Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effects of foliar application of nano iron oxide and biofertilizers on the activity of ‎antioxidant enzymes and some physiological characteristics of the root and aerial ‎parts in black cumin (Nigella sativa L.)‎

Document Type : Full Paper

Authors
Maryam Bromand Sivieri 1
Mostafa Heidary 2
Ahmad Gholami 2
Hadi Ghorbani 2
1 Ph.D. Candidate, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran‎
2 Associate Professor, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran‎
10.22059/ijhs.2019.274442.1591
Abstract
In order to study the effects of foliar application of nano iron oxide particles and biofertilizers on root growth, antioxidant enzymes activity in two parts of root and leaf, as well as some other physiological characteristics of black cumin (Nigella sativa L.) a pot experiment with factorial arrangement in randomized complete block design with three replications was carried out in 2017 growing season at the Faculty of Agriculture, University of Shahrood. The first factor was foliar applications of nano iron oxide at three levels (F0= control, F1= 1.5 and F2= 3 g /1 l water) and the second factor was biofertilizers at five levels (B0= Control, B1= Glomus Intraradices, B2= Glomus mosseae, B3= Azospirillum and B4= Azotobacter solution). The results showed that activity of antioxidant enzymes catalase, polyphenol oxidase in two parts of root and leaf was influenced by two factors of biological fertilizers and foliar application of nanoiron oxide particles, so that the use of biofertilizers reduced and with increasing iron oxide concentration, activity they increased. The highest activity of ascorbate peroxidase in leaf was obtained from F2B0 treatment. The interaction effect of foliar application of nanoiron oxide particles and biofertilizers had a significant effect on photosynthetic pigments and aerial dry weight, so that the highest amount of chlorophyll a was obtained from F2B1 treatment, chlorophyll b, aerial dry weight and iron of aerial from F2B2 treatment. According to the results of the experiment, the symbiosis with G. intrarardits fungus caused a significant increase in the absorption of nitrogen and iron elements in the plant.
Keywords
Ascorbate peroxidase
catalase
chlorophyll
mycorrhiza
polyphenol oxidase‎
  1. Arnon, A. N. )1967(. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121
  2. Asrar A. W. A. & Elhindi, K. M. (2011). Alleviation of drought stress of marigold (Tagetes erecta) plants by using arbuscular mycorrhizal fungi. Saudi Journal of Biological Sciences, 18, 93-98.
  3. Azcón, G., de Aguilar, C., Azcón, R. & Barea, J. M. )1979(. Endomycorrhizal fungi and Rhizobium as biological fertilisers for Medicago sativa in normal cuntivation. Natureresearch Journal 279: 325-236.
  4. Azimi, R., Jangju, M. & Asghari, H. R. (2014). Effects of mycorrhiza symbiosis on initial establishment and morphological traits of thyme (Thymus vulgaris) under natural conditions. Iranian Journal of Field Crops Research, 11(4), 666-676. (in Farsi).
  5. Bannister, J. V., Bannister, W. H. & Rotills, G. (1987). Aspects of the structure, function and application of superoxide dismutase. CRC Critical Reviews in Biochemistry, 22, 110-180.
  6. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
  7. Caris, C., Hordt, W., Hawkins, H. J., Romheld, V. & George, E. (1998). Studies of iron transport by arbuscular mycorrhizal hyphae from soil to peanut and sorghum plants. Mycorrhiza, 8, 35-39.
  8. Carlberg, I. & Mannervik, B. (1985). Glutathione reductase. Methods in Enzymology, 113, 484-490.
  9. Carmanol, F., Sinet, P. M., Rapin, J. & Jerome, H. (1981). Glutathione S-transferase of humen red blood cells assay, values in normal subjects and in two pathological circumstances: hyperbilirubinemia and impaired renal function. Clinica Chimica Acta, 117, 209-217.
  10. Chaudhary, V., Kapoor, R. & Bhatnagar, A. K. (2007). Effect of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia anmua L. Mycorrhiza, 17, 581-587p.
