Effects of media cultures and plant growth regulators on micropropagation of CAB-6P cherry semi-dwarf rootstock

Document Type : Full Paper

Authors

1 Former Ph.D. Student, Faculty of Agriculture and Natural Resources, Mohaghegh Ardabili University, Ardabil, Iran

2 Associate Professor, Faculty of Agriculture, Karaj Branch, Islamic Azad University, Karaj, Iran

3 Associate Professor, Horticultural Science Research Institute, Temperate Fruits Research Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

CAB-6P is used as a semi dwarf rootstock for sweet and sour cherries. The purpose of this study was to determine the best media culture and plant growth regulators on micropropagation of CAB-6P rootstock. Shoot-tips were cultured on three culture media (MS, DKW and WPM) containing different concentrations of BAP (0.5, 1 and 2 mg/L) in combination with Kin (0 and 0.5 mg/L) for proliferation. Multiplied shoots were rooted on MS or WPM media supplemented with IBA (0, 0.5, 1 and 2 mg/L). Results showed that the most suitable multiplication medium was WPM+0.5 mg/L BA with 8.1 shoots per explant. The highest rooting percentage (69%) was obtained with MS+2 mg/L IBA. Well rooted plantlets were transferred to peat moss:cocopeate:perlite (2:2:1 v/v) substrate and successfully hardened (88%).

