Evaluation of tolerance and remediation of ornamental kale to heavy metals in the process of development

Document Type : Full Paper


1 Assistant Professor, Faculty of Agriculture & Natural Resources, Arak University, Arak, Iran

2 Former M. Sc. Student, Faculty of Agriculture & Natural Resources, Arak University, Arak, Iran


Todays, the accumulation and toxicity of heavy metals in the environment and food chain is one of the most major problems in the world. Recently, remediation of these metals have been considered by the plants. This study was performed in two experiments. Lead, zinc and copper concentrations 0, 20, 50, 100 and 200 mg/l was applied during seed germination of ornamental cabbage, in the first experiment. In the second experiment, tolerance and remediation of ornamental cabbage to lead, zinc and copper concentrations 0, 25, 50 mg/l were investigated at the greenhouse conditions. In the first experiment with increasing concentrations of heavy metals, growth of seedlings was decreased. The highest seedling growth inhibitory effect was observed in the presence of copper, lead and zinc, respectively. In greenhouse experiments, the highest growth and lowest toxicity were observed in the presence of Cu, Zn and Pb, respectively in the media contained vermicompost. Amount of Cu and Pb uptake was found more in the bed without vermicompost. The achieved results indicated that ornamental cabbage could be as new plant hyperaccumulator and has potential for use in the Phytoremediation study of Pb and Cu contaminated soils.


