The application of humic acid and nanocamposite superabsorbant on growth ‎characteristic and resistance to drought stress in turf grass

Document Type : Full Paper


1 Former M.Sc. Student, Faculty of Agriculture and Natural Resources, Arak University, ‎Arak, Iran

2 Associate Professor, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran

3 Assistant Professor, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran


In order to investigate the effect of nanocomposite superabsorbent and humic acid on the characteristics of lawn sport, a factorial experiment based on a completely randomized design with three replications was carried out. The first factor was nanocomposite superabsorbent at three levels (0, 2 and 5 g/Kg in each pot), the second factor was humic acid at three levels (0, 250 and 500 mg/L, as spray) and the thirs factor was drought stress at two levels (50 and 100% field capacity). Results showed that the application of humic acid during drought stress improved color traits and aerial part hieght. Application of nanocomposite superabsorbent during drought stress reduced the effect of drought stress on color traits, leaf texture quality and aerial part hieght. The application of humic acid and nanocomposite superabsorbent simultaneously improved the density, shoot height, fresh weight, but reduced the ion leakage, proline and dry weight of turfgrass. In general, the results showed that the application of humic and nanocomposite superabsorbent acid could help the stress flow through the passage, and reduced the effect of stress on the plant.


  1. Aalami, M., Tehranifar, A., Davarinejad, Gh. & Selahvarzie, Y. (2011). Effect of hydrogel, paclobutrazol and irrigation intervals on qualitative characteristics of turf grass (Lolium perenne cv. Barbal) in Mashhad climate. Journal of Horticultural Science (Agricultural Sciences and Technology), 25(3), 288-295. (in Farsi)
  2. Ahrar, M., Delshad, M. & Babalar, M. (2009). Improving water/fertilizer use efficiency of hydroponically cultured greenhouse cucumber by grafting and hydrogel amendment. Journal of Horticultural Science (Agricultural Sciences and Technology), 23, 69-77. (in Farsi)
  3. Alikhani, A. (2018). The investigation effects of humic acid on Sport turfgrass under drught and cadmium stresses. MSc Thesis, Faculty of agriculture and Natural Resources, Arak University. (in Farsi) 
  4. Anjum, S. A., Xie, X. Y., Wang, L. C., Saleem, M. F., Man, C. & Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. Africam journal of Agricultural Research, 6(9), 2026-2032.
  5. Arancon, N. Q., Edwards, C. A., Lee, S. & Byrne, R. (2006). Effects of humic acids from vermicomposts on plant growth. European Journal of Soil Biology, 42, 65-69.
  6. Arghavani, M., Kafi, M., Babalar, M. & Nadari, R. (2010). A physiological and morphological study on Lollium perenne L. and Poa pratensis L. as affected by trinexapac-ethyl, mowing management and nitrogen source under salt stress conditions. Ph.D. Thesis, University of Tehran, Karaj, Iran. (in Farsi)
  7. Arnon, A.N. (1967). Metod of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121.
  8. Aronoson, L. J., Gold, A. J. & Hull, R. J. (1987). Cool season turf grass responses to drought stress. Crop Science, 27, 1261-1266.
  9. Bagheri, H. (2016). The evaluation of superabsorbents nanocamposites on growth and resistance to drought and salinity stresses in sport turfgrass. M.Sc. Thesis, Faculty of Agriculture and Natural Resources, Arak University. (in Farsi)
  10. Bates, L. S., Waldren, R. P. & Teare, I. D. (1973). Rapid determination of free prolin for water stress studies. Plant and Soil, 39, 205-207.
  11. Ben Hamed, K., Castagna, A., Salem, E., Ranieri, A. & Abdelly, C. (2007). Sea fennel (Crithmum maritimum L.) under salinity conditions: a comparison of leaf and root antioxidant responses. Plant Growth Regulation, 53, 185-194.
  12. Chamani, E., Esmaeilpour, B., Poorbeiramihir, Y., Malekilajayer, H. & Saadati, A. (2012). Investigation the effects of thidiazouron and humic acid on postharvest life of cut Alstroemeria aurantifolia cv. "Konyambe". Journal of Horticultural Science (Agricultural Sciences and Technology), 26(2), 147-152. (in Farsi)
