Investigation of polymorphism in different acid lime (Citrus aurantifolia Swingle) genotypes with free witches broom disease by molecular markers of SSR and ISSR

Document Type : Full Paper

Authors

1 Former Ph. D. Student, Faculty of Agriculture, University of Guilan, Rasht, Iran

2 Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran

3 Associate Professor, Citrus and Subtropical Fruits Research Center, Ramsar, Iran

4 Assistant Professor, Citrus and Subtropical Fruits Research Center, Ramsar, Iran

5 Assistant Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran

Abstract

Limes have been consumed as fresh fruits and processing products, due to high quality and acidity in fruit juice. In recent years, the incidence of Witches Broom disease resulted in destroy of thousands Limes trees in the south of Iran. The best method for controlling this disease is planting tolerant genotypes. Therefore, in this study, genetic diversity of 33 different lime genotypes which didn’t showed Witches Broom disease in the field and preliminary test, with 10 commercial citrus cultivars as a control was evaluated by 17 pair primers of SSR and 11 primers of ISSR. According to results with Simple Sequence Repeat (SSR) markers, the highest Polymorphic Information Content (PIC) were observed in TAA1 and TC26 with 0.72 and 0.68, respectively and by using ISSR markers were in ISSR1, 809 and 810 with 0.49 which was identified as a the most appropriate distinguishing markers. Results showed that there were differences between Limes and other types of citrus based on kind of studied markers. On the other hand, all of Lime genotypes had high affinity with Mexican Lime and Persian Lime and grouped in a cluster. Therefore, most of acid limes in south of Iran belonged to Limes group.

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References

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Iranian Journal of Horticultural Science
Volume 50, Issue 1
June 2019
Pages 1-11

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History

  • Receive Date: 25 September 2016
  • Revise Date: 29 November 2016
  • Accept Date: 03 March 2017

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