Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Study of factors affecting hairy roots induction and Rosmarinic Acid production in Dracocephalum kotschyi Boiss

Document Type : Full Paper

Authors
Bahman Hosseini 1
Nasrin Ayyobi 2
Mohammad Fattahi 3
1 Associate Professor, Faculty of Agriculture, Urmia University, Iran
2 Former M.Sc. Student, Faculty of Agriculture, Urmia University, Iran
3 Assistant Professor, Faculty of Agriculture, Urmia University, Iran
10.22059/ijhs.2017.215380.1078
Abstract
Abstract: The flavonoid compounds in the Dracocephalum kotschyi has anti-cancer properties and using various methods such as hairy roots production can used to produce valuable secondary metabolites. In this study, the effect of explant type (cotyledon, hypocotyl, leaf and internode) and explants age (1 week leaf, 2 weeks leaf and 4 weeks old leaf) on hairy roots induction percent were surveyed. The transgenic status of hairy roots was confirmed by PCR using rolB genes-specific primers. In order to find optimum culture media for hairy roots growth and proliferation, four types of liquid basal media including, MS, 1/2MS, 1/4 MS and B5 and UV-B elicitor effects in three levels (3 min, 6 min and 9 min) on biochemical characteristics and Rosmarinic acid (RA) contents were investigated. The maximum hairy roots induction was obtained in 1 week leaves old (56.66 %); cotyledon explants (36.66 %), respectively. The highest fresh (4.34 gr) and dry weight (0.31 gr) and lowest fresh (1.04 gr) and dry weight (0.10 gr) was recorded in 1/4 MS and B5 media, respectively. The maximum and minimum antioxidant activities (92.33% and 63 %) were obtained in UV-B exposure time to 6 min radiation and non-transformed hairy roots. High performance liquid chromatography analysis revealed that the production of RA in UV-B (6 min) elicited hairy roots of D. kotschyi was almost 2.5-fold higher than that of the non-transformed roots (34.9 µg /mg DW compared to 13.8 µg /mg DW).
Keywords
Agrobacterium rhizogenes
antioxidant activitiy
Catalase
Total Flavonoid
Zarrin-giah
Subjects
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Iranian Journal of Horticultural Science
Volume 49, Issue 1 - Serial Number 1
Spring 2018
Pages 253-267

  • Receive Date 05 October 2016
  • Revise Date 15 March 2017
  • Accept Date 16 May 2017