  11. Clive, L., Sze-Chung, S. & Nicholson, R. (1998). Reduction of light induced anthocyanin accumulation in inoculated sorghum mesocotyls implication for a compensatory role in the defense response. Plant Physiology, 116, 979-989.
  12. El-Fouly, M. M., Mobarak, Z. M. & Salama, Z. A. (2011). Micronutrients (Fe, Mn, Zn) foliar spray for increasing salinity tolerance in wheat Triticum aestivum L. African Journal of Plant Science, 5, 314-322.
  13. Esmaielpour, B. & Amani N. (2014). Investigating the effect of mycorrhizal inoculation on growth and uptake of nutrients in Lactuca sativa cv. Syaho. Journal of Soil Management and Sustainable Production, 4 (2), 49-68. (in Farsi).
  14. Eydizadeh K., Mahdavi Damghani A., Sabahi H. & Soufizadeh S. (2010). Effect of integrated application of biofertilizer and chemical fertilizer on growth of maize (Zea mays L.) in Shushtar. Journal of Agroecology, 2 (2), 292-301. (in Farsi).
  15. Hejazi, A. & Kafashi sedghi, M. (2014). Plant growth substances: principles and applications. Tehran University Press. 362pp. (in Farsi).
  16. Kar, M. & Mishra, D. (1976). Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology, 57, 315-319.
  17. Kaymak, H. C., Yarali, F., Guvence, I. & Figen Donmez, M. (2008). The effect of inoculation with plant growth rhizobacteria (PGPR) on root formation of mint (Mentha piperita L.) cuttings. African Journal of Biotecnology, 7(24), 4479-4483.
  18. Khan, A. G. (2005) Mycorrhizas and phytoremediation. In: Willey N. (Ed.): Method in Biotechnology-Phytoremediation: Methods and Reviews. Totowa, USA: Humana Press.
  19. Kokalis-Buerelle, N., Kloepper, J. W. & Reddy, M. S. (2006). Plant growth-promoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Journal of Applied Soil Ecology, 31, 91-100.
  20. Krizek, D. T., Britz, S. J. & Mirecki, R. M. (1998). Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cv. new red fire lettuce. Physiologia Plantarum, 103, 1-7.
  21. Kuckaki, A. & Banaian, M. (1994). Crop physiology. Mashhad University Press. 380 pp. (in Farsi).
  22. Mahmoudzadeh, M., Rasouli-Sadaghiani, M. H., Hassani, A. & Barin, M. (2015). The role of mycorrhizal inoculation on growth and essential oil of peppermint (Mentha piperita). Journal of Horticultural Science, 29(3), 342-348. (in Farsi)
  23. Marius, S., Octavita, A., Eugen, U. & Vlad, A. (2005). Study of a microbial inoculation on several biochemical indices in sunflower (Helianthus anuus L.). Genetics and Molecular Biology, 12 (2), 11-14.
  24. Marschner, H. (1995). Mineral nutrition of higher plants. (2nd ed.). New York: Academic Press, pp: 889.
  25. Marulanda, A., Porcel, R., Barea, J. M. & Azcon, R. (2007). Drought tolerance and antioxidant activities in launder plants colonized by native drought-tolerant of drought-sensitive Glomus species. Microbial Ecology, 54, 543-552.
  26. Mazaherinia, S., Astaraei, A, L., Fotovat, A. & Monsh, A. (2010). A comparison of uptake and concentration of iron (Fe) in wheat (Triticum aestivum L.) plant using ordinary and nano iron oxides. Agronomy Journal, 92, 103-111. (in Farsi)
  27. Mehraban, P. & Abdolzadeh, A. (2012). Effects of iron excess on the antioxidant activity and patterns of protein electrophoresis in Oryza sativa var. Shafagh. Journal of Plant Production, 19(1): 85-106. (in Farsi)
  28. Mita, S., Murano, N., Akaike, M. & Nakamura, K. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin that is inducible by sugars. Plant Journal, 11, 841-851.
  29. Mohammadi, M., Majnoun Hosseini, N. & Dashtaki, M. (2016). Effects of nano-ferric oxide and zinc sulfate on chlorophyll, anthocyanin, flavonoid and leaf mineral elements of peppermint (Mentha piperita L.) at Karaj climatic conditions. Iranian Journal of Medicinal and Aromatic Plants, 5(32), 770- 784. (in Farsi)
  30. Moneral, C. M., Derosa, M., Mallubhotla, S. C., Bindraban, P. S. & Dimkp, A. C. (2015). The application of nanotechnology for micronutrients in soil-plant systems. Virtual Fertilizer Research Center. VFRC Report. 44 pp.
