based on randomized complete block design with three replications. The treatments were four levels of irrigation regimes (30-40, 50-60, 70-80 and 90-100 percent field capacity (FC)) and ABA including three levels (0, 15 and 30 mM/L). Traits evaluated in flowering stage. The highest level of drought resulted an increasing 91% in proline and decreasing 29.88% in carotenoid compared to control. Abscisic acid had a significant effect on all traits, except of carotenoid content. Abscisic acid 30 mM/L spray and irrigation regimes 30-40 percent FC induced the highest content of CAT, POD and SOD, while the maximum of antioxidant activity (70.54 mg/g DW) has resulted in ABA 15 Mm/L application and irrigation regimes 50-60 percent FC and the maximum of proline (2.51 mg/g DW) has resulted in ABA 15 mM/L application and irrigation regimes 30-40 percent FC. The highest fresh and dry weight of shoot and root were obtained from non-application of ABS in irrigation of 90-100% FC. It is determined abscisic acid application in deficit irrigation stress conditions increased the activity of antioxidant enzymes of lavender to counteract the negative effects of deficit irrigation stress.

Keywords

References

  1. Arulbalachandran, D., Yasmin, K. & Jothimani, K., (2016). Role of ABA on antioxidant mechanism under drought crops. International Journal for Species, 17, 48-55.
  2. Ashraf, M.A., Rasheed, R., Hussain, I., Iqbal, M., Haider, M.Z., Parveen, S. & Sajid, M.A. (2015). Hydrogen peroxide modulates antioxidant system and nutrient relation in maize (Zea mays ) under water-deficit conditions. Archives of Agronomy and Soil Science, 61, 507-523. (in Farsi).
  3. Babaie, K., Amini Dehaghi, M., Modares Sanavie, S. & Jabarie, R. (2010). Effect of drought stress on morphological traits, proline content and thymol percentage in thyme (Thymus vulgaris). Iranian Journal of Medicinal and Aromatic Plant, 26, 251-259. (in Farsi).
  4. Bates, S., Waldern, R. P., & Teare, E. D. (1973). Rapid determination of free proline for water stress studies. Plant Soii, 39, 205-207.
  5. Baxter, A., Mittler, R., & Suzuki, N. (2014). ROS as key players in plant stress signaling. Journal of Experimental Botony, 65, 1229-1240.
  6. Brahmi, C., Kopp, C., Domart-Coulon, I., Stolarski, J. & Meibom, A. (2012). Skeletal growth dynamics linked to trace-element composition in the scleractinian coral Pocillopora damicornis. Geochimica et Cosmochimica Act, 99, 146-158.
  7. Chagas, R., Silveira, J., Ribeiro, R., Vitorello, V. & Carrer, H. (2018). Photochemical damage and comparative performance of superoxide dismutase and ascorbate peroxidase in sugarcane leaves exposed to paraquatinduced oxidative stress. Pestic. Comparative Biochemistry and Physiology, 90, 181-188.
  8. Choudhury, F., Rivero, R., Blumwald, E. & Mittler, R. (2017). Reactive oxygen species, abiotic stress and stress Combination. The Plant Journal, 90, 856-867.
  9. Dai, A. (2013). Increasing drought under global warming in observations and models. Nature Climate Change, 3(1), 52.
  10. Damalas, C. (2019). Improving drought tolerance in sweet basil (Ocimum basilicum) with salicylic acid. Scientia Horticulturae, 27, 360-365.
  11. Darvizheh, H., Zahedi, M., Abaszadeh, B. & Razmjo, J. (2019). Changes in some antioxidant enzymes and physiological indices of purple coneflower (Echinacea purpurea) in response to water deficit and foliar application of salicylic acid and spermine under field condition. Scientia Horticulturae, 247, 390–399.
  12. Du, B. & Rennenberg, H. (2018). Physiological responses of lavender (Lavandula angustifolia) to water deficit and recovery. Journal Science African of Botony, 119, 212–218.
  13. Foyer, C. & Noctor, G. (2003). Redox sensing and signaling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiologia Plantarum, 119, 355-364.
  14. Ghaderi, A.A., Fakheri, B.A. & Mahdi-nezhad, N. (2017). Evaluation of the morphological and physiological traits of thyme under water deficit stress and foliar application of ascorbic acid. Agricultural Crop Management, 19 (4), 817-835. (in Farsi).
