The effect of seed priming on biochemical characteristics of Borage (Borago officinalis) transplant in salt stress condition

Document Type : short paper


1 Former M. Sc. Student, College of Agriculture Science, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Associate Professor, College of Agriculture Science, Saveh Branch, Islamic Azad University, Saveh, Iran

3 Assistante Professor, College of Agriculture Science, Karaj Branch, Islamic Azad University, Karaj, Iran


Salinity stress, is one of the environmental stresses that has negative effects on seed germination and plant establishment in a soil and reduced the quantitive and qualitative performance indices of plants. For this purpose, salinity in four levels (1, 3, 6, and 9 ds/m) and priming in six levels – including no-priming, hydropriming, ascorbic acid in 10 and 20 mg/l and humic acid in 100 and 200 ml/l – were used. This experiment carried out in a factorial test based on completely randomized design with three replications. Results demonstrated that studied traits were significantly influenced by priming treatments. In the treatment of 20 mg of ascorbic acid, proline content (32.24 micrograms per gram), catalase activity (2.94 mol mg protein), and superoxide dismutase activity (0.28 U mg protein) were observed. The treatment of humic acid (200 ml/l) increased chlorophyll content to 0.57 mg/gFw. The treatment of ascorbic acid in 10 mg/l in extreme conditions of salinity (9 ds/m) showed the highest rate of peroxidase (5.34U/mg). In this experiment, the treatment of hydropriming had not acceptable efficiency in all traits.


Main Subjects

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