  31. Nakano, Y. & Asada, K. (1987). Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiology, 28, 131-140.
  32. Perkin, E. (1982). Analytical methods for atomic absorption spectrophotometry. Perkin Elmer Corporation, Norwalk, Connecticut, USA.
  33. Peyvandi, M., Parande, H. & Mirza, M. (2011). Comparison on nano Fe chelate with Fe chelate on growth parameters and antioxidant enzymes active of Satureja hortensis. New Cellular and Molecular Biotechnology, 2(5), 25-32. (in Farsi)
  34. Porcel, R. & Ruiz-Lozano, J. M. (2004). Arbuscular mycorrhizal influence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress. Journal of Experimental Botany, 55, 1743-1750.
  35. Rafiei-Demneh, M., Shabani, L. & Sharifi Tehrani, M. (2014). Induction of antioxidant defensive mechanism in tall fescue inoculated with Glomus intraradices fungus under nickel stress. Journal of Plant Process and Function, 3(8), 35-46. (in Farsi)
  36. Rodony A. S. (2000). Plant nutrients. Acadmic press. Orlando, FL.
  37. Ruiz, J. M., Baghour, M. & Romers, L. (2000). Efficiencyof the different genotypes of tomato in relation to foliar content of Fe and the response of some bio indicators. Journal Plant Nutrition, 23, 1777-86.
  38. Samawati, S. & Malekoti, M. (2005). The necessity of using organic acids for quantitative and qualitative increase of agricultural products. Technical Publication No. 463. Senate Publications, Tehran, Iran. (in Farsi)
  39. Selvaraj, T. & Chellappan, P. (2006). Arbuscular mycorrhizae: A diverse personality. Journal of Central European Agriculture, 7, 349-358.
  40. Shady, M. A., Ibrahim, I. & Afify, A. H. (1984). Mobilization of elements and their effects on certain plant growth characteristics as influenced by some silicate bacteria. Egyptian Journal of Botany, 27(1-7), 17-30.
  41. Sharma, P. K. & Hall, D. O. (1991). Interaction of salt stress and photoinhibition on photosynthesis in barley and sorghum. Journal Plant Physiology, 138 (5), 614 - 19.
  42. Sinha, S. & Saxena, R. (2002). Effect of iron on lipid peroxidation, and enzymatic and nonenzymatic antioxidant content in medicinal plant Bacopa monnieril. Chemosphere, 22(3), 134-132.
  43. Stajkovic, O., Delic, D., Josic, D., Kuzmanovic, D., Rasulic, N. & Knezevic-Vukcevic, J. (2011). Improvement of common bean growth-promoting bacteria. Romanian Biotechnological Letters, 16(1), 5919-5926.
  44. Sun, B., Jing, Y., Chen, K., Song, L., Chen, F. & Zhang, L. (2007). Protective effect of nitric oxide on iron deficiency-induced oxidative stress in maize (Zea mays). Journal of Plant Physiology, 164, 536-543.
  45. Taherkhanchi, A., Akbari, Gh., Modares Sanavi, S. A. M. & Ghorbani Javid, M. (2012). Evaluation of effects of bio-fertilizers on some physiological and biochemical traits in soybean under water deficit condition. Agricultural Crop Management, 15(3), 141-153. (in Farsi)
  46. Vattani, H., Keshavarz, N. & Baghaei, N. (2012). Effect of sprayed soluble different levels of iron chelate nano fertilizer on nutrient uptake efficiency in two varieties of spinach (Varamin88 and Virofly). International Research Journal of Applied and Basic Sciences, 3(S), 2651-2656.
  47. Wahing. I, W., Van, V. J. G., Houba, J. & Vanderlee, J. (1989). Soil and plant analysis, a series of syllabi. part 7. plant analysis procedure. Wageningen Agriculture University.
  48. Yousef Zadeh, S., Naghdi Badi, H. A., Sabaghaniya, N. & Jan Mommadi, M. (2016). The effect of foliar application of nano-iron chelate on physiological and chemical traits of dragonhead (Dracocephalum moldavica L.). Journal of Medicinal Plants, 4 (60), 152-160. (in Farsi)
  49. Ziaee, T., Moharreri, N. & Hosseinzadeh, H. (2012). Review of pharmacological and toxicological effects of Nigella sativa and its active constituents. Journal of Medicinal Plants, 2(42), 16-42. (in Farsi)

 

Iranian Journal of Horticultural Science
Volume 51, Issue 4
Winter 2021
Pages 939-953

  • Receive Date 27 January 2019
  • Revise Date 20 May 2019
  • Accept Date 02 June 2019