Effects of drought and salinity stresses on some morphological and physiological ‎characteristics of Narcissus tazetta L. flower

Document Type : Full Paper


1 M. Sc. Student, Faculty of Agriculture, University of Birjand, Birjand, Iran

2 Assistant Professor, Faculty of Agriculture, University of Birjand, Birjand, Iran

3 Associated Professor, Faculty of Agriculture, University of Birjand, Birjand, Iran


Drought and salinity are among the most important factors limiting plant growth in many parts of the world. The purpose of this study was to investigate the interaction effects of drought and salinity stresses on some morphological and physiological factors of Narcissus tazetta L. This pot experiment was conducted as a factorial based on completely randomized design with three replications at the Faculty of Agriculture, University of Birjand in 2017.The treatments included sodium chloride (NaCl) in four levels 0 (control), 20, 40 and 60 mM and drought stress in four levels 30, 50, 70 and 90% of field capacity.The results showed that salinity and drought stresses decreased length and width of leaf, dry weight of shoot and bulb, relative water content and chlorophyll content. However, the effects of salinity and drought stress and their interaction on reducing the number of flowers in flowering stem were not significant. In contrast, electrolyte leakage and proline content were increased under these two stresses. In general, the results showed that in the range of applied treatments, the destructive effects of salinity stress on growth, ornamental and physiological traits of N. tazetta flower were more than drought stress.


