Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effect of salicylic acid and methyl jasmonate on production of phenol, flavonoid and ‎pigments of Madagascar periwinkle (Catharanthus roseus L.) callus

Document Type : Full Paper

Authors
Atiyeh Niyazazari 1
Vida Chalavi 2
Sara Kabirnataj 1
1 M. Sc. Graduate, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Associate Profesosr, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
10.22059/ijhs.2019.263384.1496
Abstract
Salicylic acid (SA) and methyl jasmonate(MJ) are abiotic elicitors which can increase production of secondary metabolites in plants. Effects of SA and MJ on fresh weight, flavonoids, total phenol and pigments of Madagascar periwinkle callus were investigated in two factorial experiments, in a completely randomized design. The first factor was the concentration of a different abiotic elicitor, SA or MJ in 4 levels of 0, 50, 100 and 200 μM, and the second factor was time of using different concentrations of elicitors at 2 levels of 5 and 10 days, with three replications. Based on results, the interaction between time and SA in all traits, unless fresh weigh of callus and production of anthocyanin, was significant and the interaction between time and MJ on total phenol, chlorophyll and carotenoids production was significant (P<0.01). The highest total phenol content (55.123 mg/g F.W.) was obtained from 100 μM MJ treatment for 5 days and the highest amount of flavonoid (21.467 mg/g F.W.) was obtained in 50 μM SA treatment for 5 days. Therefore, 50 and 100 μM concentrations of SA and MJ abiotic elicitors in 5 days can be used for increase the production of flavonoid and total phenol in catharanthus roseus L. callus.
Keywords
Anthocyanin
carotenoids
‎‏ ‏chlorophyll
medicinal plant
  1. Abbaspour, J. & Ehsanpour, A. (2016). The impact of salicylic acid on some physiological responses of Artemisia aucheri Boiss. Under in vitro drought stress. Journal of Acta Agriculturae Slovenica, 107(2), 287-298.
  2. Babel, P., Devpura, V. & Purohit, S. (2014). Salicylic acid induced changes in growth and some biochemical characteristics in in vitro cultured shoots of chlorophytum borivilianum Sant. et Fernand. International Journal of Recent Scientific Research, 5(4), 774-779.
  3. Chaichana, N. & Srisulak, D. (2012). Effects of methyl jasmonate and salicylic acid on alkaloid production from in vitro culture of Stemona sp. International Journal of Bioscience, Biochemistry and Bioinformatics, 2(3), 146-150.
  4. Esmaeilzade, B. S. & Rezaei, N. A. (2013). Increased trigonelline production by salicylic acid in fenugreek (Trigonella foenum-graecum L.) cell culture. Journal of Cell & Tissue (JCT), 5(2), 165-172. (in Farsi).
  5. Fang, Y., Smith, M. & Pepin, M. (1999). Effects of exogenous methyl jasmonate in elicited anthocyanin-Producing cell cultures of ohelo (Vaccinium pahalae). Journal of Society for in Vitro Biology, 35(1), 106-113.
  6. Fukami, T. and Hildebrandt, A.C. (1967). Growth and chlorophyll formation in edible green plant callus tissues in vitro on media with limited sugar supplements. The Botanical Magazine Tokyo, 80(947), 199-211.
  7. Ghanati, F., Bakhtiyariyan, S. & Abdolmaleki, P. (2010). Effect of methyl jasmonate on secondary metabolites of Calendula officinalis L. Journal of Modarres Science & Biotechnic, 1(1), 21-33. (in Farsi).
  8. Goncalves, E., Cruz, R., Abreu, M., Brandao, T. & Silva, C. (2009). Biochemical and colour changes of watercress (Nasturtium officinale R. Br.) during freezing and frozen storsge. Journal of Food Engineering, 93(1), 32-39.
  9. Kepczynska, E. & Krol, P. (2012). The phytohormone methyl jasmonate as an activator of induced resistance against the necrotroph Alternaria porri f. sp. Solani in tomato plants. Journal of Plant Interactions, 7(4), 307-315.
