Investigation the effect of humic acid on some morphhophysiological and biochemical ‎characteristics of almond rootstocks under salinity stress

Document Type : Full Paper

Authors

1 Ph.D. Candidate, Faculty of Agriculture, University of Mohaghegh‏ ‏Ardabili, Ardabil, Iran ‎

2 Associate Professor, Faculty of Agriculture, University of Mohaghegh‏ ‏Ardabili, Ardabil, Iran ‎

3 Associate Professor, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

Choosing suitable feed sources remove the problems of salinity from the plant. For this purpose, an experiment as factorial was conducted in a RCBD with three replications at the University of Mohaghegh Ardabili during 2015-16. The first factor was humic acid (HA) application at four levels: (0, 2.5, 5 and 7 kgha-1), the second factor was salinity at four levels: (0, 60, 120 and 180mM NaCl) and the third factor was two-years old almond rootstocks seedling at two levels (GF677 and GN15). Results showed that with increasing salinity, root/stem dry weight increased, especially in GF677, Na+ and Cl and SI increased, especially in GN15 and SLA, K+ and N, leaf osmotic potential, gs, Pn and T were decreased, especially in GN15. Carbohydrates increased to 120mM NaCl, especially in GF677 and then decreased. GF677 with an increase in HA up to 5 kgha-1, in the salinity of 180mM NaCl, with the highest root/stem dry weight and SLA, the lowest Na+ and Cl-and the highest K+and N of leaf had the highest leaf osmotic potential (-1.9MPa) and carbohydrate (86.92 mg gFW-1) compared to other rootstocks. The highest gs (0.53 mmol m-2s-1) and Pn (18.73 μmolCO2 m-2s-1) were found in GF677, 2.5 kgha-1 HA and 0 mM NaCl. With increasing HA, in these conditions, the SI decreased, especially in GF677. Therefore, GF677 was evaluated as the most tolerant rootstock for salinity and 2.5 and 5 kgha-1HA was evaluated as the most appropriate levels for affecting almond rootstocks.

