Evaluation some aspects of growth, physiological, yield and quality of watermelon Charleston Gray cultivar grafted and un-grafted under salt stress conditions

Document Type : Full Paper


1 Assistant Professor of Horticulture Science, Department of Horticulture and landscaping, Faculty of Agriculture, University of Zabol, Iran

2 Fromer Ph. D. Student, Department of Horticultural Science, Faculty of Agriculture, University of Guilan, Iran

3 Assistant Professor, Department of Horticulture, Faculty of Agriculture, Lorestan University, Iran

4 Laboratory Expert of Horticulture Science, Department of Horticulture, Faculty of Agriculture, Lorestan University, Iran

5 Assistant Professor of Horticultural Sciences Engineering, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran


Salinity is an important factor in limiting production in many countries. Today with using grafting methodinvegetable production could overcome problem of soil and irrigation water salinity resulted in production of high quality crops. In this study, water melon (cv. Charleston Gray) was grafted onto rootstocks including; Shintozwa, Buttle gourd, pepo, Bitter cucumber, Indigenous masses of Kermanshah and Hamedan watermelon with using hole insertion grafting method and grown under greenhouse and farm conditions in Faculty of Agriculture, Lorestan University in 2016. Grafted and un-grafted (control) plants were studied for some vegetative and biochemical traits after exposing to 0, 30 and 60 mM Na Cl. Results showed that there was a significant difference between the three levels of salinity treatment at the 5% probability level for leaf carotenoids. In this research, some of the vegetative traits such as length of main branch (220 cm), fresh weight of shoot (420 g) and root fresh weight (30 g) in plants that grafted onto Shintozwa rootstock in all three saline treatments were higher than non-grafted plant that were  102 cm, 225 and 10 g respectively. The highest (28467 kg ha-1) and lowest (10496 kg ha-1) total yield of fruit were related to the watermelon grafted onto Shintozwa rootstock and control plants respectively. Also, Shintozwa, Buttle gourd, Kermanshah and Hamedan watermelon rootstocks were a statistical group in terms of total yield. Thus, Shintozwa rootstock could be a suitable rootstock for water melon Charleston Gray cultivar under saline conditions.


