Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effects of media cultures and plant growth regulators on micropropagation of CAB-6P cherry semi-dwarf rootstock

Document Type : Full Paper

Authors
Maliheh Fallahpour 1
seied mehdi miri 2
Naser Bouzari 3
1 Former Ph.D. Student, Faculty of Agriculture and Natural Resources, Mohaghegh Ardabili University, Ardabil, Iran
2 Associate Professor, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran
3 Associate Professor, Horticultural Science Research Institute, Temperate Fruits Research Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
10.22059/ijhs.2018.248845.1370
Abstract
CAB-6P is used as a semi dwarf rootstock for sweet and sour cherries. The purpose of this study was to determine the best media culture and plant growth regulators on micropropagation of CAB-6P rootstock. Shoot-tips were cultured on three culture media (MS, DKW and WPM) containing different concentrations of BAP (0.5, 1 and 2 mg/L) in combination with Kin (0 and 0.5 mg/L) for proliferation. Multiplied shoots were rooted on MS or WPM media supplemented with IBA (0, 0.5, 1 and 2 mg/L). Results showed that the most suitable multiplication medium was WPM+0.5 mg/L BA with 8.1 shoots per explant. The highest rooting percentage (69%) was obtained with MS+2 mg/L IBA. Well rooted plantlets were transferred to peat moss:cocopeate:perlite (2:2:1 v/v) substrate and successfully hardened (88%).
Keywords
Culture medium
multiplication
Prunus
rooting
Tissue culture
Subjects
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Iranian Journal of Horticultural Science
Volume 50, Issue 1
Spring 2019
Pages 187-196

  • Receive Date 20 January 2018
  • Revise Date 09 April 2018
  • Accept Date 28 April 2018