Effect of arbuscular mycorrhizal fungus treatments on growth and some nutrient ‎elements uptake of grapevine cv ‘Rasha’ (Vitis vinifera L.) under deficit irrigation ‎stress condition

Document Type : Full Paper


1 Associate Professor, Horticulture Crops Research Department, West Azarbaijan Agricultural and Natural Resources Research and ‎Education Center, AREEO, Urmia, Iran ‎

2 Instrcutor, Soil and Water Research Department, West Azarbaijan Agricultural and Natural Resources Research and Education ‎Center, AREEO, Urmia, Iran

3 Assistant Professor, Agricultural Engineering Research Department, West Azarbaijan Agricultural and Natural Resources Research ‎and Education Center, AREEO, Urmia, Iran


To determaine th effect of root inoculation of Rasha grape cultivar with three species of arbuscular mycorrhizal fungus(G.versiforme, G. etunicatum, G. intraradices) on shoot growth, macro nutrient elements, chlorophyll content, leaf temperature, RWC, soluble sugars and drought symptoms rate under deficiet irrigation condition (75%, 50% and 35% water requirment) a factorial experiment based on RBCD was conducted in Kahriz Horticultural Research Station, Urmia, Iran. Results showed that increasing deficiet irrigation level, reduced shoot growthand fungus inoculation did not have a posetive effect on this trait in comparision with control. Leaf temperature and dry symptoms on leaves was lowest in vines that inoculated with G. versiforme under 35% deficiet irrigation condition. Leaves of vines with this fungus showed the high RWCin all stress tretments, too. By increasing deficiet irrigation level, soluble sugars were decreased in 55% water requirement and then increased in 35% water requirement. Among macro nutrient elements, the highest amount of P (0.14%) was measured in leaves of vines inoculated by G. versiforme