Main Subjects

  1. Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Journal Agronomy, 23, 112-121.
  2. Ashouri, M., Hassanpour, A. & Zakerian, A. (2008). Investigating the effect of graft type on growth and yield of watermelon on cucurbit rootstock. Journal of Horticulture Science, 32, 31-40. (in Farsi)
  3. Castrillo, M. & Calcargo, A. M. (1998). Effects of water stress and rewatering on ribulose-1,5-bisphosphate carboxylase activity, chlorophyll and protein contents in two cultivars of tomato. Journal of Horticultural Science, 64, 717-724.
  4. Cohen, R., Burger, Y., Horev, C., Koren, A. & Edelstein, M. (2007). Introducing grafted cucurbits to modern agriculture.The Israeli Experience. Plant Disease, 91(3), 916-923.
  5. Colla, G., Rouphael, Y., Cardarelli, M., Massa, D., Salerno, A. & Rea, E. (2006).Yield, fruit quality and mineral composition of grafted melon plants grown under saline conditions. Journal of Horticultural Science and Biotechnology, 81(1), 146-152.
  6. Colla, G., Cardarelli, M., Fiorillo, A., Rouphael, Y. & Rea, E. (2012). Enhancing nitrogen use efficiency in cucurbitaceae crops by grafting. Proc.1S on GreenSys 2011.
  7. Colla, G. (2014).Vegetable grafting for abiotic stress tolerance: current status and advances through the cost action fa1204. In: Proceedings of the First International Symposium on Vegetables Grafting, Wuhan, China, 17-21 March 2014.
  8. Darryl, D. (2007). Nutrient management for cucurbits: melons, pumpkin, cucumber and squash. Department of crop and soil sciences. Michigan State University.
  9. Davis, A. R., Perkins-Veazie, P., Sakata, Y., Lopez-Galarza, S., Maroto, J. V., Lee, S. G., Huh, Y. C., Sun, Z., Miguel, A., King, S. R., Cohen, R. & Lee, J. M. (2008). Cucurbit grafting. Critical Review in Plant Sciences, 27, 50-74.
  10. Dettori, S. (1985). Leaf water potential, stomatal resistance and transpiration response to different watering in almond, peach and pixy plum. II International symposium on irrigation of horticultural crops. Acta Horticulturae, 171, 253-258.
  11. Edelstein, M. & Ben-Hur, M. (2014). Grafting: a useful tool to increase tolerance to toxic elements in vegetables under arid and semiarid condition. In: Proceedings of the First International Symposium on Vegetables Grafting, Wuhan, China, 17-21 March 2014.
  12. Edelstein, M., Ben-Hur, M., Ju-Young, Y., Bernstein, N., Nasser, A., Baumkoler, F. & Gerstl, Z. (2015). Preventing entry of pharmaceuticals (carbamazepine) into the food chain by using grafting plants. In: V International Symposium on Cucurbits. Cartagena, Murcia, Spain.
  13. Edelstein M., Cohen R., Baumkoler F., Ben-Hur M. (2016). Using grafted vegetables to increase tolerance to salt and toxic elements. Israel Journal of Plant Sciences, 1-18.
  14. FAOSTAT. (2015). 23 June 2015. http://faostat.fao.org/site/340/default.aspx.
  15. Gonzales, P. R. & Salas, M. L. (1995). Improvement of the growth, grain yield, and nitrogen, phosphorus and potassium nutrition of grain corn through weed control.Plant Nutrition, 18, 3313-3324.
  16. Gonzalea, L. & Gonzalez-Vilar, M. (2003). Determination of Relative Water Content.In Handbook of plant ecophysiology techniques, 207-212. (Eds J. Manuel and R. Goger). London: Kluwer Academic Publishers.
  17. Goreta, S., Bucevic-Popovic, V., Selak, G. V., Pavela-Vrancic, M. & Perica, S. (2008).Vegetative growth, superoxide dismutase activity and ion concentration of saltstressed watermelon as influenced by rootstock. Journal of Horticultural Science, 146, 695-704.
  18. Hejazi, A., Shahrudi, M. & Ardforush, M. (2007). Index method of plant analiyes. (7th ed.). (pp.197-234). (in Persian)
  19. Huang, Y., Zhu, J., Zhen, A., Liu, Z., Lei, B., Niu, M., Xie, J., Sun, J., Cao, H. & Bie, Z. (2015). Effectiveness and mechanism of rootstock grafting to increase cucumber salt tolerance. In: V International Symposium on Cucurbits. Cartagena, Murcia, Spain.
  20. Jiang, Y. & Huang, N. (2001). Drought and heat stress injury to two cool-season turf grasses in relation to antioxidation metabolism and lipid peroxidation. Crop Science, 41, 436-442.
  21. Kato, T. & Sasaya, S. (1986). Effect of rootstockon the yield mineral nutrition in cucumber. Robotics and Mechatronics, 11, 213-219.
  22. Khad, N (2004). Effects of irrigation, mulch, nitrogen and potassium on fruit yield and economics of watermelon.Journal of Maharashtra Agricultural Universities, 20 (1), 40-43.
  23. Lawlor, D. W. & Cornic, G. (2002). Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, Cell and Environment, 25, 275-294.
  24. Lee, J. M. (1994). Cultivation of grafted vegetables 1. Current status, grafting methods, and benefits, HortScience, 29, 235-239.
  25. Lee, J. M. & Oda, M. (2003) .Grafting of herbaceous vegetable and ornamental crops. Horticultural Reviews, 28, 61-124.
  26. Madhava Roa, K. V., Raghavendra, A. S. & Janardhan Reddy, K. (2006). Physiology and molecular biology of stress tolerance in plants (Eds.),15-39. Springer, printed in Netherland.
  27. Martins, A. L. C., Batagha, O. C., Camargo, O. A. & Contarella, H. )2003(. Corn yield and uptake of Cu, Fe, Mn and Zn from sewage sludge-amend soil with and without liming. Revista-Basilica Deciencia, 27, 563-574.
  28. Mostofi, Y. & Najafi, F. (2005). Laboratory manual of analytical techniques in horticulture. (Translation). Tehran University Press. Page 85. (in Farsi)
  29. Ozlem, A., Ozdemir, N. & Gunen, Y. (2007). Effect of grafting on watermelon plant growth, yield and quallity.Journal of Agronomy, 6, 362-365.
  30. Proietti, S., Rouphael, Y., Colla, G. & Battistelli, A. (2008). Fruit yield of mini- watermelon as affected by grafting and irrigation regimes. Journal of Science of Food and Agriculture, 88(6), 1107-1114.
  31. Rivero, R. M., Ruiz, J.M. &Romero, L. (2003). Role of grafting in horticultural plants under stress conditions. Food, Agriculture and Environment, 1, 70-74.
  32. Romero, L., Belakbir, A., Ragala, L. & Ruiz, M. J. (1997). Response of plantyield and leaf pigments to saline conditions: effectiveness of different rootstocks in melon plant (Cucumis melo L.). Soil Science and Plant Nutrition, 43, 855-862.
  33. Saberi, M. H., Zolfagharan, A., Nasrabad, A. A. & Atarodi, B. (2006). Effect of salinity on yield and yield componenets of watermelon cultivars. Seed and Plant Production, 22, 103-111.
  34. Saied, A. S., Keutgen, N. & Noga, G., (2003). Effects of NaCl stress on leaf growth, photosynthesis and ionic contents of strawberry cvs ‘Elsanta’ and ‘Korona’. In: Pardossi, A., Serra, G., Tognoni, F. (Eds.), International Symposium on Managing Greenhouse Crops in Saline Environment. International Society of Horticultural Science, Pisa, pp. 67-73.
  35. Staples, R. C. & Toenniessen, G. H. (1984). Salinity tolerance in plants. John Wiley and Sons. Pp: 443.
  36. Yang, Y., Wang, L., Tian, J., Li, J., Sun, J. & He, L. (2012). Proteomic studyparticipating the enhancement of growth and salt tolerance of bottle gourd rootstock grafted watermelon seedlings. Plant Physiology and Biochemistry, 58, 54-65.
  37. Yanjuan, Y., Li, Y., Wanga, L. & Guoa, S. (2015). Bottle gourd rootstock-grafting promotes photosynthesis by regulating the stomata and non-stomata performances in leaves of watermelon seedlings under NaCl stress. Journal of Plant Physiology, 186-187, 50-58.
  38. Yetisir, H. & Uygur, V. (2010). Responses of grafted water melon on to different gourd species to salinity stress. Plant Nutrition, 33, 315-327.