Keywords


  1. Abbasi Siahjani, A., Yarnia M., Farahbakhsh, F. & Solar, M. (2017). Effect of rhizobium, pseudomonas and mycorrhizal fungi on red bean (Phaseolus vulgaris L.) traits under drought stress. Knowledge of Agriculture and Sustainable Production, 27(1), 85-102. (In Farsi)
  2. Al-Erwy, A. S., Al-Toukhy, A. & Bafeel, S. O. (2016). Effect of chemical, organic and bio fertilizers on photosynthetic pigments, carbohydrates and minerals of wheat (Triticum aestivum L.) irrigated with sea water. International Journal of Advanced Research in Biological Sciences, 3(2), 296-310.
  3. Annabi Milani, A., Neyshabouri, M., Mosaddeghi, M. & Zare Haqi, D. (2015). Relations between leaf water potential, stress-degree-day and water depletion in almond tree under salinity stress. Water and Soil Knowledge, 26(2/1), 189-206. (in Farsi)
  4. Asgharzade, A. & Babaeian, M. (2012). Investigation the effects of humic acid and acetic acid foliar application on yield and leaves nutrient content of grape (Vitis vinifera). African Journal of Micribiology Research, 6(31), 6049-6054.
  5. Ashraf, M. & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environment, Experimental Botany, 59, 206-216.
  6. Bahrami, V., Imani, A. & Piri, S. (2015). Evaluation of almond cultivars growth characteristics under salinity stress. Journal of Novel Applied Sciences, 4(12), 1227-1229.
  7. Balaket, R. T. M. A. & Al-Himidawi, S. A. M. (2014). Effect of humic acid and water quality on peroxidase and catalaze enzymes activity in leaves of date palms cv. Barhee. Global Journal of Bio Science and Biotechnology, 3(4), 402-405.
  8. Bayanloo, E., Aelaei, M. & Sani Khani, M. (2018). Effect of ϒ-aminobutyric acid (GABA), humic acid and salicylic acid on some morphophysiological responses and antioxidant characters of Catharanthusroseus L. (G.Don). Iranian Journal of Horticultural Science, 50(4), 993-1008. (in Farsi)
  9. Benhassaini, H., Fetati, A., Hocine, A. K. & Belkhodja, M. (2012). Effect of salt stress on growth and accumulation of proline and saluble sugars on plantlets of Pistacia atlantica Desf. Subsp. atlantica used as rootstocks. Biotechnology, Agronomy and Society and Environment, 16(2), 159-165.
  10. Bybordi, A. (2012). Study effect of salinity on some physiological and morphologic properties of two grape cultivars. Life Science Journal, 9(4), 1092-1101.
  11. Chartzoulakis, K., Loupassaki, M., Bertaki, M. & Androulakis, I. (2002). Effect of NaCl salinity on growth, ion content and Co2 assimilation rate of six olive cultivars. Scientia Horticulturae, 96, 235-247. 
  12. Cimrin, K. M., Turkmen, O., Turan, M. & Tuncer, B. (2010). Phosphorus and humic acid application alleviate salinity stress of pepper seedling. African Journal of Biotechnology, 9, 5845-5851.
  13. Duarte, H. H. F. & Souza, E. R. (2015). Soil water potential and Capsicum annum L. under salinity. Revista Brasileira de Ciencia do Solo, 40, 1-10.
  14. Farouk, S. (2011). Osmotic adjustment in wheat flag leaf in relation to flag leaf area and grain yield per plant. Journal of Stress Physiology and Biochemistry, 7(2), 117-138.
  15. Felipe, A. J. (2009). Felinem, Garnem and Monegro almond *peach hybrid rootstocks. Hort Science, 44(1), 196-197.
  16. Fischer, R. A., Rees, D., Sayre, K. D., Lu, Z. M. Condon, A. G. & Larque Saavedra, A. (1998). Wheat yield progress associated with higher stomatal conductance and photosynthesis rate and cooler canopies, Crop Science, 38(6), 1467-1475.
  17. Food and Agriculture Organization. (2017). Biodiversity: Agricultural biodiversity in FAO. Retrieved January 21, 2019, from istachio seedling under salinity stress. Journal of Nuts, 7(2), 125-135.
  18. Johnson, J. M. & Ulrich, A. (1975). Analytical meth. http://www.fao.org/biodiversity.
  19. Farhadi, H. Azizi, M. & Nemati, H. (2016). Investigation of the effects of salt stress on some physiological and biochemical characteristics of different landraces of fenugreek (Trigonella foenum - graecum L.). Intarnational Journal of Horticultural Science, 47(3), 531-541. (in Farsi)
  20. Hamada, A. M. & El-enany, A. E. (1994). Effect of NaCl salinity on growth, pigment, mineral contents and gas exchange of broad bean and pea plants. Biologia Plantarum, 36, 75-81.
  21. Hatami, E., Esna-Ashari, M. & Javadi, T. (2010). Effect of salinity on some gas exchange characteristics of grape (Vitis vinifera L.) cultivars. International Journal of Agriculture and Biology, 12(2), 308-310.
  22. Hosseini Chenarestanolya, M. Hosseini Frahi, M. & Aboutalebi, A. (2016). Effect of diffrent media culture and humic acid on some important vegetative properties of orange seedling cv. Valencia (Citrus sinensis) using sour orange (C. aurantium) rootstock. Intarnational Journal of Horticultural Science, 48(3), 487-502. (in Farsi)
  23. Irigoyen, J. J., Emerich, D. W. & Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of praline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Plant Physiology, 84, 55-60.
  24. Javanshah, A. & Aminian Nsab, S. (2016). The effects of humic acid and calcium on morpho-physiological traits and mineral nutrient uptake of p ods for use in plant analysis. Experiment Station, 766, 26-78.
  25. Mahmoudi, M., Samavat, S., Mostafavi, M., Khalighi, A. & Cherati, A. (2014). The effect of humic acid and proline on morphological properties of Actinidia deliciosa cv. Hayward under salinity. Journal of Applied Science and Agriculture, 9(1), 261-267.
  26. Mahmoudi, E., Shokouhian, A., Asghari, A. & Ghanbari, A. (2017). Study of the effect of humic acid application on quantitative and qualitative characteristics of kiwi Fruit cv. Hayward. Fruit Researches, 2(2), 108-96. (in Farsi)
  27. Mashahiri, Y. & Hassanpour Asil, M. (2018). Effects of gibberellic and humic acid on growth characters of doffodil (Narcissus jonquilla cv. German). Iranian Journal of Horticultural Science, 48(4), 875-886. (in Farsi)
  28. Meister, M. H. & Bolhar, H. R. (2003). Chapter 17: Stomata imprints: a new and quick method to count stomata and epidermis cells. In Reigosa roger, M. J. (Eds), Handbook of plant ecophysiology techniques, New York, Boston, Dordrecht, London, Moscow: Kluwer Academic Publishers, pp: 235-250.
  29. Parandian, F. & Samavat, S. (2012). Effects of fulvic acid and humic acid on anthocyanin, soluble sugar, α-amylase enzyme and some micronutrients in lilium. International Research Journal of Applied and Basic Sciences, 3(5), 924-929.
  30. Parida, A. K. & Das, A. B. (2005). Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 60(3), 324-349. 
  31. Radnia, H. & Babolghzadeh, A. (2012). Cultivars and rootstocks of fruit trees. Tehran, Applied Scientific Institute of Agricultural Jihad. (in Farsi)
  32. Saied, A. S., Keutgen, A. J. & Noga, G. (2005). The influence of NaCl salinity on growth, yield and fruit quality of strawberry cvs. Elsanta and Korona. Scientia Horticulturae, 103 (3), 289-303.
  33. Scholander, P. F., Hammel, H. T., Bradstreet, E. D., Hemingsen, E. A. (1965). Sap pressure in vascular plants. Science, 148, 339-346.
  34. Shafieizargar, A.R., Awang, Y., Ajamgard, F., Juraimi, A. Sh., Othman, R. & Kalantar Ahmadi, A. (2015). Assessing five citrus rootstocks for NaCl salinity tolerance using mineral concentrations, proline and relative water contents as indicators. Asian Journal of Plant Science, 14(1), 20-26.
  35. Zareiy, M., Azizi, M., Rahimi, M., Tehranian, F. A. & Davar Panah, S. (2017). Effect of salinity on some physiological and biological responses of four hybrid figs. Journal of Iranian Horticultural Science and Technology, 18(2), 143-158. (in Farsi)
  36. Zhang, L., Gao, M., Zhang, L., Li, B., Han, M., Kumar, A. & Ashraf, M. (2013). Role of exogenous glycinebetaine and humic acid in mitigating drought stress-induced adverse effects in Malus robusta seedlings. Turkish Journal of Botany, 37, 920-929.
  37. Zhang, J., Jia, W., Yang, J. & Ismail, A. M. (2006). Role of ABA integrating plant responses to drought and salt stresses. Field Crop Research, 97, 111-119.