  15. Ghassemi, S., Ghassemi-Golezani, K. & ZehtabSalmasi, S. (2019). Changes in antioxidant enzymes activities and physiological traits of ajowan in response to water stress and hormonal application. Scientia Horticulture, 246, 957–964.
  16. Giannopolitis, C.N. & Ries, S.K. (1977). Superoxide dismutase: occurrence in higher plants. Plant Physiology, 59, 309–
  17. Gorgini Shabankareh, H. & Khorasaninejad, S. (2017). The effects of Sodium nitroprusside on some physiological and biochemical properties of Satureja khuzestanica under irrigation regimes. Journal of Production Research, 3, 55-70. (in Farsi).
  18. Gorgini Shabankareh, H., Fakheri, B. & Mohammadpour vashvaie, R. (2017). The Effect of biofertilizer on growth, seed yield and fennel essential oil under water stress. Journal of Agroecology, 9(1), 50-62. (in Farsi).
  19. Gorgini Shabankareh, Khorasaninejad, S., Sadeghi, M. & Tabasi, A. (2018). The effect of irrigation interval and humic acid on morphophysiological and biochemical characteristics of thyme (Thymus vulgaris). Journal of Iranian Plant Ecophysiologycal Research, 51, 67-82. (in Farsi).
  20. Guler, N.S. & Pehlivan, N. (2016). Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine max) through inducing antioxidant system. Acta Biologica Hungarica, 67, 169-183.
  21. Herzog, V. & Fahimi, H.D. (1976). Intracellular distinction between peroxidase and catalase in exocrine cells of rat lacrimal gland: a biochemical and cytochemical study. Histochemistry, 46, 273–
  22. Hosseini Boldaji, S A., Khavari-Nejad, R A., Hassan Sajedi, R., Fahimi, H. & Saadatmand, S. (2012). Water availability effects on antioxidant enzyme activities lipid peroxidation, and reducing sugar contents of alfalfa (Medicago sativa ). Acta Physiologia Plantarum, 34, 1177-1186.
  23. Kafi, M., Borzoie, A., Salehie, M., Kamandi, A., Maasumie, B. & Nabati, J. (2009). Physiology of environmental stresses in plants. Academic Center for Education, 502.
  24. Khorasaninejad, S., Alizadeh Ahmadabadi, A. & Hemmati, K. (2018). The effect of humic acid on leaf morphophysiological and phytochemical properties of Echinacea purpurea under water deficit stress. Scientia Horticulturae, 239, 314–323.
  25. Kirecci, O. (2018). The Effects of Salt Stress, SNP, ABA, IAA and GA applications on antioxidant enzyme activities in Helianthus Annuus Fresenius Environmental Bulletin, 27, 3783- 3788.
  26. Kocsy, G., Tari, I., Vankova, R., Zechmann, B., Gulyas, Z., Poor, P. & Galiba, G. (2013). Redox control of plant growth and development. Plant Science, 211, 77-91.
  27. Kumar, S., Kaushal, N., Nayyar, H. & Gaur, P. (2012). Abscisic acid induces heat tolerance in chickpea (Cicer arietinum ) seedlings by facilitated accumulation of osmo protectants. Acta Physiologiae Plantarum, 34, 1651-1658.
  28. Li, L.J., Gu, W.R., Meng, Y., Wang, Y.L., Mu, J.Y., Li, J. & Wei, S. (2018). Physiological and biochemical mechanism of spermidine improving drought resistance in maize seedlings under drought stress. Ying Yong Sheng Tai XueBao, 29, 554-564.
  29. Lim, C.W., Baek, W., Jung, J., Kim, J. & Lee S.C. (2015). Function of ABA in stomatal defense against biotic and drought stresses. International Journal of Molecular Science, 16, 15251-15270.
  30. Lu, S., Sub, W., Li, H. & Guo, Z. (2009). Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2-and NO-induced antioxidant enzyme activities. Plant Physiology and Biochemistry, 47, 132-138.
  31. Maehly, A.C. & Chance, B. (1954). The assay of catalases and peroxidases. Methods of Biochemical Analysis. 1, 357-
  32. Mehrgan, B., Mosavifard, S. & Rezaienejad, A.H. (2018). The effect of potassium silicate foliar application on some physiological and biochemical morphological characteristics of Altranentra under drought stress. Journal of Crop Improvement, 20, 299-314. (in Farsi).
  33. Miliauskas, G., Venskutonis, P. R. & Van Beek, T. A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry, 85, 231-237.
  34. Moradi, P. (2017). Effect of drought stress on growth and hormonal changes of thyme (Thymus Vulgaris). Journal of Plant Process and Function, 6(19), 311-322. (in Farsi).