  13. Chen, Y. & Aviad, T. (1990). Effect of humic substances on plant growth. In: Humic substances in soil and cropsciences. Soil Science Society America, 161-187.
  14. Cordeirom F. C., Santa-Catarina, C., Silveira, V. & Souza, S. R. (2011). Humic acid effect on catalase activity and the generation of reactive oxygen species in corn (Zea Mays L.). Bioscience Biotechnology and Biochemistry, 75, 70-74.
  15. Daneshvar, H. M., Kafi, M., Nikbakht, A. & Rejali, F. (2015). Effect of foliar applications of humic acid on growth, visual quality, nutrients content and root parameters of perennial Ryegrass (Lolium Perenne L.). Journal of Plant Nutrition, 38, 224-236.
  16. Dehgan, B., Yeager, T.H. & Almira, F.C. (1994). Photinin and podocarpus growth response to a hydrophilic polymeramendend medium. Horticultural Science, 29 (6), 641-644.
  17. Esringu, A., Sezen, I., Aytali, B. & Ercisli, S. (2015). Effect of humic and fulvic acid application on growth parameters in Impatiens walleriana L. Akademik Ziraat Dergisi, 4(1), 37-42.
  18. Fan, H. M., Wang, X. W., Sun, X., Li, Y. Y., Sun, W. Z. & Zheng, C. S. (2014). Effects of humic acid derived from sediments on growth, photosynthesis and chloroplast ultrastructure in chrysanthemum. Scientia Horticulturae, 177, 118-123.
  19. Garciaa, A. C., Santos, L. A., Izquierdo, F. G., Sperandio, M. V. L., Castro, R. N. & Berbara, R. L. L. (2012) Vermicompost humic acids as an ecological pathway to protect rice plant against oxidative stress. Ecological Engineering, 47, 203-208.
  20. Ghasemi, M. & Khoshkoi, M. (2007). Effects of superabsorbment polymer on irrigation interval and growth and development of chrysanthemum (Dendranthema×grandiflorum Kitam Syn. Chrysanthemum morifolium Ramat). Iranian Journal of Horticultural Science and Technology, 8(2), 65-65. (in Farsi)
  21. Hosseini, F., Kafi, M. & Arghavani, M. (2016). Effect of Salicylic acid on physiological and morphological characteristics of Lolium perenne cv. “Numan” under drought stress. Iranian Journal of Horticultural Science, 47(2), 167-176. (in Farsi)
  22. Huang, B. & Fu, J. (2001). Growth and physiological responses of tall fescue to surface soil drying.   International Turfgrass Society Research Journal, 9, 291-296.
  23. Hunter, A. & Anders, A. (2004). The influence of humic acid on turfgrass growth and development of creeping bentgrass. Acta Horticulturae, 661, 257-264.
  24. Inze, D. & Van Montagu, M. (1995). Oxidative stress in plants. Current Opinion Biotechnology, 6, 153-158.
  25. Johnson, M.S. & Woodhouse J. 1990. Effect of super absorbent polymers on efficiency of water use by crop seeding. Journal of Science of Food and Agriculture, 52, 431-434.
  26. Kabiri, K. (2002). Acrlic superabsorbent hydrogels. Iranian Polymer and Petrochemical Insitute. (in Farsi)
  27. Kafi, M., Babalar, M., Nikbakht, A., Ebrahimzade, H., Etemadi, N. & Samavat, S. (2009). Effect of humic acid spray on nutrients uptake, protein content and postharvest life of Gerbera jamesonii cv. Malibu. Iranian Journal of Horticultural Science, 40(1), 69-75. (in Farsi)
  28. Kafi, M. & Kavyani, Sh. (2002). Management of establishment and maintenance of turfgrass. Iran, Shaghayeg-e-Roosta Press, 232. (in Farsi)
  29. Karimi, A. (2001). The evaluation of wter superabsorbent improving material on water consumption and plant growth of sunflower. Desert Journal, 6(1), 19-34. (in Farsi)
  30. Kasgo, H., Durmus¸ A. & Kasgoz, A. (2008) Enhanced swelling and adsorption properties of AAm-AMPSNa/clay hydrogel nanocomposites for heavy metal ion removal. Polymers for Advanced Technology, 19, 213-220.
  31. Kenvin, N. & Morris, K. N. (2008). A Guide to NTEP Turfgrass Rating. Retrieved February 25, 2019. From
  32. Kheirabi, J., Tavakoli, A.R., Entesari, M. R. & Salamat, A. R. (1996). Low irrigation manual. Iranian National Committee on Irrigation and Drainage Press, 288. (in Farsi)
  33. Khoshbin, Sh, Solgi, M., Taghizadeh, M. & Barati, A. (2017). The effect of nanocomposite containing zeolite Nanoparticles and some nutrients on the growth characteristics of African violets (Saintpaulia ionantha ‘Rhapsody Clementine’). Iranian Journal of Horticultural Science, 47(4), 835-843. (in Farsi)
  34. Lotfi, R., Gharavi Kouchebagh, P. & Khoshvaghti, H. (2015). Biochemical and physiological responses of brassica napus plants to humic acid under water stress. Russian Journal of Plant Physiology, 62(4), 480-486.
