Study of Interaction effect of culture media and growth regulator concentrations on Micro propagation of pear vigorous rootstock (Pyrus betulifolia)

Document Type : Full Paper


1 Former M.Sc. Student, Faculty of Agirculture, Ilam University, Iran

2 Professor Associate of Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Assistant Professor, Faculty of Agirculture, Ilam University, Iran

4 Associate Professor, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran


In order to achieve maximum proliferation in culture media, different amounts of salts and growth regulators could be used regarding to plant species. Sometimes, interaction effects of these materials could results in different results on explant’s proliferation in vitro condition. In the present study the effect of two basic culture media including MS and QL on Micro propagation of Pyrus betulifolia (pear vigorous rootstock) were studied. The highest amount of proliferation was achieved in QL culture medium and highest amount leaf expansion was in MS culture medium. In order to produce more leaf expansion in QL culture medium and as for existence of difference in ions pure amount including NO3-, NH4+, Cl-and Ca2+ in two culture media, NH4NO3 in three concentrations including 6.25, 12.5 and 18.75 mM and CaCl2 in two concentrations including 0.9 and 1.8 mM were added to QL culture medium. As for the result, increasing NH4NO3 and CaCl2 amounts had no effect on proliferation of explants but by increasing NH4NO3 and CaCl2 leaf expansion increased in QL culture medium. In the other experiment for increasing of proliferation rate in MS culture medium, by increasing BAP amount, from 0.5 to 2 mg/l in MS culture medium, proliferation in this medium was increased. Apparently in MS culture medium with more concentration of macro elements compared to QL culture medium, it needs to use more growth regulators for achieving suitable proliferation. Totally, Results of this study showed that to succeed micro propagation of Pyrus betulifolia in both two basic culture media requires suitable concentrations of BAP, NH4NO3 and CaCl2.


Main Subjects

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