Factors affecting successful ex vitro microgragfting of sour lime (Citrus aurantifolia) in order to rescue rootless shoots

Document Type : Full Paper

Authors

1 Former Ph. D. Student, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

2 Associate Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

3 Assistant Professor, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

4 Assistant Professor, Shahid Beheshti University, Tehran, Iran

5 Associate Professor, Shahid Beheshti University, Tehran, Iran

6 Associate Professor, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

7 Assistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Abstract

This study was carried out to evaluate factors affecting ex vitro micrografting of sour lime (Citrus aurantifolia L.) such as size and type of scions, grafting type, micrografted plantlets conditions and usage of nutrient medium, in order to rescue rootless shoots developed form tissue culture or genetically transformed shoots. Our results showed that the optimum efficiency was achieved when bigger and woodier scions were used in conventional grafting method under controlled environmental conditions. Furthermore, soluble nutrients injected into the micrograft did not show significant difference compared with the control.  However, the number of successfully ex vitro micrografted explants treated with MS medium containing agar was higher than the parafilm covered micrografted explants. MS medium supplemented with agar can act as an additional nutritional source to the micrografted explants during the early days of micrografting. Moreover, it gives better anchorage and humidity support for scions and helps with the gradual acclimatization of them in in vivo conditions.

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References

  1. Aleza, P., Juárez, J., Ollitrault, P. & Navarro, L. (2009). Production of tetraploid plants of non apomictic citrus genotypes. Plant cell reports, 28(12), 1837-1846.
  2. Cantos, M., Ales, G. & Troncoso, A. (1993). Morphological and anatomical aspects of a cleft micrografting of grape explants in vitro. ISHS Acta Horticulturae, 388, 135-140.
  3. De Pasquale, F., Giuffrida, S. & Carimi, F. (1999). Minigrafting of shoots, roots, inverted roots, and somatic embryos for rescue of in vitro Citrus regenerants. Journal of the American Society for Horticultural Science, 124(2), 152-157.
  4. Dutt, M. & Grosser, J. W. (2009). Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus. Plant Cell, Tissue and Organ Culture, 98(3), 331-340.
  5. Dutt, M. & Grosser, J. W. (2010). An embryogenic suspension cell culture system for Agrobacterium- mediated transformation of citrus. Plant Cell Reports, 29(11), 1251-126.
  6. Dutt, M., Orbovic, V. & Grosser, J. W. (2009). Cultivar dependent gene transfer into citrus using Agrobacterium. In: Proceedings of the Florida State Horticultural Society, 122, 85-89.
  7. Dutt, M., Vasconcellos, M. and Grosser, J. (2011). Effects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime(Citrus aurantifolia L. Swingle). Plant Cell, Tissue and Organ Culture, 107(1), 79-8.
  8. Hassanen, S. A. (2013). In vitro grafting of pear (Pyrus spp.). World Applied Sciences Journal, 21(5), 705-709.
  9. Huang, S. C. & Millikan, D. (1980). In vitro micrografting of apple shoot tips, HortScience, 15(6), 741-743.
  10. Hussain, G. M., Wani, M., Rather, M. Z. & Bhat, K. (2014). Micrografting for fruit crop improvement. African Journal of Biotechnology, 13(25), 2474-2483.
  11. Jonard, R., Hugard, J., Macheix, J., Martinez, J., Mosella-Chancel, L., Poessel, J. L. & Villemur, P. (1983). In vitro micrografting and its applications to fruit science. Scientia Horticulturae, 20(2), 147-159.
  12. Jonard, R., Lukman, D., Schall, F. & Villemur, P. (1990). Early testing of graft incompatibilities in apricot and lemon trees using in vitro techniques. Scientia horticulturae, 43(1), 117-128.
  13. Marques, N. T., Nolasco, G. B. & Leitão, J. P. (2011). Factors affecting in vitro adventitious shoot formation on internode explants of Citrusaurantium L. cv. Brazilian. Scientia Horticulturae, 129(2), 176-182.
  14. Mneney, E. E. & Mantell, SH. (2001). In vitro micrografting of cashew. Plant cell, tissue and organ culture, 66(1), 49-58.
  15. Mojtahedi, N., Fathi Gharebaba, M. & Zohrabi, M. (2008). Developing a practical protocol for Mexican (Citrus aurantifolia) and Persian lime (Citrus latifolia). Final report of project. Ministry of Jahad-e-Agriculture Research and Education Organization, Agricultural Biotechnology Research Institute of Iran, 41p. (in Farsi)
  16. Mukhtar, R., Khan, M. M., Rafiq, R., Shahid, A. & Khan, F. (2005). In vitro regeneration and somatic embryogenesis in (Citrus aurantifolia and Citrus sinensis). International Journal of Agriculture and Biology, 7(3), 518-520.
  17. Navarro, L. (1987). Application of shoot-tip grafting in vitro to woody species. ISHS Acta Horticulturae, 227, 43-56.
  18. Onay, A., Pirinç, V., Yildirim, H. & Basaran, D. (2004). In vitro micrografting of mature pistachio (Pistacia vera var. Siirt). Plant Cell, Tissue and Organ Culture, 77(2), 215-219.
  19. Pathirana, R. & McKenzie, M. J. (2005).A modified green-grafting technique for large-scale virus indexing of grapevine (Vitis vinifera L.). Scientia horticulturae, 107(1), 97-102.
  20. Pliego-Alfaro, F. & Murashige, T. (1987). Possible rejuvenation of adult avocado by graftage onto juvenile rootstocks in vitro. HortScience, 22(6), 1321-1324.
  21. Prakash, O., Sood, A., Sharma, M. & Ahuja, P. (1999). Grafting micropropagated tea (Camellia sinensis L. Kuntze) shoots on tea seedlings-a new approach to tea propagation. Plant Cell Reports, 18(10), 883-888.
  22. Rafail, S. & Mosleh, M. (2010). Factors involved in micropropagation and shoot tip grafting of apple (Malus domestica Borkh.) and pear (Pyrus sp. L.). Tropentag, World Food System–A Contribution from Europe, September: 14-16.
  23. Raharjo, S. H. & Litz, R. E. (2005). Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana). Plant Cell, Tissue and Organ Culture, 82(1), 1-9.
  24. Singh, B., Sharma, S., Rani, G., Hallan, V., Zaidi, A., Virk, G. & Nagpal, A. (2008). In vitro micrografting for production of Indian citrus ringspot virus (ICRSV)-free plants of kinnow mandarin (Citrus nobilis Lour × C. deliciosa Tenora). Plant Biotechnology Reports, 2(2), 137-143.
  25. Suarez, I. E., Schnell, R. A., Kuhn, D. N. & Litz, R. E. (2005). Micrografting of ASBVd-infected Avocado (Persea americana) plants. Plant cell, tissue and organ culture, 80(2), 179-185.
  26. Tallón, C. I., Porras, I. & Pérez-Tornero, O. (2013). High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium. In Vitro Cellular and Developmental Biology-Plant, 49(2), 145-155.
  27. Tanne, E., Shlamovitz, N. & Spiegel-Roy, P. (1993). Rapidly diagnosing grapevine corky-bark by in vitro micrografting. HortScience, 28(6), 667-668.
  28. Wu, H., Du Toit, E. S. & Reinhardt, C. (2007). Micrografting of Protea cynaroides. Plant Cell, Tissue and Organ Culture, 89(1), 23-28.
Iranian Journal of Horticultural Science
Volume 49, Issue 2
August 2018
Pages 505-514

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History

  • Receive Date: 05 January 2017
  • Revise Date: 23 August 2017
  • Accept Date: 23 August 2017

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