Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effects of different concentrations of CO2 in the atmosphere of in vitro culture ‎vessels on growth characteristics and photosynthetic capacity of Epipactis ‎veratrifolia

Document Type : Full Paper

Authors
Zohre Soltani 1
Shirin Dianati Daylami 2
Sasan Aliniaeifard 2
Saeideh Rostami 3
1 M. Sc. Student, Department of Horticulture, College of Aburaihan, University of Tehran, Pakdasht, ‎Tehran, Iran
2 Assistant Professor, Department of Horticulture, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran
3 M. Sc. Student , Department of Horticulture, College of Aburaihan, University of Tehran, Pakdasht, Tehran, ‎Iran
10.22059/ijhs.2019.250705.1386
Abstract
Epipactis veratrifolia is a terrestrialorchid that belongs to thetemperate zones of Iran with great breeding potentials for ornamental usages. Although in vitro propagation of this orchid has been previously performedbut investigation on factors influencing its in-vitro growth and photosynthesis is still needed. In the current study, three-leaflet seedlings were cultured in closed vessels containing modified Fast liquid medium together with sterilized perlite (for sample establishment). To remove CO2, vials containing saturated KOH solution as CO2 absorbent and to increase CO2 concentrations, vials containing 3 mL CO2-releasing solutions (3 M sodium bicarbonate and sodium carbonate) were placed into the culture vessels. The experiment was carried out based on a completely randomized design with three replications and three samples per each replicate. Growth parameters including fresh and dry weights of shoot, stem height and root length together with transpiration rate and maximum quantum yield of photosystem II efficiency (QYmax) of the samples were measured. The highest values for shoot height, shoot dry and fresh weights and root length were observed in CO2-increased treatments. Removing CO2 from the atmosphere of culture vessels caused a dramatic decrease in QYmax of plantlets in vitro. Meanwhile, following desiccation, higher transpiration rate was detected in control and CO2-increased treatments, while removing CO2 decelerate transpiration rate of the plantlets. In conclusion, with increasing CO2 in vitro we can promote photosynthetic efficiency of Epipactis veratrifolia and as a result enhance growth of plantlets in vitro.
Keywords
Carbon dioxide absorbent
carbon dioxide releasing
in vitro culture
maximum quantum yield of photosystem II ‎efficiency (QYmax)‎
  1. Aliniaeifard, S. & van Meeteren, U. (2014). Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning. Journal of Experimental Botany, 65(22), 6529-6542.
  2. Arigita, L., González, A. & Tamés, R. S. (2002). Influence of CO2 and sucrose on photosynthesis and transpiration of Actinidia deliciosa explants cultured in vitro. Physiologia Plantarum, 115(1), 166-173.
  3. Askari, N. (2016). Aspects of bulblet growth of lily in vitro. wageningen university. PhD. thesis. ISBN:978-94-6257-760-2
  4. Brainerd, K. & Fuchigami, L. (1982). Stomatal functioning of in vitro and greenhouse apple leaves in darkness, mannitol, ABA, and CO­2. Journal of Experimental Botany, 33(3), 388-392.
  5. Dangwal, L., Sharma, A., Kumar, N., Rana, C. & Sharma, U. (2010). Ethno-medico botany of some aquatic Angiospermae from North-West Himalaya. Researcher, 2(4), 49-54.
  6. Deng, R. & Donnelly, D. J. (1993). In vitro hardening of red raspberry by CO2 enrichment and reduced medium sucrose concentration. HortScience, 28(10), 1048-1051.
  7. Desjardins, Y., Hdider, C. & de Riek, J. (1995). Carbon nutrition in vitro—regulation and manipulation of carbon assimilation in micropropagated systems Automation and environmental control in plant tissue culture (pp. 441-471): Springer.
  8. Dogan, Y., Baslar, S., Ay, G. & Mert, H. H. (2004). The use of wild edible plants in western and central Anatolia (Turkey). Economic Botany, 58(4), 684-690.
  9. Durchan, M., Vácha, F. & Krieger-Liszkay, A. (2001). Effects of severe CO2 starvation on the photosynthetic electron transport chain in tobacco plants. Photosynthesis research, 68(3), 203.
  10. Fast, G. (1976). Moglichkeiten zur Massenvermehrung von Cypripedium calceolus und anderen europaischen Wildorchideen. Paper presented at the Proceedings of the Eighth World Orchid Conference. Frankfurt, Germany: German Orchid Society (in German).
  11. Galzy, R. & Compan, D. (1992). Remarks on mixotrophic and autotrophic carbon nutrition of Vitis plantlets cultured in vitro. Plant cell, tissue and organ culture, 31(3), 239-244.
  12. Genty, B., Briantais, J.-M. & Baker, N. R. (1989). The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects, 990(1), 87-92.
  13. Haisel, D., Pospíšilová, J., Synková, H., Čatský, J., Wilhelmová, N. & Plzáková, Š. (1999). Photosynthetic pigments and gas exchange of in vitro grown tobacco plants as affected by CO2 supply. Biologia Plantarum, 42(3), 463-468.
  14. Hazarika, B. (2006). Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae, 108(2), 105-120.