  1. Abedi, T. & Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46(1), 27-34.
  2. Agricultural Statistics of the Ministry of Agriculture Jihad, 2016. (in Farsi)
  3. Araus, J. L., Slafer, G. A., Reynolds, M. P. & Royo, C. (2002). Plant breeding and drought in C3 cereals: What should we breed for. Annals of Botany, 89, 925-940.
  4. Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121.
  5. Babaee, K., Amini Dehaghi, M., Modares Sanavi, S. A. M. & Jabbari, R. (2010). Water deficit effect on morphology, prolin content and thymol percentage of thyme (Thymus vulgaris L.). Scientific Journal Management System, 26(2), 239-251.
  6. Bahadoran, M. & Salehi, H. (2015). Growth and flowering of two tuberose (Polianthes tuberosa L.) cultivars under deficit irrigation by saline water. Journal of Agricultural Science and Technology, 17(2), 415-426.
  7. Barranco, D., Ruiz, N. & Gomes, M. (2005). Frost tolerance of eight olive cultivars. HortScience, 40, 558-560.
  8. Barrs, H. D. & Weaterley, P. E. (1962). A re-examination of the relative turgidity techniques for the estimating water deficit in leaves. Australian Journal of Biological Sciences, 15, 413-428.
  9. Bates, L. S., Waldern, R. P. & Teave, I. D. (1973). Rapid determination of free proline for water stress standies. Plant and Soil, 39, 205-107.
  10. Bayat, H., Nemati, H., Tehranifar, A. & Gazanchian, A. (2016). Screening different crested wheatgrass (Agropyron cristatum (L.) Gaertner.) accessions for drought stress tolerance. Archives of Agronomy and Soil Science, 62 (6), 769-780.
  11. Bettaieb, I., Zakhama, N., Wanes, W. & Marzouk, B. (2009). Water deficit effects on (Salvia officinalis) fatty acids and essential oils composition. Scientia Horticulturae, 120(2), 271-275.
  12. Caser, M., D’Angiolillo, F., Chitarra, W., Lovisolo, C., Ruffoni, B., Pistelli, L., Pistelli, L. & Scariot, V. (2018). Ecophysiological and phytochemical responses of Salvia sinaloensis Fern. to drought stress. Journal of Plant Growth Regulation, 84, 383-394.
  13. Dole, J.M. & Wilkins, H.F. (2005). Floriculture, principles and species. Prentice-Hall, Inc. U.S.A.
  14. Egert, M. & Tevini, M. (2002). Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environmental and Experimental Botany, 48, 43-49.
  15. Gerailoo, S., Ghasemnezhad, M. & Shiri, M. (2014). Effect of short time treatment of salicylic acid in delaying flowers senescence in cut rose (Rosa hybrida) cv. Yellow Island. Journal of Plant Researches, 27(2), 299-309.
  16. Ghasemi Phirouzabadi, A. A., Jafari, M., Heidari sharifabad, H., Azarnivand, H. & Abbasi H. R. (2001). Investigation of the morphologic- physiologic changes of Puccinelia distance and Aeluropus littoralis to salinity and drought resistance. Iranian Journal of Range and Desert Reseach, 16, 1-10. (in Farsi)
  17. Guerfel, M., Baccouri, O., Boujnah, D., Chaibi, W. & Zarrouk, M. (2009). Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Scientia Horticulturae, 119, 257-263.
  18. Hajmohammadnia Ghalibaf, K. & Selahvarzi, Y. (2012). Physiological responses of kallar grass (Leptochloa fesca L.) to combined salinity and drought stresses under controlled conditions. Environmental Stresses in Crop Sciences, 4(2), 105-115.
  19. Huang, B. & Fu, J. (2001). Growth and physiological responses of tall fescue to surface soil drying. International Turfgrass Society Research Journal, 9, 291-296.
  20. Kinghorn, A. D. (1987). Biologically active compounds from plants with reputed medicinal and sweetening properties. Journal of Natural Products, 50(6), 1009-1024.
  21. Li, X.-F., Shao, X.-H., Deng, X.-J., Wang, Y., Zhang, X.-P., Jia, L.-Y. & Xu, L. (2012). Necessity of high temperature for the dormancy release of Narcissus tazetta var. chinensis. Journal of Plant Physiology, 169(14), 1340-1347.
  22. Mahajan, S. & Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics, 444(2), 139-158.
  23. Maggio, A., Miyazaki, S., Veronese, P., Fujita, T., Ibeas, J. I., Damsz, B., Narasimhan, M. L., Hasegawa, P. M., Joly, R. J. & Bressan, R. A. (2002). Does proline accumulation play an active role in stress-induced growth reduction?. Plant Journal, 31, 699-712.
  24. Nakhaei, F., Khalighi, A., Naseri, M. & Abroumand, P. (2008). The investigation of chemical components in essential oil of Narcissus tazetta L. flowers under farm and natural conditions in south khorasan. Journal of Horticulture Science, 22, 123-131. (in Farsi)
  25. Pessarakli, M. & Szabolcs, I. (1999). Soil salinity and sodicity as particular plant/crop stress factors. In: M. Pessarakli (Ed) Handbook of plant and crop stress. (pp. 1–16). Dekker, New York.
  26. Pessarakli, M. & Kopec, D. M. (2008). Comparing growth responses of selected cool-season turfgrasses under salinity and drought stresses. Turfgrass, landscape and urban ipm research summary. P-155.
  27. Richards, R. A. (1996). Defining selection criteria to improve yield under drought. Plant growth regulation, 20, 157-166.
  28. Sabet Teimouri, M ., Kafi, M., Avarseji, Z . & Orooji, K. (2011). Effect of drought stress, corm size and corm tunic on morphoecophysiological characteristics of saffron (Crocus sativus L.) in greenhouse conditions. Agroecology, 2(2), 323-334. (in Farsi)
  29. Sadeghi, H. & Khani, K. (2012). Effects of different drought and salinity stress levels on some morphological characteristics and proline content of annual burr medics (Medicago polymorpha L.). Iranian Dryland Agronomy Journal, 1(2), 1-13. (in Farsi)
  30. Sanchez-Blanco, M. J., Rodriguez, P., Olmos, E., Morales, M. A. & Torrecillas, A. (2004). Differences in the effects of simulated sea aerosol on water relations, mineral content and ultrastructural in Cistus albidus and Cistus monspeliensis plants. Journal Environment Quality, 33, 1369-1375.
  31. Sepaskhah, A. R. & Yarami, N. (2009). Interaction effects of irrigation regime and salinity on flower yield and growth of saffron. Journal of Horticultural Science and Biotechnology, 84, 216-222.
  32. Shahbazi, H., Arzani, A. & Esmaelzadeh Moghadm, M. (2016). Effects of drought stress on physiological characteristics in wheat recombinant inbred lines. Journal of Plant Process and Function, 5(15), 123-132. (in Farsi)
  33. Shao, H. B., Chu, L. Y., Jaleel, C. A. & Zhao, C. X. (2008). Water-deficit stress-induced anatomical changes in higher plants, Comptes Rendus Biologies, 331, 215-225.
  34. Shillo, R., Ding, M., Pasternak, D. & Zaccai, M. (2002). Cultivation of cut flower and bulb species with saline water. Scientia Horticulturae, 92, 41-54.
  35. Soleimani, S., Bernard, F., Amini, M. & Khavari-nezhad, R. (2007). Alkaloids from Narcissus tazetta L. Journal of Medicinal Plants, 4 (24), 58-63. (in Farsi)
  36. Sreenivasulu, N., Grimm, B., Wobus, U. & Weshke, W. (2000). Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica). Physiologia Plantarum, 109(4), 435-442.
  37. Tabatabae, S.A., Ghasemi, A. & Shakeri, E. (2012). Effect of water stress on yield, yield components and oil quantity of canola cultivars. Crop Physiology Journal, 3(12), 41-53. (in Farsi)
  38. Ueda, A., Kanechi, M. & Uno, Y. (2003). Photosynthetic limitations of a halophyte sea aster (Aster tripolium L.) under water stress and NaCl stress. Journal of Plant Research, 116(1), 63-68.
  39. van Dort, H. M., Jagers, P. P., ter Heide, R., & van der Weerdt, A. J. (1993). Narcissus trevithian and Narcissus geranium: analysis and synthesis of compounds. Journal of Agricultural and Food Chemistry, 41(11), 2063-2075.
  40. Veatch-Blohm, M. E., Chen, D. & Hassett, M. (2013). Narcissus cultivar differences in response to saline irrigation when application began either pre- or postemergence. Hortscience, 48(3), 322-329.
  41. Yousef, A. M. (2009). Salt tolerance mechanisms in some halophytes from saudi arabia and egypt. Research Journal of Agriculture and Biological Sciences, 5, 191-206.
  42. Zhang, X., Zhang, L., Dong, F., Gao, J., Galbraith, D. W. & Song, C. P. (2001). Hydrogen peroxide is involved in abscisic acid- induced stomatal closure in Vicia faba. Plant Physiology, 126, 1438-1448.