  35. Motamedi, H., Hemati, Kh. & Khorasaninejad, S. (2019). The Effect of abscisic acid foliar application on morphophysiological properties and some biochemical properties of Cannabis sativa under different soil conditions. Eco-phytochemical Journal of Medicinal Plants, 1, 12-24. (in Farsi).
  36. Mozafari, S., Khorasaninejad, S. & Gorgini Shabankareh, H. (2017). The effect of irrigation regimens on some physiological and biochemical characteristics of common purslane medicinal plant in greenhouse. Agricultural Crop Management, 19, 401-416. (in Farsi).
  37. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 22, 867–
  38. Namvar, A., Hadi, H. & Seyesdsharifi, R. (2017). The role of external sources of plant protection in modulating the destructive effects of non-biological tensions. Journal of Iranian Plant Ecophysiologycal Research, 12, 103-128. (in Farsi).
  39. Niknam, V., Razavi, N., Ebrahimzadeh, H. & Sharifizadeh, B. (2006). Effect of NaCl on biomass, protein and proline contents and antioxidant enzymes in seedlings and calli of two Trigonella Biologia Plantarum, 50, 591-5.
  40. Orang, S. & Davodnia, B. (2019). Evaluation of growth traits and secondary metabolites in Thymus vulgaris under mild stresses of salinity and drought. Eco-phytochemical Journal of Medicinal Plants, 6(2), 27-40. (in Farsi).
  41. Osakabe, Y., Yamaguchi-Shinozaki, K., Shinozak, K. & Tran, L. (2014). ABA control of plant macroelement membrane transport systems in response to water deficit and high salinity. New Phytologyst, 202, 35-49.
  42. Qi, J., Song, C.P., Wang, B., Zhou, J., Kangasjarvi, J., Zhu, J.K. & Gong, Z. (2018). Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack. Journal Integrative Plant Biology, 56, 1-23.
  43. Sah, S., Reddy, K. and Li, J. (2016). Abscisic asid and abiotic stress tolerance in crop plant. Frontiers in Plant Science, 7, 1-26.
  44. Sanjari Mijani, M., Sirousmehr, A.R. & Fakheri, B. (2015). The effect of drought stress and humic acid on some physiological characteristics of Hibiscus sabdariffa. Agricultural Crop Management, 17, 403-414. (in Farsi).
  45. Sharma, P., Jha, A., Dubey, R. & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 14, 1-26.
  46. Sodaie, H., Shamsaie, M., tajamolian, m., mibodi, A. & Hakimzadeh, M.A. (2016). The effect of drought stress on some morphological and physiological traits of savory. Plant Processes and Functions, 5(15), 1-12. (in Farsi).
  47. Stanev, S., Zagorcheva, T. & Atanassiov, I. (2016). Lavender cultivation in Bulgaria-21st century developments, breeding challenges and opportunities. Bulgarian Journal Biologicula Agricultural of Science, 22, 584-590.
  48. Sunoj, V.S.J., Naresh Kumar, S. & Muralikrishna, K.S. (2014). Effect of elevated CO2 and temperature on oxidative stress and antioxidant enzymes activity in coconut (Cocos nucifera) seedlings. Indian Journal of Plant Physiology, 19, 382-387.
  49. Swapna, S. & Shylaraj, K.S. )2017(. Screening for osmotic stress responses in rice varieties under drought condition. Journal of Rice Science, 24, 253-263.
  50. Taghipoure, Z., Asghari Zakaria, R., Zareh, N. & Shikhzade, P. (2014). The evaluation of some physiological traits in populations of Aegilops triuncialis under drought stress. Ranglands and Forests Plant Breeding and Research, 22, 55-66.
  51. Upson, T.M. & Andrews, S. (2004). The genus Lavandula, a botanical magazine monograph. Kew: Royal Botanical Gardens, Kew, GB,
  52. Wang, F., Liu, J., Chen, M., Zhou, L., Li, Z., Zhao, Q., Pan, G., Zaidi, S.-H.-R. & Cheng, F. (2016). Involvement of abscisic acid in PSII photodamage and D1 protein turnover for light-induced premature senescence of rice flag leaves. PLoS ONE, 11, 161-203.
  53. Yu, F., Wu, Y. & Xie, Q. (2016). Ubiquitin–proteasome system in ABA signaling: from perception to action. Molecular Plant, 9, 21-33.
Iranian Journal of Horticultural Science
Volume 52, Issue 1
June 2021
Pages 195-211

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History

  • Receive Date: 11 November 2019
  • Revise Date: 31 December 2019
  • Accept Date: 25 January 2020

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