  10. Keshavarz, R., Majid Mahdiyeh, M., Abnosi, M.H. & Amirjani, M.R. (2015). Effect of hydrogen peroxide on induction of secondary metabolites in periwinkle (Catharanthus roseus L.) callus. Journal of Cell & Tissue (JCT), 6(3), 389-396. (in Farsi). 
  11. Mehrabani, B., Nazeri, S. & Piri, K. (2012). Evaluation of total produced phenol in Chaei Koohi (Stachys lavandulifolia Vahi) callus culture and possibility of its enhancement using Elicitors. Journal of Agricultural Biotechnology, 4(2), 77-88. (in Farsi).
  12. Mishra, J.N. & Verma, N.K. (2017). A brief study on Catharanthus roseus: A review. International Journal of Research in Pharmacy and Pharmaceutical Sciences, 2(2), 20-23.
  13. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bio assays with Tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
  14. Pan, Q., Chen, Y., Wang, Q., Yuan, F., Xing, Sh., Tian, Y., Zhao, J., Sun, X. & Tang, K. (2010). Effect of plant growth regulators on the biosynthesis of vinblastine, vindolin and catharanthin in Catharanthus roseus. Journal of Plant Growth Regulators, 60, 133-141.
  15. Pirian, K. & Piri, Kh. (2012). Effect of methyl jasmonate and salicylic acid on noradrenalin accumulation in hairy roots of Portulaca oleracea L. International Research Journal of Applied and Basic Sciences, 3(1), 213-218.
  16. Rajabi, A., Abbaspour, H. & Sinki, J. M. (2016). Evaluation of chemical elicitors methyl jasmonate and salicylic acid effect on produce stimulation of hypericin in Perforatum hypericum. Journal of New Cellular & Molecular Biotechnology, 6(22), 41-50. (in Farsi).      
  17. Ram, M., Prasad, K., Singh, S., Hada, B. & Kumar, S. (2013). Influence of salicylic acid and methyl jasmonate elicitation on anthocyanin production in callus cultures of Rosa hybrid L. Journal of Plant Cell Tissue Organ Cult, 113(3), 459-467.
  18. Samadi, S., Ghasemnezhad, A., Alizadeh, M. & Alami, M. (2014). Influence of elicitors on photosynthesis pigments, caffeic acid, chlorogenic acid and proline content of Cynara scolymus callus Iranian Journal of Horticultural Science, 47(3), 435-443. (in Farsi).
  19. Singleton, V.L., Orthofer, R. & Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
  20. Talebi, M., Etesam, F. & Seyedtabatabaei, B. E. (2011). Indirect regeneration of Catharanthus roseus L. through leaf explants culture and different combination of plant growth regulators. Journal of Greenhouse Culture Science and Technology, 2(8), 35-44. (in Farsi).
  21. Tanouri, A., Ghasemnezhad, A. & Alizadeh, M. (2014). Effect of methyl jasmonate and salicylic acid on morphological traits and inside pigments in callus of Cynara scolymus. Journal of Crop Improvement, 16(4), 857-869. (in Farsi).
  22. Wagner, G. (1979). Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology, 64(1), 88-93.
  23. Wang, J., Qian, J., Yao, L. & Lu, Y. (2015). Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum. Bioresources and Bioprocessing, 2, 1-9.
  24. Zamani, S., Ghasemnezhad, A., Alizadeh, M. & Alami, M. (2015). Investigation on phenylalanine ammonia-lyase and phenylpropanoid combination activity of medicinal plant Cynara scolymus L. affected by salinity and salicylic acid in in-vitro conditions. Journal of Eco Phytochemistry of Medicinal Plants, (3)4, 28-39. (in Farsi).
  25. 25. Zamani, M., Moradi, H., Chalavi, V. & Kazemitabar, S. K. (2019). Effect of salicylic acid and methyle jasmonat elicitors on hypericin production in Hypericum perforatum L. cv. Topas callus culture. Iranian Journal of Horticultural Science, 49 (4), 915-923. (in Farsi).
Iranian Journal of Horticultural Science
Volume 52, Issue 1
Spring 2021
Pages 1-10

  • Receive Date 08 September 2019
  • Revise Date 29 November 2019
  • Accept Date 02 December 2019