  1. Ahmadi, A. & Biker, D.A. (2000). Stomatal and nonstomatal limitations of photosynthesis under water stress conditions in wheat plant. Iranian Journal of Agricultural Science, 31(4), 813-825. (in Farsi)
  2. Ahmadi, A. & Sio-semarde, A. (2004). The effects of water stress on soluble carbohydrates, chlorophyll and proline contents of four Iranian wheat cultivars under different moisture regimes. Iranian Journal of Agricultural Science, 35(3), 753-763. (in Farsi)
  3. Anonymous. (2015). West Azerbijan horticultural vision. Jahade-Agricultur organization of West Azerbijan. 34 p. 
  4. Aslanpour, M., Dolati Baneh, H., Tehranifar, A. & Shoor, M. (2016). The effect of mycorrhizal fungi on the amount of glycine betaine, soluble sugar, proline, leaf water content and leaf chlorophyll of the white seedless grape under drought stress conditions. International Journal of Advanced Biotechnology and Research (IJBR), 7(3), 1119-1133.
  5. Babu, H. K. & Prakash, G. S. (2003). Effect of water stress and rootstocks on leaf mineral composition of grape. Indian Journal of Horticulture, 60, 147-150.
  6. Barkoki, M., Hifnny, H.A. & Baghdad, G.A. (1997). Some effects of water stress on growth of grape vine Vitis vinifera. Acta Horticulture, 84, 199-214.
  7. Baumgartner, K. (2006). The role of beneficial mycorrhizal fungi in grapevine nutrition. ASEV Technical update, 1(1), 3.
  8. Bavaresco, L. & Fogher, C. (1996). Lime-induced chlorosis of grapevine as affected by rootstock and root infection with arbuscular mycorrhiza and Pseudomonas fluorescens. Vitis, 35 (3), 119-123.
  9. Ben- Asher, J., Tsuyuki, I., Bravdo, B. A. & Sagih, M. (2006). Irrigation of grapevines with saline water, I. leaf area index, stomatal conductance, transpiration and photosynthesis. Agricultural Water Management, 83, 13-21.
  10. Emami, A. (1997). Plant Analysis Methods. Agricultural Research, Education and Extension Organization Publication, Iran. 128 pp. (in Farsi).
  11. Fisarakis, I., Chartzoulakis, K. & Stavrakas, D. (2001). Response of Sultana vines (V. vinifera L.) on six rootstocks to NaCl salinity exposure and recovery. Agricultural Water Management, 51, 13-27.
  12. Ghaderi, N., Sio-semarde, A. & Shahoee, S.S. (2006). Effects of drought stress on some physiological traits of two grapevine cultivars. Iranian Journal of Agricultural Science, 29(1), 45-55. (in Farsi)
  13. Hedari Sharifabad, H. (2000). Plants, dryness, drought. Research Institute of Forest and Rengelands Publication. P.200.
  14. Hokekstra, F.A. & Buiting, J. (2001). Mechanisms of plant desiccation tolerance. Trends plant Science, 8, 431-438.
  15. Hsiao, T. C. (1973). Plant responses to water stress. Annual Reviwe of Plant Physiology, 24, 519-570.
  16. Irigoyen, J. J., Emerich, D. W. & Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84, 55-60.
  17. Keller, M. (2010). The Science of Grapevines. Academic Press: Elsevier. London, UK. 377 p.
  18. Kerepesi, I., Galiba, G. & Banyal, E. (1998). Osmotic and salt stresses induced differential alteration in water-soluble carbohydrate content in wheat seedlings. Journal of Agricultural Food Chemistry, 46, 5347-5354.
  19. Linderman, R.G. & Davis, E.A. (2001). Comparision response of selected grapevine rootstock and cultivars to inoculation with different mycorrhizal fungi. American Journal of Enology and Viticulture, 52, 8-11.
  20. Mannini, P. & Anconeli, S. (2002). Leaf temperature and water stress in strawberry. American Journal of Enology and Viticulture, 53(2), 138-143.
  21. Martinez-Barroso, M.C & Alvarez, C.E. 1997. Toxicity symptoms and tolerance of strawberry to salinity in the irrigation water. Scientia Horticulture, 71, 177-188.
  22. Marulanda, A., Azcón, R. & Ruiz-Lozano, J.M. (2003). Contribution of six arbuscular mycorrhizal fungal isolates to water uptake by Lactuca sativa plants under drought stress. Physiologia Plantarum, 119(4), 526-533.
  23. Menga, J, A., Raski, D. J., Lider, L. A., Johnson, E. L., Jones, N. O., Kissler, J. J. & Hemstreet, C. (1983). Interaction between mycorrhizal fungi, soil fumigation and growth of grapes in California. American Journal of Enology and Viticulture, 34, 117-121.
  24. Motosugi, h., Yamamoto, Y., Naruo, T., Kitabayashi, H & Ishii, T. (2002). Comparision of the growth and leaf mineral concentration between three grapevine rootstocksand their corresponding tetraploids inoculated with an arbuscular mycorrhizal fungus Gigaspora margarita. Vitis, 41, 21-25.
  25. Newton, R.J., Bhaskaran, S., Puryear, J. D. & Smith, R. H. (1986). Physiological changes in cultured sorghum cells in response to induced water stress. II. Soluble carbohydrates and organic acids. Plant Physiology, 81, 626-629.
  26. Nikolaou, N., Angelopoulos, K. & Karagiannidis, N. (2003). Effects of drought stress on mycorrhizal and non- mycorrhizal Cabernet Sauvignon grapevines, grafted on to various rootstocks. Experimental Agriculture, 39, 241-252.
  27. Phillips, J.M. & Hayman, D.S. (1970). Improved Procedures for Clearing Roots and Staining Parasitic Vesicular-Arbuscular Mycorrhizal Fungi for Rapid Assessment of Infection. Transactions of the British Mycological Society, 55, 158-161.
  28. Rezaee, T. (2006). Evaluation of some physiological characteristics of five grape varieties for selection of drought tolerant cultivars. M.Sc. Thesis, University of Hamadan, Iran. 61p. (in Farsi)
  29. Rooyen, M., Valentine, A. & Archer, E. (2004). Arbuscular mycorrhizal colonisation modifies the water relations of young transplanted grapevines (Vitis). South African Enology and Viticulture. 25(2), 37-42.
  30. Schreiner, R.P. (2005). Mycorrhizas and mineral acquisition in grapevines. American Society of Enology and Viticulture. Davis, CA, pp 49-60.
  31. Schreiner, R. P. (2007). Effects of native and nonnative arbuscular mycorrhizal fungi on growth and nutrient uptake of ‘Pinot noir’ (Vitis vinifera L.) in two soils with contrasting levels of phosphorus. Applied Soil Ecology, 36, 205-215.
  32. Sedlaček, M., Pavloušek, P., Lošak, T., Zatloukalova, A., Filipčik, R., Hlušek, J & Vitězova, M. (2013). The effects of arbuscular mycorrhizal fungi on the content of macro and micro elements in grapevine (Vitis vinifera L.) leaves. Acta Universitatis Agriculture et Silviculture Mendeliane Brunensis, 1, 187-191.
  33. Shahsavandi, F. & Eshghi, S. (2016). Effect of mycorrhizal fungus on some growth and physiological indeices in ‘Perllete’ grapevine under high temperature stress. Iranian Journal of Horticultural Science, 47(3), 553-557. (in Farsi)
  34. Thakur, P. S. & Rai, V. K. (1980). Water stress effects on maize: Carbohydrate metabolism of resistant and susceptible cultivars of Zea mays L. Biologia Plantarum, 21(1), 50-56.
  35. Turner, N. C. (1981). Techniques and experimental approaches for the measurement of plant water status. Plant and Soil, 58, 339-366.
  36. Torres, N., Goicoechea, N., Zamarreño, A.M. & Carmen Antolín, M. (2018). Mycorrhizal symbiosis affects ABA metabolism during berry ripening in Vitis vinifera L. cv. Tempranillo grown under climate change scenarios. Plant Science, 274, 383-393.