Effect of irrigation interval and silicon on some morpho-physiological and biochemical properties of basil (Ocimum basilicum L.)

Document Type : Full Paper


1 Former M.Sc. Student, Department of ACECR Institute of Higher Education in Kermanshah, Iran

2 Associate Professor, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

3 Associate Professor, Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran


In order to investigate the effect of irrigation interval and silicon on growth, yield and oil content, total phenols and antioxidant activity of basil, an experiment was carried out at research greenhouse of Faculty of Agriculture, Lorestan University, Iran, in 2016. The research was carried out in pots as a factorial experiment based on a completely randomized design with four replications. Factors consisted of irrigation in three intervals of 4, 7 and 9 days (according to soil water content of 90%, 75% and 60% field capacity, respectively) and weekly foliar application of 0, 1 and 2 mM potassium silicate. Results showed that increasing irrigation interval decreased growth, and increased water use efficiency. Silicon alleviated the stress effects and improved growth parameters and application of 2 mM potassium silicate increased stress tolerance index. Both higher irrigation intervals and foliar application of silicon increased oil content and total phenols.Moreover, increasing irrigation intervals increased antioxidant activity and the maximum activity was found in plants irrigated every 9 d and foliar sprayed with 2 mM potassium silicate. Correlation coefficient indices showed that oil content had significant positive correlations with root length, total phenols and stress tolerance index and a negative correlation with IC50. Overall, results showed that increase in irrigation interval induced drought stress and consequently decreased the growth parameters measured in this experiment and application of potassium silicate alleviated the effects of drought and improved plant tolerance to drought stress and it increased oil content, oil yield and antioxidant activity.


Main Subjects

  1. Ainsworth, E. A. & Gillespie, K. M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature protocols, 2(4), 875-877.
  2. Anonymous. (1986). Hungarian Pharmacopoeia. Volume III. Medicina Konyvkiado. Budapest. Hungary.
  3. Aslani, Z., Hassani, A., Rasooli Sadaghiyani, M., Sefidkon, F. & Barin, M. (2011). Effect of two fungi species of arbuscular mycorrhizal (Glomus mosseae and Glomus intraradices) on growth, chlorophyll contents and P concentration in Basil (Ocimum basilicum L.) under drought stress conditions.Iranian Journal of Medicinal and Aromatic Plants, 27(3), 471-486. (in Farsi)
  4. Baher, Z. F., Mirza, M., Ghorbanli, M. & Bagher Rezaii, M. (2002). The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal, 17(4), 275-277.
  5. Chen, W., Yao, X., Cai, K. & Chen, J. (2011). Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biological trace element research, 142(1), 67-76.
  6. Eraslan, F., Inal, A., Pilbeam, D. J. & Gunes, A. (2008). Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinacia oleracea L. cv. Matador) grown under boron toxicity and salinity. Plant Growth Regulation, 55(3), 207-219.
  7. Gharibi, S., Sayed Tabatabaei, B. E., Saeidi, G., Goli, S. A. H. & Talebi, M. (2012). Effect of drought stress on some physiological properties and antioxidant activity of Achillea tenuifolia Lam. Journal of Herbal Drugs, 3(3), 181-190. (in Farsi)
  8. Goldani, M. (2012). Effect of irrigation interval on some growth characteristics of basil ecotypes. Iranian Journal of Field Crops Research, 10(2), 412-420. (in Farsi)
  9. Hamzezadeh, M., Fathi, P., Javadi, T. & Hassani, A. (2011). The effect of different irrigation water levels on water use efficiency in basil plant (Ocimum Basilicum var. Keshkeny Levelu) using marginal analysis theory. Journal of Water and Soil, 25(5), 953-960. (in Farsi)
  10. Hassani, A. & Omidbaigi, R. (2002). Effects of water stress on some morphological, physiological and metabolic properties of basil. Iranian Journal of Agriculture Science, 12(3), 44-61. (in Farsi)
  11. Hassani, A., Omidbaigi, R. & Heidari Sharif Abad, H. (2004). Study of some drought resistance indices in basil (Ocimum basilicum L.). Journal of Agricultural Sciences and Natural Resources, 10(4), 64-75. (in Farsi)
  12. Hejazi Mehrizi, M., Shariatmadari, H., Khoshgoftarmanesh, A. H. & Moattar, F. (2011). Effects of salinity and zinc nutrition on growth and antioxidant properties of Rosmarinus officinalis L. in a calcareous soil. Iranian Journal of Medicinal and Aromatic Plants, 27(1), 25-35. (in Farsi)
  13. Hornok, L. (1992). Cultivation and processing of medicinal plants. Academic publication. Budapest, Hungary. 338 pp.
  14. Javanmardi, J., Khalighi, A., Kashi, A., Bais, H. P. & Vivanco, J. M. (2002). Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. Journal of agricultural and food chemistry, 50(21), 5878-5883.
  15. Kang, S., Shi, W. & Zhang, J. (2000). An improved water-use efficiency for maize grown under regulated deficit irrigation. Field crops research, 67(3), 207-214.
  16. Kaya, C., Tuna, L. & Higgs, D. (2006). Effect of silicon on plant growth and mineral nutrition of maize grown under water-stress conditions. Journal of Plant Nutrition, 29(8), 1469-1480.
  17. Lei, Y., Korpelainen, H. & Li, C. (2007). Physiological and biochemical responses to high Mn concentrations in two contrasting Populus cathayana populations. Chemosphere, 68(4), 686-694.
  18. Liang, Y., Sun, W., Zhu, Y. G. & Christie, P. (2007). Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environmental pollution, 147(2), 422-428.
  19. Lutts, S., Kinet, J. M. & Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78(3), 389-398.
  20. Miliauskas, G., Venskutonis, P. R. & Van Beek, T. A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry, 85(2), 231-237.
  21. Moghaddam, M., Alirezaei Noghondar, M., Selahvarzi, Y. & Goldani, M. (2015). The effect of drought stress on some morphological and physicochemical characteristics of three cultivars of basil (Ocimum basilicum L.). Iranian Journal of Horticultural Science, 46(3), 509-521. (in Farsi)
  22. Morgan, J. M. (1984). Osmoregulation and water stress in higher plants. Annual review of plant physiology, 35(1), 299-319.
  23. Omidbaigi, R. (2000). Production and processing of medicinal plants. Volume 3. Astan-e-Ghods-e-Razavi, Mashhad, Iran. 397 pp.
  24. Rassam, G., Dadkhah, E. and Khoshnood Yazdi, A. (2015). Evaluation of water deficit on morphological and physiological traits of Hyssop (Hyssopus officinalis L.). Journal of Agronomy Sciences, 5(10), 1-12. (in Farsi)
  25. Ritchie, S. W., Nguyen, H. T. & Holaday, A. S. (1990). Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop science, 30(1), 105-111.
  26. Simon, J. E., Reiss-Bubenheim, D., Joly, R. J. & Charles, D. J. (1992). Water stress-induced alterations in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4(1), 71-75.
  27. Taiz, L. & Zeiger, E. (2010). Plant Physiology. 5th Ed. Sunderland: Sinauer Assoc. USA.782 pp.