Main Subjects

  1. Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in beta vulgaris. Plant Physiology, 24, 1-15.
  2. Bajji, M., Kinet, J. M. & Lutts, S. (2002). Osmotic and ionic effects of NaCl on germination, early seeding growth, ion content of Atriplex halimus (Chenopodiaceae). Canadian Journal of Botany, 80, 297-304.
  3. Beiki, A. H. & Allahyari, S. (2011). Effects of organic amendments on reduction the heavy metals concentration of absorbable in soil lime of Zanjan. In: Proceedings of 1st National Conference Modern Topicin Agriculture, Oct., Azad University, Saveh. (in Farsi)
  4. Carrasquero-Duran, A. & Flores, L. (2009). Evaluation of lead (II) immobilization by a vermicompost using adsorption isotherms and IR spectroscopy. Bioresource Technol, 100, 1691-1694.
  5. Chand, S., Raj, K. & Patra, D. D. (2012). Influence of nickel and applied in combination with vermicompost growth and accumulation of heavy metals by Mentha arvensis Linn. Cv. Kosi.Indian Journal of Products and Resources, 3(2), 256-261.
  6. Chockalingam, E., & Subramanian, S. (2006). Studies on removal of metal ions and sulphate reduction using rice husk and Desulfotomaculum nigrificans with reference to remediation of acid mine drainage. Chemosphere, 62(5), 699-708. 
  7. Dolgen, D., Alpaslan, M. N. & Delen, N. (2007). Agricultural recycling of treatment-plant sludge: A case study for a vegetable-processing factory. Journal of Environmental Management, 84, 274-281.
  8. Dushenkov, V., Kumar, P. B. A. N., Motto, H. & Raskin, I. (1995). Rhizofiltration: the use of plants to remove heavy metals from aqueous streams. Environmental Science and Technology, 29, 1239-1245.
  9. Fargasova, A. (2004). Toxicity comparison of some possible toxic metals (Cd, Cu, Pb, Se, Zn) on young seedlings of Sinapis alba L. Plant Soil Environ. 50, 33-38.
  10. Fodor, F., Prasad, M. N. V. & Strzalka, K. (2002). Physiological responses of vascular plants to heavy metals. In Physiology and biochemistry of metal toxicity and tolerance in plants. Kluwer Academic Publisher, Dortrech, London. pp. 149-177.
  11. Ghosh, M. & Singh. S. P. A. (2005). Review on Phytoremediation of Heavy Metals and Utilization of It’s by Products. Journal Energy Environmental, 6(04), 214-231.
  12. Hall, J. L. & Williams, L. E. (2003). Transition metal transporters in plants. Journal of Experimental Botany, 54, 2601-2613.
  13. Hashemimajd, K. & Golchin, A. (2009). The Effect of Iron-Enriched Vermicompost on Growth and Nutrition of Tomato. Journal of Agriculture Science and Technology, 11, 613-621. (in Farsi)
  14. Jaja, E. T. & Odoemena, C. S. I. (2004). Effect of Pb, Cu and Fe compounds on the germination and early seedling growth of tomato varieties. Journal of Applied Sciences and Environmental Management, 8(2), 51- 53.
  15. Jurries, D. P. E. (2004). Environmental protection and enhancement with compost, Department of Environmental Quality Northwest Region, Oregon State University, USA.
  16. Kabir, L., Iqbal, M., Shafiq, M. Z. & Farooqi, Z. R. (2010). Effects of lead on seedling growth of Thespesia populnea. Plant Soil Environmental, 56(4), 194-199.
  17. Keshtegar, M., Akbari M. A., Rostami, R. & Jahantigh, M. (2013). Investigation of plants purification capability of Pb on two cultivars of vetch plants (Vigna radiata) in contaminated soils. International Research Journal of Applied and Basic Sciences, 7(13), 983-987. 
  18. Lidon, F.C. & Henriques, F. S. (1998). Role of rice shoot vacuoles in copper toxicity regulation. Environmental of Experimental Botany, 39, 197-202.
  19. Lone, M. I., Li, H., Zhen, P. J., Stoffella, E. & Yang, X. (2008). Phytoremediation of heavy metal polluted soils and water: Progresses and perspectives. Journal of Zhejiang University Science Botany, 9, 210-220.
  20. Mishra, A. & Choudhuri, M. A. (1998). Amelioration of lead and mercury effects on germination and rice seedling growth by antioxidants. Biologia Plantarum, 41(3), 469-473.
  21. Molaei, S., Shirani, H., Hamidpour, M., Shekofteh, H. & Besalatpour, A. A. (2016). Effect of Vermicompost, Pistachio Kernel and Shrimp Shell on Some Growth Parameters and Availability of Cd, Pb and Zn in Corn in a Polluted Soil. JWSS - Isfahan University of Technology, 19(74), 113-124.
  22. Mourato, M. P., Inês, N., Moreira, Inês Leitão, Filipa R. Pinto, Joana R. Sales. & Martins L. L. (2015). Effect of Heavy Metals in Plants of the Genus Brassica .International Journal Molecular Science, 16, 17975-17998.
  23. Muscolo, A., Bovalo, F., Gionfriddo, F. & Nardi, F. (1999). Earthworm humic matter produces auxin-like effects on Daucus carota cell growth and nitrate metabolism. Soil Biology and Biochemistry, 31, 1303-1311.
  24. Nriagu, J. O. & Pacyna, J. M. (1988). Quantitative assessment of worldwide contamination of air, water and soil by trace metals. Nature, 333, 134-139.
  25. Ozkay, F., Kiran, S., Taş, İ. & Kuşvuran, Ş. (2014). Effects of Copper, Zinc, Lead and Cadmium Applied with Irrigation Water on Some Eggplant Plant Growth Parameters and Soil Properties. Türk Tarım ve Doğa Bilimleri Dergisi, 1(3), 377-383.
  26. Peralta, J. R., Gardea, Torresdey, J. L., Tiemann, K. J., Gómez, E., Arteaga, S., Rascon, E. & Parasons, J. G. (2000). Study of the heavy metals on seed germination and plant growth on alfalfa plant (Medicago sativa) Growth in solid media. In Proceedings of the Conference on Hazardous Waste Research, 135-140.
  27. Prasad, M. N. V. & Freitas, H. M. O. (2003). Metal hyperaccumilation in plants-Biodiversity prospecting for phytoremediation technology. Electronic Journal of Biotechnology, 6, 285-321.
  28. Purohit, S.S. & Agrrawal, A. K. (2006). Environmental Pollution. Agrobios Publication. India.
  29. Robinson, B. H., Banuelos, G., Conesa, H. M., Evangelon, W. H. & Schulin, R. (2009). The Phytomanagement of Trace Elements in Soil. Critical Reviews in Plant Sciences, 28(4), 240-266.
  30. Rostami Zadeh, A., Mohsen pour, M. & Fekri M. (2013). The effect of vermicompost on the absorption of iron and zinc in maize. In proceeding 1th National Conference on e-agriculture and sustainable natural resources. Tehran, Jan. (in Farsi)
  31. Seelsaen, N., McLaughlan, R., Moore, S., & Stuetz, R. (2006). Influence of compost characteristics on heavy metals sorption from synthetic storm water. Journal Urban Drainage Modelling and Water Sensitive Urban Design, 55(4), 219-226.
  32. Sekabira, K., Oryem-Origa, H., Mutumba, G., Kakudidi, E. & Basamba, T. A. (2011). Heavy metal phytoremediation by Commelina benghalensis (L) and Cynodon dactylon (L) growing in Urban stream sediments. International Journal of Plant Physiology and Biochemistry, 3(8), 133-142.
  33. Sharifi, M., Afyuni, M. & Khoshgoftarmanesh, A. H. (2010). Effect of Sewage Sludge, Compost and Cow Manure on Growth and Yield and Fe, Zn, Mn and Ni Uptake in Tagetes Flower. Journal of Science and Technology of Greenhouse Culture, 1(2), 43-54. (in Farsi)
  34. Sunitha, R., Mahimairaja, S., Bharani & Gayathri, A. P. (2014). Enhanced Phytoremediation Technology for Chromium contaminated Soils using Biological Amendments.  International Journal of Science and Technology, 3, 3.
  35. Taghizadeh, M., Kafi, M., Fattahi Moghaddam, M. R. & Savaghebi, G. H. (2002). Lead concentrations effect on seed germination of turfgrass genius and its evaluation potential for phytoremediation. Iranian Journal of Horticultural Science, 42(3), 277-289. (in Farsi)
  36. Tu, C., Ma, L. Q. & Bondada, B. (2002). Arsenic Accumulation in the Hyperaccumulator Chinese Brake and Its Utilization Potential for Phytoremediation. Journal Environmental Quality, 31, 1671-1675.
  37. Wierzbicka, M. & Obidzinska, J. (1998). The effect of lead on seed imbibition and germination in different plant species. Plant Science, 137, 155-171.
  38. Yruela, I. (2005). Copper in plants. brazilian Journal plant physiology, 17(1), 145-156.
  39. Zheljazkov, V. D. & Warman, P. R. (2004). Application of high- Cu compost to Dill and Peppermint, Journal Agriculture and Food Chemistry, 52, 2615-2622. (in Chinese with English abstract)