Effect of different levels of salinity on growth indices, mineral absorption, antioxidant enzymes activity and some physiological traits of roots and leaf in GN15 hybrid rootstocks

Document Type : Full Paper


1 Former M. Sc. Student, Faculty of Agriculture, University of Tabriz, East Azarbaijan, Iran

2 Professor, Faculty of Agriculture, University of Tabriz, East Azarbaijan, Iran

3 Research, East Azarbaijan Agricultural Research Center, Iran


In order to investigate the effects of different levels of salinity on growth indices, proline and chlorophyll content, nitrogen concentration, potassium, phosphorus and sodium contents of leaf and sodium concentration of root of GN15 hybrid rootstocks, as well as the activity of three antioxidant enzymes, an experiment was conducted based on a completely randomized design with 4 salinity levels of 0, 35, 70 and 105 mg / L sodium chloride and 5 replicates in 1394. Different concentrations of sodium chloride were added to the pots containing these plants and after 100 days of salinity stress, these traits were measured. Results showed as increasing salinity levels, growth indices including plant length and diameter, leaf number and area, relative humidity content and leaf chlorophyll content decreased. This reduction was lower at concentration of 35 mm but was very severe at 105 mM. Concentration and efficiency uptake of nitrogen, potassium and phosphorus decreased with increasing sodium chloride concentrations, and increased the concentration of sodium in leaves and roots and its uptake efficiency in leaves. The amount of proline in the root and leaf increased simultaneously with increasing salt concentrations, so that its amount reached a maximum at concentration of 70 mM (39 and 30 mg / g fresh weight). The activity of antioxidant enzymes was also increased by increasing the salt concentration up to 70 mM, so that SOD, POD and CAT of roots in this treatment were 150.2, 22.6,13 and for leavs were 183.8, 16.8 and 24 U/mg protein, respectively.