Keywords

Main Subjects


  1. Bell, R. L., Srinivasan, C. & Lomberk, D. (2009). Effect of nutrient media on axillary shoot proliferation and preconditioning for adventitious shoot regeneration of pears. In vitro Cellular and Developmental Biology-Plant, 45, 708-714.
  2. Buyukdemirci, H. (2008). The effects of medium ingredients on shoot propagation and rooting of cherry rootstocks in vitro. Acta Horticulturae, 795, 419-422.
  3. Dimassi-Theriou, K. (1995). In vitro rooting of rootstock GF 677 (Prunus amygdalus × P. persica) as influenced by mineral concentration of the nutrient medium and type of culture-tube sealing material. Journal of Horticultural Science, 70(1), 105-108.
  4. Erfani, M., Miri, S. M. & Imani, A. (2017).In vitro shoot proliferation and rooting of Garnem rootstock as influenced by basal media, plant growth regulators and carbon sources. Plant Cell Biotechnology and Molecular Biology, 18(3&4), 101-109.
  5. Erwin, D. & Ribeiro, O. (1996). Phytophthora diseases worldwide. American Phytopathological Society Press, USA.
  6. Fallahpour, M., Miri, S. M. & Bouzari, N. (2015). In vitro propagation of ‘Gisela 5’ rootstock as affected by mineral composition of media & plant growth regulators. Journal of Horticultural Research, 23 (1), 57-64.
  7. Fotopoulos, S. & Sotiropoulos, T. E. (2005). In vitro propagation of the PR 204/84 (Prunus persica × P. amygdalus) rootstock: Axillary shoot production and rhizogenesis. New Zealand Journal of Crop and Horticultural Science,33 (1), 75-79.
  8. Gangi Moghadam, E., Bolandi, A. R. & Anahid, S. (2008). Micropropagation of four selected dwarf mahaleb (Prunus mahaleb L.) genotypes. Pajouhesh-va-Sazandegi, 79, 54-61. (in Farsi)
  9. Glass A. D. M., Britto, D. T., Kaiser, B. N., Kinghorn, J. R., Kronzucker, H. J. & Kumar, A. (2002). The regulation of nitrate and ammonium transport systems in plants. Journal of Experimental Botany, 53(370), 855-864.
  10. Gyeviki, M. (2005). The effect of three rootstocks on yield and fruiting of sweet cherry. Acta Horticulturae, 667, 562-568.
  11. Hammatt, N. & Grant, N. G. (1993). Apparent rejuvenation of mature wild cherry during micropropagation. Journal of Plant Physiology, 141(3), 341-326.
  12. Hasanloo, T., Jafarkhani Kermani, M., Malmir Chegini, M., Sepehrifar, R., Mohajeri Naraghi, S. & Miri, S. M. (2014). Optimization of in vitro propagation of Qare-Qat (Vaccinium arctostaphylus). Journal of Medicinal Plants and By-products. 2: 21-26.
  13. Hirschi, K. D. (2004). The calcium conundrum. Both versa-tile nutrient & specific signal. Plant Physiology, 136(1), 2438-2442.
  14. Janekova, M. (1985). In vitro shoot growth from the buds of selected vegetatively propagated bird cherries (Cerasus avium). Zahradnictvi - UVTIZ, 12(3), 165-168.
  15. Kalinina, A. & Brown, D. C. W. (2007). Micropropagtion of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator. Plant Cell Reports, 26(7), 927-935.
  16. Karamad, Z., Gangi Moghadam, E. & Bolandi, A. R. (2014). Effects of culture media and growth regulators on micropropagation of Gisela 6 rootstock. Journal of Crops Improvement, 16(2),
    339-351. (in Farsi)
  17. Khosravinezhad, F., Abdollahi, H., Kashefi, B., Hassani, M. & Salehi, Z. (2016). Study on in vitro propagation of some promising quince (Cydonia oblonga) cultivars. Iranian Journal of Horticultural Science, 47(1), 135-144. (in Farsi)
  18. Long, L. E. & Kaiser, C. (2010). Sweet cherry rootstocks. A Pacific Northwest Extension Publication. OR: Oregon State University.
  19. Mahdavian, M., Bouzari, N. & Abdollahi, H. (2010). Effects of culture media and growth regulators on proliferation and rooting of a vegetative mahaleb rootstock (SL-64). Seed and Plant Improvement Journal, 26(1), 15-26. (in Farsi)
  20. Mahdavian, M., Bouzari, N. & Abdollahi, H. (2011). Effects of media and plant growth regulators on micropropagation of a dwarfing cherry rootstock (PHL-A). Biharean Biologist, 5(2), 86-90.
  21. Mansseri-Lamrioui, A., Louerguioui, A., Bonaly, J., Yakoub-Bougdal, S., Allili, N. & Gana-Kebbouche, S. (2011). Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration. African Journal of Biotechnology, 10(43), 8613-8624.
  22. Mihovilović Bošnjak, A., Kereša, S., Habuš Jerčić, I. & Barić, M. (2012). The effect of cytokinin type and explant orientation on axillary shoot proliferation and in vitro rooting of Gisela 5 cherry rootstock. Journal of Food Agriculture & Environment, 10(3&4), 616-620.
  23. Miri, S. M. (2018). Effect of IAA, IBA and NAA auxins on in vitro rooting of M.9 and M.26 apple. Cellular and Molecular Plant Biology Journal, 12(4), 15-23. (in Farsi)
  24. Miri, S. M., Vaez Livari, B., Khalighi, A. & Ghaem Maghami, S. A. )2003a(. Effect of carbohydrate, gibberellic acid, indolebutyric acid, phloroglucinol, explant orientation and culture vessel volume on optimizing in vitro propagation of M.9 apple rootstock. Pajouhesh-va-Sazandegi, 16(2), 31-37. (in Farsi)
  25. Miri, S. M., Vaez Livari, B., Khalighi, A. & Ghaem Maghami, S. A. (2003b). Phenolic oxidation reduction and in vitro proliferation of shoots of apple clones ‘M.9’ and ‘M.26’. Iranian Journal of Horticultural Science and Technology, 4(1&2), 145-154. (in Farsi)
  26. Mousavi, S. S., Miri, S. M. & Moradi, P. (2017). Optimization of micropropagation of jujube (Ziziphus jujuba cv. Tian-yuzao). Journal of Agronomy and Plant Breeding, 13(4), 1-11. (in Farsi)
  27. Pierik R. L. M. (1997). In vitro culture of higher plants. Springer Science and Business Media, Springer, Dordrecht, the Netherlands.
  28. Pruski, K., Astatkie, T. & Nowak, J. (2005). Tissue culture propagation of Mongolian cherry (Prunus fruticosa) and Nanking cherry (P. tomentosa). Plant Cell, Tissue and Organ Culture, 82, 207-211.
  29. Pruski, K., Lewis, T., Astatkie, T. & Nowak, J. (2000). Micropropagation of Chokecherry and Pincherry cultivars. Plant Cell, Tissue and Organ Culture, 63, 93-100.
  30. Reddy, A. S. N. (2001). Calcium: Silver bullet in signaling. Plant Science, 160(3), 381-404.
  31. Sarropoulou, V., Dimassi-Theriou, K. & Therios, L. (2013). In vitro rooting and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6 using L-methionine. Turkish Journal of Agriculture and Forestry, 37, 688-698.
  32. Sarropoulou, V., Dimassi-Theriou, K. & Therios, L. (2014a). Dikegulac-sodium effect on micropropagation and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6. Turkish Journal of Agricultural and Natural Sciences, 2, 1713-1726.
  33. Sarropoulou, V., Dimassi-Theriou, K. & Therios, L. (2014b). Ιn vitro plant regeneration from leaf explants of the cherry rootstocks CAB-6P, Gisela 6, and M×M 14 using sodium nitroprusside.  In Vitro Cellular & Developmental Biology-Plant, 50(2), 226-234.
  34. Sarropoulou, V., Dimassi-Theriou, K. & Therios, L. (2015). Medium strength in inorganics and PVP concentration effects on cherry rootstocks in vitro rooting. Horticultural Science, 42(4), 15-192.
  35. Sarropoulou, V., Dimassi-Theriou, K. & Therios, L. (2017). Effects of the exogenous polyamines on micropropagation of cherry rootstocks. Indian Journal of Plant Physiology, 22(2), 227-239.
  36. Shi, D. (2014). Effects of culture media and plant growth regulators on micropropagation of willow (Salix matsudana ‘Golden Spiral’) and hazelnut (Corylus colurna ‘Te Terra Red). M.Sc. thesis of Horticulture, University of Nebraska.
  37. Sisko, M. (2011). In vitro propagation of Gisela 5 (Prunus cerasus × P. canescens). Agricultura, 8, 31-34.
  38. Tatari Varnousfaderani, M., Mousavi, S. A. & Bouzari, N. (2012). Micropropagation of some clonal rootstocks of stone fruits. Seed and Plant Improvement Journal, 28-1(1), 53-66. (in Farsi)
  39. Zarei, M., Garoosi, Gh., Nezami, E., Hosseini, R. & Ahmadi, J. (2013). The effect of medium, carbon source, light spectrum and style treatment of auxin on shoot and root regeneration of Gisela 6 root stock. Journal of Cell and Tissue, 4(2), 169-185. (in Farsi)
  40. Zhang, Z., Dai, W. H., Cheng, Z. M. & Walla, J. A. (2000). A shoot-tip culture micropropagation system for chokecherry. Journal of Environmental Horticulture, 18 (2), 234-237.