  15. Hazarika, B., Parthasarathy, V. & Nagaraju, V. (2004). Influence of in vitro preconditioning of Citrus sp. microshoots with sucrose on their ex vitro establishment. Indian Journal of Horticulture, 61(1), 29-31.
  16. Kozai, T. (1991). Photoautotrophic micropropagation. In Vitro Cellular & Developmental Biology-Plant, 27(2), 47-51.
  17. Kozai, T. (2010). Photoautotrophic micropropagation—environmental control for promoting photosynthesis. Prop Ornam Plants, 10, 188-204.
  18. Kozai, T., Iwabuchi, K., Watanabe, K. & Watanabe, I. (1991). Photoautotrophic and photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium. Plant cell, tissue and organ culture, 25(2), 107-115.
  19. Kozai, T. & Iwanami, Y. (1988). Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophyllus L.) in tissue culture during the preparation stage. Journal of the Japanese Society for Horticultural Science, 57(2), 279-288.
  20. Kozai, T., Koyama, Y. & Watanabe, I. (1988). Multiplication of potato plantlets in vitro with sugar free medium under high photosynthetic photon flux. Paper presented at the Symposium on High Technology in Protected Cultivation 230.
  21. Kozai, T., Oki, H. & Fujiwara, K. (1987). Effects of CO2 enrichment and sucrose concentration under high photosynthetic photon fluxes on growth of tissue-cultured Cymbidium plantlets during the preparation stage. Paper presented at the Symposium: Plant micropropagation in horticultural industries. Preparation, hardening and acclimatization processes, Arlon (Belgium), 10-14 Aug 1987.
  22. Kozai, T., Takazawa, A., Watanabe, I. & Sugi, J. (1990). Growth of tobacco seedlings and plantlets in vitro as affected by in vitro environment. Environment Control in Biology, 28(2), 31-39.
  23. Lake, J. & Woodward, F. (2008). Response of stomatal numbers to CO2 and humidity: control by transpiration rate and abscisic acid. New Phytologist, 179(2), 397-404.
  24. Mehler, A. H. (1951). Studies on reactions of illuminated chloroplasts: I. Mechanism of the reduction of oxygen and other hill reagents. Archives of Biochemistry and Biophysics, 33(1), 65-77.
  25. Moradi, S., Dianati daylami, S., Vahdati, K. & Arab, M. (2016). Effect of medium, explants and BA on somatic embryogenesis induction in tow Iranian native orchids. Journal of Plant Production Research, 22(4), 119-132.  (in Farsi)
  26. Nguyen, Q. T. & Kozai, T. (2005). Photoautotrophic Micro-Propagation of Woody Species. In T. Kozai, F. Afreen & S. M. A. Zobayed (Eds.), Photoautotrophic (sugar-free medium) Micropropagation as a New Micropropagation and Transplant Production System pp. Dordrecht: Springer Netherlands.
  27. Nobel, P. (1991). Physiological and environmental plant physiology. Academic, New York.
  28. Norikane, A., Teixeira da Silva, J. A. & Tanaka, M. (2013). Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps with super-elevated CO2 enrichment. AoB Plants, 5, plt044.
  29. Pospisilova, J., Synková, H., Haisel, D. & Semorádová, S. (2007). Acclimation of Plantlets to Ex Vitro Conditions: Effects of Air Humidity, Irradiance, CO2 Concentration and Abscisic Acid (a Review). Acta horticulturae, 748, 29.
  30. Reddy, A. R., Rasineni, G. K. & Raghavendra, A. S. (2010). The impact of global elevated CO₂ concentration on photosynthesis and plant productivity. Current Science, 46-57.
  31. Rkhis, A. C., Maalej, M., Drira, N. & Standardi, A. (2011). Micropropagation of olive tree Olea europaea L.'Oueslati'. Turkish Journal of Agriculture and Forestry, 35(4), 403-412.
  32. Rybczyński, J. J., Borkowska, B., Fiuk, A., Gawrońska, H., Śliwińska, E. & Mikuła, A. (2007). Effect of sucrose concentration on photosynthetic activity of in vitro cultures Gentiana kurroo (Royle) germlings. Acta Physiologiae Plantarum, 29(5), 445-453.
  33. Saldanha, C. W., Otoni, C. G., Notini, M. M., Kuki, K. N., da Cruz, A. C. F., Neto, A. R., Otoni, W. C. (2013). A CO2-enriched atmosphere improves in vitro growth of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. In Vitro Cellular & Developmental Biology-Plant, 49(4), 433-444.
  34. Schreiber, U. & Neubauer, C. (1990). O2 dependent electron flow, membrane energization and the mechanism of non-photochemical quenching of chlorophyll fluorescence. Photosynthesis research, 25(3), 279-293.
  35. Solárová, J. & Pospíšilová, J. (1997). Effect of carbon dioxide enrichment during in vitro cultivation and acclimation to ex vitro conditions. Biologia Plantarum, 39(1), 23-30.
  36. Vahdati, K., Asayesh, Z. M., Aliniaeifard, S. & Leslie, C. (2017). Improvement of Ex Vitro Desiccation through Elevation of CO2 Concentration in the Atmosphere of Culture Vessels during In Vitro Growth. HortScience, 52(7), 1006-1012.
  37. Xiao, Y., Niu, G. & Kozai, T. (2011). Development and application of photoautotrophic micropropagation plant system. Plant Cell, Tissue and Organ Culture (PCTOC), 105(2), 149-158.
Iranian Journal of Horticultural Science
Volume 51, Issue 1
Spring 2020
Pages 45-54

  • Receive Date 24 January 2018
  • Revise Date 11 February 2019
  • Accept Date 02 March 2019