Effects of Nano silver on bacterial contamination and morphological and biochemical indices of in vitro GN15 almond rootstock

Document Type : Full Paper


1 Former Ph.D. Student, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

2 Associate Professor, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

3 Associate Professor, Faculty of agriculture, Urmia University, Urmia, Iran


Nanotechnology has been able to pave the way for new methods in plant sciences and agricultural research. This research was carried out to investigate effects of Nanosilver in 4 concentrations (0 (control), 100, 150 and 200 mg L-1) at 8 replications in a complete randomized block design, on viability, bacterial contamination and some morphological and biochemical indices of GN15 explants at Urmia University. Shoot tip of cultivated GN15 rootstock were placed in MS medium containing 3% sucrose and 0.8% agar and different concentrations of Nanosilver and 1 mg L-1 BAP. Samples were recultured and were grown in growth chamber. By increasing Nanosilver concentration, the plants survival increased and the percentage of bacterial and fungal contamination decreased. Nanosilver increased the root length, leaf number, chlorophyll a, b, total chlorophyll, carotenoids and soluble carbohydrates at concentrations from 0 to 100 mg L-1. At higher concentrations, these factors were decreased. Increasing the concentration of Nanosilver decreased the shoot number and lateral branches number of explants, but did not have a significant effect on root number. Despite the effect of increasing the concentration of Nano silver on reducing bacterial contamination, up to 200 mg L-1, even to less than 10%, the best level, improving the biotic properties of GN15 explants was 100 mg L-1.


