Effect of edible coatings on maintenance of bioactive compounds and increasing the storability of pomegranate aril cv. Rabbab-e-Neyriz

Document Type : Full Paper


1 Associate Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran

2 Former M.Sc. Student, Faculty of Agriculture, Shiraz University, Shiraz, Iran

3 Ph.D. Candidate, Faculty of Agriculture, Shiraz University, Shiraz, Iran


In this research, the effect of chitosan, nano-chitosan, methylcellulose, and pectin on maintenance of bioactive compounds and storability of pomegranate aril cv. Rabbab-e-Neyriz were studied during storage time. Arils were treated with 1.5% chitosan, 3% methylcellulose, 3% pectin and pure nano-chitosan (100%) solutions and then stored at 5 °C for 16 days and sampling of stored arils was done at 4 days intervals. After 16 days of storage at 5 °C, fungal contamination was observed in all samples except for those treated with 1.5% chitosan. The most and the least weight loss in arils obtained in the control and those treated with 1.5% chitosan, respectively. The edible coatings especially chitosan significantly prevented from degradation of anthocyanin and ascorbic acid. Total phenoland antioxidant activity of juice decreased during storage; however chitosan coating significantly led tomaintain these bioactive compounds. Edible coating treatments and especially chitosan maintained the TSS, TA, flavor index in comparison with control. Overall, chitosan effectively reduced water loss, aril losses and maintained bioactive compounds during storage.


Main Subjects

  1. Al-Maiman, S. A. & Ahmad, D. (2002). Changes in physical and chemical properties during pomegranate (Punica granatum L.) fruit maturation. Food Chemistry, 76, 437-441.
  2. Aydin, N. & Kadioglu, A. (2001). Changes in the chemical composition, polyphenol oxidase and peroxidase activities during development and ripening of Medlar fruits (Mespilus germanica L.). Bulgarian Journal of Plant Physiology, 27, 85-92.
  3. Ayranci, E. & Tune, S. (2004). The effect of edible coatings on water and vitamin C loss of apricots (Armeniaca vulgaris Lam.) and green peppers (Capsicum annuum L.). Food Chemistry, 87, 339-342.
  4. Bico, S. L. S., Raposo, M. F. J., Morais, R. M. S. C. & Morais, A. M. M. B. (2009). Combined effects of chemical dip and/or carrageenan coating and/or controlled atmosphere on quality of fresh cut banana. Food Control, 20, 508-514.
  5. Çam, M., Hışıl, Y. & Durmaz, G. (2009). Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food Chemistry, 112, 721-726.
  6. D’Aquino, S., Palma, A., Schirra, M., Continella, A., Tribulato, E. & La Malfa, S. (2010). Influence of film wrapping and fludioxonil application on quality of pomegranate fruit. Postharvest Biology and Technology, 55, 121-128.
  7. Dhall, R. K. (2013). Advances in edible coatings for fresh fruits and vegetables: a review. Critical Reviews in Food Science and Nutrition, 53, 435-450.
  8. Ghasemnezhad, M., Zareh, S., Rassa, M. & Sajedi, R. H. (2013). Effect of chitosan coating on maintenance of aril quality, microbial population and PPO activity of pomegranate (Punica granatum L. cv. Tarom) at cold storage temperature. Journal of the Science of Food and Agriculture, 93, 368-374.
  9. Ghasemnezhad, M., Zareh, S., Shiri, M. A. & Javdani, Z. (2015). The arils characterization of five different pomegranate (Punica granatum) genotypes stored after minimal processing technology. Journal of Food Science and Technology, 52, 2023-2032.
  10. Hana, A., Zhao, S., Leonard, W. & Traber, M. G. (2004). Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria ananasa) and raspberries (Rubus ideaus). Postharvest Biology and Technology, 33, 67-78.
  11. Klein, B. & Perry, A. (2006). Ascorbic acid and vitamin A activity in selected vegetables from different geographical areas of the United States. Journal of Food Science, 47, 941-945.
  12. Li, H. & Yu, T. (2000). Effect of chitosan on incidence of brown rot, quality and physiological attributes of postharvest peach fruit. Journal of the Science of Food and Agriculture, 81, 269-274.
  13. Meyers, K. J., Watkins, C. B., Pritts, M. P. & Liu, R. H. (2003). Antioxidant and anti-proliferative activities of strawberries. Journal of Agricultural and Food Chemistry, 51, 6887-6892.
  14. Miguel, G., Fontes, C., Antunes, D., Neves, A. & Martins, D. (2004). Anthocyanin concentration of ‘Assaria’ pomegranate fruits during different cold storage conditions. Journal of Biomedicine and Biotechnology, 5, 338-342.
  15. Ozgen, M., Dugac, C., Serce, S. & Kaya, C. (2008). Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey. Food Chemistry, 111, 703-706.
  16. Pierce, B. & Kader, A. (2003). Responses of ‘Wonderful’ pomegranates to controlled atmospheres. Acta Horticulturae, 600, 751-757.
  17. Ramezanian, A., Rahemi, M. & Vazifehshenas, M. R. (2009). Effects of foliar application of calcium chloride and urea on quantitative and qualitative characteristics of pomegranate fruits. Scientia Horticulturae, 121, 171-175.
  18. Ramezanian, A., Rahemi, M., Maftoun, M., Bahman, K., Eshghi, S., Safizadeh, M. R. & Tavallali, V. (2010). The ameliorative effects of spermidine and calcium chloride on chilling injury in pomegranate fruits after long-term storage. Fruits, 65, 169-178.
  19. Rapisarda, P., Fanella, F. & Maccarone, F. (2000). Reliability of analytical methods for determining anthocyanin in blood orange juices. Journal of Agricultural and Food Chemistry, 48, 2249-2252.
  20. Sayyari, M., Babalar, M., Kalantari, S., Martinez-Romero, D., Guillen, F., Serrano, M. & Valero, D. (2011). Vapor treatments with methyl salicylate or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates. Food Chemistry, 124, 964-970.
  21. Shahidi, F., Arachchi, J. K. V. & Jeon, Y. J. (1999). Food applications of chitin and chitosan. Journal of Food Science and Technology, 10, 37-51.
  22. Tulinoz, A. & Ulukanli, Z. (2011). Application of edible starch-based coating including glycerol plus oleum nigella on arils from long-stored whole pomegranate fruits. Journal of Food Processing and Preservation, 36, 81-95.
  23. Varasteh, F., Arzani, K., Barzegar, M. & Zamani, Z. (2012). Changes in anthocyanin in arils of chitosan-coated pomegranate (Punica granatum L. cv. Rabbab-e-Neyriz) fruit during cold storage. Food Chemistry, 130, 267-272.
  24. Vargas, M., Albors, A., Chiralt, A. & González-Martínez, C. (2006). Quality of cold-stored strawberries as affected by chitosan–oleic acid edible coatings. Postharvest Biology and Technology, 41, 164-171.
  25. Yaman, O. & Bayindirli, L. (2002). Effects of an edible coating and cold storage on shelf-life and quality of cherries. Lebensmittel-Wissenschaft and Technologie, 35, 146-150.
  26. Yen, M. T., Yang, J. H. & Mau. J. L. (2009). Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymers, 75, 15-21.