Main Subjects

  1. Abdul Jaleel, C., Riadh, K., Gopi, R., Manivannan, P., Ines, J., Al-Juburi, H. J., Chang-Xing, Z., Hong-Bo, S. & Panneerselvam, R. (2009). Antioxidant defense responses: physiological plasticity in higher plants under abiotic constrains. Acta Physiologia Plantarum, 31, 427-436.
  2. Abili, J. & Zare, S. (2014). Evaluation of antioxidant enzymes activity in canola under salt stress. International Journal of Farming and Allied Sciences, 7, 767-771.
  3. Alizadeh, M., Singh, S. K., Patel, V. B., Bhattacharya, R. C. & Yadav, B. P. (2010). In vitro responses of grape rootstocks to NaCl. Biologia Plantarum, 54, 381-385.
  4. Amini, Z. & Saadati, S. (2014). Effects of water deficit on proline content and activity of antioxidant enzymes among three olive (Olea europaea L.) cultivars. Journal of Plant Research, 27(2), 156-167. (in Farsi)
  5. Arnon, D. I. (1949). Copper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1-15.
  6. Ashraf, M. & Ali, Q. (2008). Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environmental and Experimental Botany, 63, 266-273.
  7. Ashraf, M. & Harris, P. J. C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3-16.
  8. Bagherzadeh, A., Kavousi, H., Khezri, M. & Mirzaei, S. (2016). Study of protein expression pattern and some morphological and biochemical characteristics of Badami-Sefid and Badami-Zarand pistachio rootstocks under salt stress. Agricultural Biotechnology Journal, 8(3), 15-32. (in Farsi)
  9. Balal, R. & Khan, M. (2012). Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl stress. Journal of the American Society of Horticultural Science, 137(2), 86-95.
  10. Bates, L. S., Waldren, R. P. & Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-207.
  11. Bolat I., Kaya C., Almaca A. & Timucin, S. (2006). Calcium sulfate improve salinity tolerance in rootstock of plum. Journal of Plant Nutrition, 29, 553-564.
  12. Dejampoor, G., Aliasgarzadeh, N., Grigorian, V. & Majidi-Hervan, A. (2012). Evaluation of salinity tolerance in some interspecific hybrids of prunus. Seedling and Seed Breeding, 28(3), 339-351. (in Farsi)
  13. Duran-Zuazo, V. H., Martinez-Raya, H. & Aguilar-Ruiz, J. (2003). Salt tolerance of mango rootstock (Magnifera indica L. cv.Osteen). Spanish Journal of Agricultural Research, 1(1), 67-78.
  14. Esfandyari, A., Abbasi, A., Enayati, V. & Mousavi, S. (2010). Different behavior of root and leaf in grass pea landraces in response to oxidative stress caused by salinity. Journal of Agricultural Science and Sustainable Production, 2(4), 65-76. (in Farsi)
  15. Ferreira-Silva, S. L., Silveira, J., Voigt, E., Soares, L. & Viegas, R. (2008). Changes in physiological indicators associated with salt tolerance in two contrasting cashew rootstocks. Brazilian Journal of Plant Physiology, 20(1), 51-59.
  16. Fujita, T., Maggio, A., Rios, M. G., Stauffacher, C., Bressan, R. A. & Csonka, L. N.  (2003). Identification of regions of the tomato–glutamyl kinase that are involved in allosteric regulation by proline. Acta Hortticulturae, 278-288.
  17. Galeshi, S., Torabi, B., Resam, G. H., RahemiKarizaki, A. & Barzegar, A. (2009). Stress management in plants. Gorgan University of Agricultural Sciences and Natural Resources Press, 30, 23-29.
  18. Gaspar, T., Penel, C., Castillo, F. J. & Greppin, H. (1985). A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiology of Plants, 64, 418-423.
  19. Ghahremanzadeh, A., Piri, S. & Imani, A. (2015). Effects of salinity stress on some physiological properties of almond. Journal of Novel Applied Sciences, 12, 1246-1248.
  20. Gholami, M. & Rahemi, M. (2009). Effect of NaCl salt stress on physiological and morphological characteristic of vegetative peach- almond hybrid (GF677) rootstock. Plant Production Technology, 9(1), 173-181. (in Farsi)
  21. Gill, S. S. & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinary in abiotic stress tolerant in crop plants. Plant Physiology and Biochemistry, 48, 909-930.
  22. Grattan, S. R. & Grieve, C. M. (1999). Salinity-mineral nutrient relations in horticultural crops. Scientia Horticulturae, 78, 127-157.
