Document Type : Full Paper
Ph.D. Candidate, Faculty of Agriculture, Tabriz University, Tabriz, Iran
Associate Professor, Agricultural and Natural Resources University, Sari, Iran
Assistant Professor, Genetics Research Center, Agricultural and Natural Resources University, Sari, Iran
Associate Professor, Faculty of Agriculture, Tabriz University, Tabriz, Iran
In the present study, hairy root induction from leaf and stem explants in two Hyssopus species (H. officinalis and H. angustifilius) was assessed using four Agrobacterium rhizogenes strains including A4, ATCC15834, LB9404 and 2656 on three culture media (MS, ½ MS, B5) as a factorial experimental based on a completely randomized design with three replicates. Almost all leaf and stem explants produced hairy roots. A fragment of transferred rol B was amplified through PCR from the genomic DNA extracted from transformed roots. Different A. rhizogenes strains and culture media and Hyssopus species had significant effects on hairy root percentage and root length. The highest rooting percentage (%71) and root length (4.7 cm) were resulted from stem explants of H. angustifolius co-cultivated with the strain ATCC15834 on MS culture medium. While, the leaf explantsof H. officinalis co-cultivated with the strain A4 on MS culture medium showed the rooting percentage of 55% and the root length of 1.6 cm at most. The MS medium was the best culture medium for both species. These results can be useful in genetic manipulation of hyssop and its hairy root culture to produce high-value secondary metabolites.