Expression analysis of gaLFY and AsFT during reproductive development in different organs of some Iranian Garlic (Allium sativum L.) Clones

Document Type : Full Paper

Authors

1 Ph. D. Candidate, Faculty of Agriculture, Science, Bu-Ali Sina University, Hamedan, Iran

2 Associate Professor, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

3 Assistant Professor, Ramin Agriculture and Natureal Resource University of Khuzestan, Iran

Abstract

A true understanding of the temporal and spatial expression patterns of the genetic coding of florogenesis in fertile garlic genotypes will facilitate garlic breeding. Herein we studied the expression patterns of gaLFY and AsFT 1, 2, 4 during reproductive development indifferent organs ofbolting, semi-bolting and non-bolting Iranian garlic clones. The highest relative expression of the gaLFY was observed in the meristem of all clones eight weeks after planting, which was 20 fold more in bolting than in non-bolting clone. gaLFY expression was also increased in the inflorescence of bolting clone in comparison to control (eight leaves of non-bolting clone). AsFT4 and AsFT1 were expressed in the bulbs of all clones and at low amount in inflorescence and flowers of bolting clone, respectively. The highest relative expression of the AsFT2 was observed in the leaves of all clones 12 weeks after culture, which was 3.2 times more in bolting than in non-bolting clones. AsFT2 was also expressed in the meristem of bolting and semi-bolting clones. It is suggested that gaLFY plays its role in flowering integration and floral identity and AsFT2 could be a flowering integrator gene in bolting clone. Low expression of gaLFY and AsFT2 in semi- and non-bolting clones causes vegetative to reproductive transition but inhibits complete scape elongation. So that in the non-bolting garlic scape aborts and in the semi-bolting garlic a very short scape forms among the leaves.

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References

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History

  • Receive Date: 06 July 2017
  • Revise Date: 15 November 2017
  • Accept Date: 28 November 2017

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