Effects of plant growth regulators and ultrasound treatments on in vitro rooting and callogenesis of Lilium ledebourii Boiss.

Document Type : Full Paper


1 Former M. Sc. Student, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran

2 Assistant Professor, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran

3 Assocaite Professor, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran


This study was performed to evaluate the effect of NAA, BA and ultrasound waves on in vitro rooting and callus induction of Lilium ledebourii. For this purpose, scale explants after exposure to an ultrasonic bath with a frequency of 35 kHz in times 0, 5, 10, 20 and 30 seconds were cultured on MS medium containing different concentrations (0, 0.01, 0.1 and 1 mg l-1) of NAA and BA alone or in combination with each other. This study was carried out in four experiments including combination of NAA and BA, NAA and ultrasound, BA and ultrasound, and the best treatments which achieved from first experiment with ultrasound, respectively. In the first and second experiment, 1 mg l-1 NAA treatments had the highest root induction and in the third experiment control treatment had the better result. In the second experiment, ultrasound did not stimulate root induction, however, in the third experiment, in 0 and 1 mg l-1 BA concentrations, ultrasound had no positive effect on root induction, but at low concentrations, BA increased the number of roots. Moreover, it was indicated that plant growth regulators and ultrasound had no significant effect on callus induction. In this study, concentration of 1 mg l-1 NAA was the best treatment for increasing of rooting parameters in scale explants.


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