Main Subjects

  1. Abdi, G. (2012). Evaluation the potential of Nano silver for removal of bacterial contaminations in valerian (Valeriana officinalis L.) tissue culture. Journal of Biology and Environmental Science, 6(17), 199-205.
  2. Abdi, G., Salehi, H. & Khosh-Khui, M. (2008). Nano silver a novel Nano material for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture. Acta Physiologiae Plantarum, 30(5), 709-714.
  3. Aghajani, Z., Optional, R. & Pourmadidin, A. (2014). Investigating the effects of silver nanoparticles on germination and early growth of Thymus kotscgyanus L. Agronomic Research in the Margin of the Desert, 11(2), 73-81. (in Farsi)
  4. Aghdaei, M., Salehi, H. & Sarmast, M. K. (2012). Effects of silver nanoparticles on Tecomella undulata (Roxb.) Seem. micro propagation. Advances in Horticultural Science, 26(1), 21-24.
  5. Amirghasemi, D. (2004). Grapes (planting, harvesting and processing). Tehran, Ayandegan publications. (in Farsi)
  6. Arifa, S., Subramanim, S., Laeson, T., Aristunenko, D., Colbeck, L. & Demidchik, V. (2012). Nano particle inhibit root and leaf growth, induce generation of reactive oxygen species and trigger elevation of cytosolic calcium in Arabidopsis thaliana. In: Proceedings of Abstracts of Plant Biology Congress, 29 Jul–3 Aug., Albert-Ludwig’s-University, Freiburg, Germany, pp: 296.
  7. Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23(1), 112-121.      
  8. Bhojwani, S. S. & Razdan, M. K. (1996). Studies in plant science, plant tissue culture: theory and practice. Elsevier Science Publishers.
  9. Cassells, A. C. (1991). Problems in tissue culture: culture contamination. In: P. C. Debergh & R. H. Zimmerman (Eds.), Micro propagation, technology and application. (pp. 31-44.) Dordrecht: Kluwer Academic.
  10. Chen, X. & Schluensener, H. J. (2008). Nano silver: a Nano product in medical application. Toxicology Letters, 176(2), 1-12.
  11. Daryani, P., Zare, N., Chamani, A. & JavadiMajd, D. (2015). The effect of silver Nano particles on microbial contamination and in vitro growth of apical and axillary buds of hazelnut cultivars. Biotechnology in Agriculture, 14(1), 21-31. (in Farsi)  
  12. Delvin, M. R. & Withman, F. H. (2002). Plant Physiology. Delhi, CBs publishers and distributers, Chapter 12.
  13. Dibrov, P., Dzioba, J., Gosnik, K. K. & Hase, C. C. (2002). Chemiosmosis mechanism of antimicrobial activity of Ag+ in Vibrio cholera. Antimicrobial Agents Chemotherapy, 46(8), 2668-2670.
  14. Ehsanpour, A. A. & Nejati, Z. (2013). Effect of Nano silver on potato plant growth and protoplast viability. Biological Letters, 50(1), 35-43.
  15. Ekhtiari, R., Mohebbi, H. R. & Mansouri, M. (2011). Investigation of Nano silver particles on salinity tolerance of Foeniculum vulgare Mill. in early growth in laboratory conditions. Journal of Plant and Biological Research, 7(27), 62-55. (in Farsi)
  16. Felipe, A. J. (2009). Felinem, Garnem and Monegro almond *peach hybrid rootstocks. HortScience, 44(1), 196-197.
  17. Fideghelli, C., Strada, G. D., Grassi, F. & Morico, G. (1998). The peach industry in the world: present situation and trend. In: Proceedings of IV international Peach symposium, Acta Horticulture, pp: 465. 
  18. Ghasemi Pirbaloti, A. (2010). Medicinal and aromatic plants (identifying and studying their effects). Islamic Azad University Press, Shahr-e-Kord. (in Farsi)
  19. Giridhar, P., Indu, E. P., Vijayarama, D. & Ravishankar, G. A. (2003) Effect of silver nitrate on in vitro shoot growth of coffee. Tropical Science, 43(3), 144-146.                                           
  20. Hatami, M. & Ghorbanpour, M. (2014). Defense enzyme activities and biochemical variations of Pelargonium zonal in response to Nano silver application and dark storage. Turkish Journal of Biology, 38(1), 130-139.
  21. Irigoyen, J. J., Emerich, D. W. & Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of praline and total soluble sugars in modulated alfalfa (Medicago sativa) plants. Plant Physiol, 84(1), 55-60.  
  22. Jasim, B., Roshmi, T., Juothis, M. & Radhakishna, E. K. (2017). Plant growth and dysgenic enhancement effect of silver nanoparticles in Fenugreek (Trigonella foenumgraecum L.). Saudi Pharm, 25(3), 443-447.
  23. Khodakovskaya, M., Dervishi, E., Mahmood, M., Xu, Y., Li, Z., Watanabe, F. & Biris, A. S. (2009). Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano, 3(10), 3221-3227.
  24. Leifert, C. & Casselles, A. C. (2001). Microbial hazards in plant tissue and cell cultures. In Vitro Cellular and Developmental Biology-Plant, 37(2), 133-138.
  25. Lubick, N. (2008). Nano silver toxicity: Ions, Nano particles or both? Environmental Science and Technology, 42(23), 8617-8617.
  26. Mahajan, Sh. & Tuteja, N. (2005). Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics, 444(2), 139-158.
  27. Mahna, N., Zununivahed, S. & Khani, S. (2013). Plant in vitro culture goes Nano: Nano silver mediated decontamination of ex vitro explants. Nano Medicine and Nanotechnology, 4(2), 1-4.
  28. Nazari Moghaddam Aghayi, R., Yadollahi, A., Moeini, A. & Sepahvand, S. (2013). In vitro culture of Gisela 6 semi-dwarf rootstock. Journal of Biology and Environmental Science, 7(20), 57-64.
  29. Nowroozi, M., Tedin, M. R. & Nowruz, S. (2016). Induction of oxidative stress in barley seedlings (Hordeum vulgare L.) in response to silver nanoparticles. Journal of Crops and Environmental Stresses, 1(1), 11-19. (in Farsi)
  30. Perl, A., Aviv, D. & Galun, E. (1988). Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of alien gene. Plant Cell Reports, 7(6), 403-406.
  31. Rezvani, N. & Sorooshzadeh, A. (2014). Effect of Nano silver on root and bud growth of saffron in flooding stress condition. Saffron Agriculture and Technology, 2(1), 91-104. (in Farsi)
  32. Rezvani, N., Sorooshzadeh, A. & Farhadi, N. (2012). Effect of Nano silver on growth of saffron in flooding stress. World Academy of Science, Engineering and Technology, 6(1), 517-522.
  33. Roofigari Isfahan, M., Doudi, M. & Rezayatmand, Z. (2014). Anticandidal effects of aqueous and callus extracts of Artemisia aucheri. International Journal of Molecular and Clinical Microbiology, 1(1), 389-397.
  34. Rostami, A. A. & Shahsavar, A. (2009). Nano-silver particles eliminate the in vitro contaminations of olive Mission explants. Asian Journal of Plant Science, 8(2), 505-509.
  35. Safavi, K. (2014). Effect of titanium dioxide Nano particles in plant tissue culture media for enhance Resistance to bacterial activity. Bulletin of Environment, Pharmacology and Life science, 3(5), 163-166.
  36. Scrinis, G. & Lyons, K. (2007). The emerging Nano-corporate paradigm: Nano technology and the transformation of nature, food and agro-food systems. International Journal Sociology of Food and Agriculture, 15(2), 22-44.
  37. Shokri, S., Babaie, A. R. & Ahmadian Arab, M. M. (2014). The effects of different concentrations of nano-silver on elimination of bacterial contaminations and phenolic exudation of Rose (Rose hybrid L.) in vitro culture. International Journal of Farming and Applied Sciences, 1(3), 50-54. 
  38. Sondi, I. & Salopek-Sondi, B. (2004). Silver Nano particles as antimicrobial agent: case study on E. coli as a model for gram e negative bacteria. Journal of Colloid and Interface Science, 275(1), 177-182.
  39. Tabatabaei, Z., Razavi-zadeh, R. & Rostami, F. (2013). Effect of nanoparticles on chlorophyll, carotenoid and flavonoids in Brassica napus in in vitro condition. In: Proceedings of 2nd National Conference on Nanotechnology from Theory to Application, 1 March, Aseman Hotel, Isfahan, Iran, pp: 1-10. (in Farsi)
  40. Taghizadeh, M. & Solgi, M. (2014). The application of essential oils and silver Nano particles for sterilization of Bermuda grass explants in in vitro culture. International Journal of Horticultural Science and Technology, 1(2), 131-140.
  41. Turhan, H. (2004). The effect of silver nitrate (ethylene inhibitor) on in vitro shoot development in potato (Salanum tuberosum L.). Biotechnology, 3(1), 72-74.
  42. Vankar, P. S. & Shukla, D. (2012). Biosynthesis of silver nano particles using lemon leaves extract and its applications for antimicrobial finish on fabric. Applied Nanoscience, 2(2), 163-168.
  43. Yin, L., Cheng, Y., Espinasse, B., Colman, B. P., Auffan, M., Wiesner, M., Rose, J., Liu, J. & Bernhardt, E. S. (2011). More than the ions: the effects of silver nanoparticles on Lolium multiflurum. Environmental Science and Technology, 45(6), 2360-2367.
  44. Yusefzaiy, F, Pourakbar, L. & Farhadi, Kh. (2015). Effect of Nano silver on some morphological and physiological indices of Basil plant. Iranian Journal of Plant Physiology and Biochemistry, 1(2), 63-73. (in Farsi)