  23. Harinasut, P., Poonsopa, D., Roengmongkol, K. & Charoensataporn, R. (2003). Salinity effects on antioxidant enzymes in mulberry cultivars, ScienceAsia, 29, 109-113.
  24. Heydari-Sharifabad, H. (2001). Plant and salinity. Forestry and Rangeland Research Institute, 76 pp. (in Farsi)
  25. Jaleel, C. A. (2009). Soil salinity regimes alters antioxidant enzyme activities in two varieties of Catharanthus roseus. Botany Research International, 2(2), 64-68.
  26. Kang, H.N. & Saltveit. V. (2002). Effect of chilling on antioxidant enzymes and DPPH-radical scavenging activity of high- and low-vigour cucumber seedling radicles. Plant, Cell and Environment, 25, 1233-1238.
  27. Karimi, S., Rahemi, M., Maftoun, M., Eshghi, S. & Tavallali, V. (2009). Effects of long-term salinity on growth and performance of two pistachio rootstocks. Australian Journal of Basic and Applied Sciences 3(3), 1630-1639.
  28. Kim, S. Y., Lim, J. H., Park, M. R., Kim, Y. J., Park, T. I., Seo, Y. W., Choi, K.G. & Yun, S. J. (2005). Enhancedantioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. Journal of Biochemistry and Molecular Biology, 38(2), 218-224.
  29. Manivannan, P., Jaleel, C. A., Sankar, B., Kishorekumar, A., Somasundaram, R., Lakshmanan, G. M. A. & Panneerselvam, R. (2007). Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids Surf B Biointerfaces, 59, 141-149.
  30. Mashayekhi, M., Amiri, M. E. & Habibi, F. (2015). Study of biochemical reactions and enzymatic activity of Gf677 rootstocks (peach-almond rootstock) in salt stress. Journal of Horticultural Science, 29(2), 207-215.
  31. Matsumoto, K., Tamura, F., Chun, J. & Tanabe, K. (2006). Native mediterranean Pyrus rootstock, P. amygdaliformis and P. elaeagrifolia, present higher tolerance to salinity stress compared with Asian natives. Journal of Japan Society Horticulture Science, 75(6), 450-457.
  32. Merati, M., Niknam, V., Hasanpour, H. & Mir Masoumi, M. (2015). Comparative effects of salt stress on growth andantioxidative responses in different organs of pennyroyal (Mentha pulegium L.). Journal of Plant Research, 28(5), 1097-1107. (in Farsi)
  33. Momenpour, A., Bakhshi, D., Imani, A. & Rezaie, H. (2015). Effect of salinity stress on nutrient concentrations in almond cultivars Shokoufa, Sahand and Genotype 13-40 on the GF677 Rootstock.  Journal of Horticultural Science, 29(2), 255-268. (in Farsi)
  34. Munns, R. & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681.
  35. Munns, R. (2002). Comparative physiology of salt and water stress. Plant Cell Environ, 25, 239-250.
  36. Noitsakis, B., Dimassi, K. & Therios, I. (1997). Effect of NaCl induced salinity on growth. Chemical composition and water relation of two almond (Prunus amygdalus L.) cultivars and the hybrid GF677. (Prunus amygdalus- Prunus persica). Acta Horticulturae, 449, 641-648.
  37. Olsen, S. R. & Sommers, L. E. (1982). Phosphorus. pp. 403-430, In: C.A. Black (ed.), Methods of Soil Analysis. The National Academy of Sciences, London.
  38. Oraei, M., Tabatabaei, S. J., Fallahi, E. & Imani, A. (2008). The effects of salinity stress and rootstock on the growth, photosynthetic rate, nutrient and sodium concentrations of almond (Prunus dulcis Mill). Journal of Horticultural Sciences, 10, 131-140. (in Farsi)
  39. Petrov, V. D. & Breusegem, F. V. (2012). Hydrogen peroxide-a central hub for information flow in plant cells. Cell Biology, 14, 1093-1206.
  40. Rahemi, M., Nagafian, S. H. & Tavallaie, V. (2008). Growth and chemical composition of hybrid GF677 influenced by salinity levels of irrigation water. Journal of Plant Sciences, 7(3), 309-313.
  41. Rahnomoon, H., Shekari, F., Dejampour, J. & Khorshidi, M. (2013). Effect of different salinity levels on some morphological and biochemical changes of almond (Prunus dulcis. Mill). Journal of Agriculture, 14(2), 179-192. (in Farsi)
  42. Reddy, Y. V. & Srivastava, G. C. (2003). Superoxide dismutase and peroxidase activities in ripening mango (Mangifera indica L.) fruits. Indian Journal of Plant Physiology, 8, 115-119.