  35. Memon, S. A., Bangulzai, F. M., Keerio, M. İ., Baloch, M. A. & Buriri, M. (2014). Effect of humic acid and iron sulphate on growth and yield of zinnia (Zinnia elegans). Journal of Agricultural Technology, 10(6), 1517-152.
  36. Mohammadi Alborzi, M., Safikhani, F., Masoud Sinaki, J. & Abbaszadeh, B. (2012). The effect of drought on morphological characteristics of anisum (Pimpinella anisum L.). Journal of Plant Ecophysiology, 4(10), 14-25. (in Farsi)
  37. Morris, K. N. (2002). A guide to NTEP turfgrass rating. A publication of the National Turfgrass Evaluation Program. NETP, 11, 30-39.
  38. Panayiotis, A., Nektarios, K., Nikolopoulou, A.E. & Chronopulos, I. (2004). Sod establishment and turf grass growthas affected by urea-formaldehyde resin foam soil amendment. Scientia Horticulture, 100, 203-213.
  39. Sadeghi, A., Etemadi, N., Nikbakht, A. & Sabzalian, M. R. (2015). Effect of drought stress and different levels of shade on the growth and performance of wheatgrass and tall Fescue. Iranian Journal of Horticultural Science, 46(4), 523-534. (in Farsi)
  40. Sanchez-Sánchez, A., Sánchez-Andreu, J., Juárez, M., Jordá, J. & Bermúdez, D. (2002). Humic substances and aminoacids improve effectiveness of chelate FeEDDHA in lemon trees. Plant Nutrition, 25, 2433-2442.
  41. Shafiei, Sh. (2002). The effect of superabsorbent polymer on increasing soil water, fertilizer efficiency, growth and establishment of Panicum. Iranian Polymer and Petrochemical Insitute. (in Farsi)
  42. Shahid, S. A., Qidwai, A. A., Anwar, F., Ullah, I. & Rashid, U. (2012). Improvement in the water retention characteristics of sandy loam soil using a newly synthesized poly (acrylamide-co-acrylic acid)/AlZnFe2O4 superabsorbent hydrogel nanocomposite material. Molecules, 17, 9397-9412.
  43. Sheikhmoradi, F., Arji, I., Emaeili, A. & Abdosi, V. (2011). Evaluation the effects of cycle irrigation and super absorbent on qualitative characteristics of lawn. Journal of Horticultural Science (Agricultural Sciences and Technology), 25(2), 170-177. (in Farsi)
  44. Tango, A., Mahdavi, A. & Sayad, A. (2013). Effects of super absorbent polymers Akoasorb on growth, deployment and some physiological characteristics acacia tree (Acacia victoriae) under drought stress. Journal of Soil and Water (Agricultural Science and Technology), 28(5), 963-951.
  45. Tatari, M., Mousavi, A. & Etemadi, N. (2013). Study of some morphological characteristics in three turf grass species under drought stress conditions. Arid Biome Scientific and Research Journal, 5(2), 12-26. (in Farsi)
  46. Vannozi, G. & Larner, F. (2007). Proline accumulation during drought rhizogene in maise. Journal Plant Physiology, 85, 441-467.
  47. Verlinden, G., Coussens, T., De Vliegher, A. & Baert, G. (2010). Effect of humic substances on nutrient uptake by herbage and on production and nutritive value of herbage from sown grass pastures. Grass and Forage Science, 65, 133.
  48. Wu, F., Zhang, Y., Liu, L. & Yao, J. (2012). Synthesis and characterization of a novel cellulose-g-poly (acrylic acidco-acrylamide) superabsorbent composite based on flaxyarnwaste. Carbohydrate Polymers, 87, 2519-2525.
  49. Xu, R., Yamada, M. & Fujiyama, H. (2013).  Lipid peroxidation and antioxidative enzymes of two turfgrass species under salinity stress. Pedosphere, 23(2), 213-222.
  50. Yadav, M. & Rhee, K. Y. (2012). Superabsorbent nanocomposite (alginate-g PAMPS/MMT): synthesis, characterization and swelling behavior. Carbohydrate Polymers, 90, 165-173.
  51. Yazdani, F., Allah Dadi, A., Akbari, Gh. & Behbahani, M. (2006). Effect amounts of superabsorbent (Tarawat 200) and levels of water stress on yield and yield components of soybean. Pajouhesh-va-Sazandegi, 75(1), 167-174. (in Farsi)
  52. Zandonadi, D. B., Canellas, L. P. & Facmana, A. R. (2007). Indolacetic acid and humic acids induce lateral root development through a concerted plasmalemma and tonoplast H+ pumps activation. Planta, 22, 1583-1595.
  53. Zhang, L., Sun, X. Y., Tian, Y. & Gong, X. G. (2014). Biochar and humic acid amendments improve the quality of composted green waste as a growth medium for the ornamental plant Calathea insignis. Scientia Horticulturae, 176, 70-78.