Effect of culture media and plant growth regulators on micropropagation of two ‎Prunus rootstocks (CAB 6p and JASPI) ‎

Document Type : Full Paper


1 Associate Professor, Temperate and Cold Fruits Research Institute (TCFRI), Horticulture Science Research Institute, Karaj, Iran

2 Former M.Sc. Student, Islamic Azad University, Karaj Branch, Karaj, Iran‎

3 Associate Professor, Institute of Agriculture, Department of Plant Production, Iranian Research Organization for Science and ‎Technology (IROST), Tehran, Iran‎

4 M.Sc., Temperate and Cold Fruits Research Institute (TCFRI), Horticulture Science Research Institute, Karaj, Iran‎


The CAB 6P and JASPI rootstocks belongs to Prunus genus, are very noticeable in terms of dwarfing. Due to the importance of dwarf rootstocks in fruit trees commercial production and also their mass propagation by tissue culture, this research was conducted to study the proliferation, rooting and acclimatization of these rootstocks. A factorial experiment was carried out based on a completely randomized design, including the effect of MS and ½ MS media in combination with different concentrations of BA (0, 0.5, 1 and 1.5 mg/l) and IBA (0, 0.1 and 0.2 mg/l) on proliferation and also the effect of WPM and MS media in combination with different concentrations of IBA and NAA (0, 0.5, 1 and 1.5 mg/l) on rooting of two rootstock (JASPI and CAB 6P). Based on the results, MS medium with 1 mg/l BA + 0.2 mg/l IBA for proliferation of both rootstocks and WPM medium containing 1.5 mg/l IBA + 0.5 mg/l NAA and WPM medium containing 0.5 mg/l IBA+ 0.5 mg/l NAA are recommended for rooting stage of CAB 6P and JASPI rootstocks, respectively.


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