Regeneration, proliferation and commercialized micro propagation of Anthurium andraeanum cv. Pink

Document Type : Full Paper

Authors

1 Assist Professor, Faculty of Agriculture, University of Zanjan, Iran

2 Former M.Sc. Student, Faculty of Agriculture, University of Zanjan, Iran

3 Assistant Professor, Institute of Biological Technologies, University of Zanjan, Iran

4 Assistant Professor, Faculty of Agriculture, University of Zanjan, Iran

Abstract

A standard in vitro mass propagation protocol for development and propagation of valuable flowers such as Anthurium is one of the major procedures and goals in flower industry. Towards developing a commercialized propagation recipe for A. andraeanum cv. Pink, effects of BA and NAA were investigated on regeneration, proliferation and in vitro mass propagation efficiency of cultivar Pink, as well as quantitative and qualitative characteristics. The lateral buds were established in 1/2MS contains different concentrations of BA (0, 0.1, 0.3 mg/l) and NAA (0, 0.04, 0.07, 0.23 mg/l). The longest shoots (average of 23.46 mm), was obtained in 1/2MS containing 0.23 mg/l NAA .The highest number of buds were produced in medium containing 0.23 NAA plus 0.3mg/l BA. The highest root number was emerged in 0.23 mg/l NAA. The highest percentage of regeneration rate obtained in the media containing 0.23mg/l NAA+ 0.3mg/l BA compared to the control. Obtained protocol can be simply used for in vitro mass propagation of healthy Anthurium plants.  

Keywords

Main Subjects

References

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History

  • Receive Date: 05 February 2017
  • Revise Date: 01 June 2017
  • Accept Date: 09 July 2017

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