  43. Rengel, Z. & Damon, P.M. (2008). Crops and genotypes differ in efficiency of potassium uptake and use. Physiologia Plantarum, 133, 624-636.
  44. Rengel, Z. & Marschner, P. (2005). Nutrient availability and management in the rhizosphere: exploiting genotypic differences. New Phytologist, 168, 305-312.
  45. Sadeghi, H. (2011). Differential response to salinity in two Iranian barley (Hordeum vulgare L.) cultivars. Romanian Agricultural Research, 28, 57-64.
  46. Sadri, H. & Imani, A. (2013). Investigating the effects of various concentrations of indole butyric acid (IBA) hormone and culture medium on rooting of GF677 rootstock cuttings. The first conference on the application of modern science and technology in agriculture and natural resources, 16-26. (in Farsi)
  47. Sainju, U. M., Dris, R. & Singh, B. (2003). Mineral nutrition of tomato. Food, Agriculture & Environment, 1(2), 176-183.
  48. Shani, U. & Ben-Gal, A. (2005). Long-term response of grape vines to salinity: osmotic effects and ion toxicity. American Journal of Enology and Viticulture, 56(2), 148-154.
  49. Smart, R. E. & Bingham, G. E. (1974). Rapid estimates of relative water content. Plant Physiology, 53, 258-260.
  50. Smirnoff, N. (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125, 27-58.
  51. Sotiropoulos, T. E. (2007). Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M4 cultured in vitro. Biologia Plantarum, 51, 177-180.
  52. Sotiropoulos, T. E., Therios, I. N., Almaliotis, D., Papadakis, I. & Dimassi, K. N. (2006). Response of cherry rootstocks to boron and salinity. Journal of Plant Nutrition, 29, 1691-1698.
  53. Sreenivasulu, N., Grimm, B., Wobus, U. & Weschke, W. (2000). Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt sensitive seedlings of foxtailmillet (Setaria italica). Journal of Plant Physiology, 109, 435-442.
  54. Szabados, L. & Arnould, S. (2009). Proline: a multifunctional amino. Plant Science, 15, 89-97.
  55. Waling, I., Vark, W. V., Houba, G. & Vanderlee, J. J. (1989). Soil and Plant Analysis. Wageningen Agriculture University, Netherland.
  56. Wang, C. (1995). Effect of temperature preconditioning on catalase, peroxidase, and superoxide dismutase in chilled zucchini squash. Postharvest Biology and Technology, 5, 67-76.
  57. White, P. J., Hammond, J. P., King, G. J., Bowen, H. C., Hayden, R. M., Meacham, M. C., Spracklen, W. P. & Broadley, W. R. (2010). Genetic analysis of potassium use efficiency in Brassica oleracea. Annals of Botany, 105, 1199-1210.
  58. Yamasaki, S. & Dillenburg, L.C. (1999). Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilian Fisiologia Vegetal, 11, 69-75.
  59. Yazici, I., Turkan, F., Sekmen, A. H. & Demiral, T. (2007). Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environmental Experimental Botny, 61(1), 49-57.
  60. Zarabi, M. M., Talayi, A., Soleymani, A. & Hadad, R. (2011). Physiological role and biochemichal changes of six olive cultivars in drought stress. Journal of Horticultural Science, 24(2), 234-244.
  61. Zhang, M., Fang, Y., Ji, Y., Jiang, Z. & Wang, L. (2013). Effects of salt stress on ion content, antioxidant enzymes and protein profile in different tissues of Broussonetia papyrifera. South African Journal of Botany, 85, 1-9.     
  62. Zhang, Z., Huber, D. & Rao, J. (2013). Antioxidant systems of ripening avocado (Persea americana Mill.) fruit following treatment at the preclimacteric stage with aqueous 1-methylcyclopropene. Postharvest Biology and Technology, 76, 58-64
  63. Zrig, A., Ben Mohamed, H., Tounekti, T., Ahmed, S. & Khemira, H. (2015). Differential responses of antioxidant enzymes in salt-stressed almond tree grown under sun and shade conditions. Plant Science and Research, 2(1), 23-30.
  64. Zrig, A., Tounekti, T., Vadel, A. M., BenMohamed, H., Valero, D., Serrano, M., Chtara, C. & Khemira, H. (2011). Possible involvement of polyphenols and polyamines in salt tolerance of almond rootstocks. Plant Physiology and Biochemistry, 49, 1313-1320.