ACO gene expression and catalase activity under the influence of salicylic acid and plant extracts in two miniatures Carnation cut flowers

Document Type : Full Paper


1 Former M. Sc. Student, Sari Agricultural Sciences & Natural Resources University, Sari, Iran

2 Assistant Professor, Sari Agricultural Sciences & Natural Resources University, Sari, Iran


Carnation is one of the most important cut flowers in the world. The flower is to reduce the economic value due to its short vase life. Some materials can be used to help increase the vase life of cut flowers. According to this, using salicylic acid hormone and plant extracts treatment increased the shelf life of cut flowers. Factorial experiment based on a completely randomized design was conducted with 10 treatments (Different concentrations of salicylic acid, Savory and hemp extracts) and four replications with two varieties of miniature carnation. Factors such as vase life, antioxidant enzymes catalase and the relative gene expression ACC oxidase (ACO) were evaluated. Results showed that the interaction of treatments and cultivars was effective in vase life. Spectro cultivar vase life was more than a pretty tessino cultivar. Salicylic acid at 150ppm increased vase life and catalase activity. In addition, this treatment decreased relative ACO gene expression more than the other treatments and control. Savory and hemp extract treatments at high concentrations increased vase life as well as catalase activity, but ACO expression decreased. So it seems that the use of these treatments can be complementary or good alternative to other chemical treatments.


  1. Abraham, H., Halevy, H. & Shimon, M. (1982). Senescence and postharvest physiology of cut flowers. Horticultural Reviews, 10, 8-123.
  2. Agricultural statistics. (2008). Department of Agriculture. (in Farsi)
  3. Ávila, F. W., Faquin, V., da Silva Lobato, A. K., Ávila, P. A., Marques, D. J., Silva Guedes, E. M., & Tan, D. K. Y. (2013). Effect of phosphite supply in nutrient solution on yield, phosphorus nutrition and enzymatic behavior in common bean (Phaseolus vulgaris L.) plants. Australian Journal of Crop Science, 7(5), 713.
  4. Bayat, H., Azizi, M., Shuor, M. & Vahdati, N. (2011). The effect of ethanol and Essential Oil to increased vase life of carnation cut flowers (Dianthus caryophyllus cv. Yellow Candy). Journal of Horticultural Science, 25(4), 384-390. (in Farsi)
  5. Bounatirou, S., Smiti, S., Miguel, M. G., Faleiro, L., Rejeb, M. N., Neffati, M., ... & Pedro, L. G. (2007). Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. et Link. Food Chemistry, 105(1), 146-155.
  6. Cakmak, I. & Horst, W. J. (1991). Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 83(3), 463-468.
  7. Da Silva, J. A. T. (2003). The cut flower: postharvest considerations. Biological Sciences, 110, 24-27.
  8. Damunupola, J. W., Qian, T., Muusers, R., Joyce, D. C., Irving, D. E. & Van Meeteren, U. (2010). Effect of S-carvone on vase life parameters of selected cut flower and foliage species. Postharvest biology and technology, 55(1), 66-69.
  9. Doel, J. M. & Wilkins, H. F. (1999). Floriculture: principles and species. Prentic-Hall, Inc. New Jersy. 613p.
  10. Du, Y. Y., Wang, P. C., Chen, J. & Song, C. P. (2008). Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana. Journal of Integrative Plant Biology, 50(10), 1318-1326.
  11. Galbally, J. & Galbally, E. (1997). Carnations and Pinks for garden and greenhouse. Timber Press, Portland Oregon, USA. 310P.
  12. Halevy, A. H. & Mayak, S. (1997). Senescence and postharvest physiology of cut flower. Part 1. Horticulture Review, 1, 204-236.
  13. Hashemi, M., Mirdehghan, S. H., Farahmand, H. & Dashti, H. (2012). The effect of salicylic acid and methyl jasmonate on quality of vase life of cut flowers gerbera. Journal of Horticultural Science, 26(3), 311-320. (in Farsi)
  14. Hatamzadeh, A., Hatami, M. & Ghasemnezhad, M. (2012). Efficiency of salicylic acid delay petal senescence and extended quality of cut spikes of Gladiolus grandiflora cv ‘wing’s sensation’. African Journal of Agricultural Research, 7(4), 540-545.
  15. In, B. C., Binder, B. M., Falbel, T. G. & Patterson, S. E. (2013). Analysis of gene expression during the transition to climacteric phase in carnation flowers (Dianthus caryophyllus L.). Journal of Experimental Botany, 64(16), 4923-4937.
  16. Kazemi, M. & Shokri, K. (2011). Role of salicylic acid in decreases of membrane senescence in cut lisianthus flowers. Journal of World Applied Sciences, 13, 142-146.
  17. Kim, B. Y., Kim, H. J., Lee, K. S., Seo, S. J. & Jin, B. R. (2008). Catalase from the white-spotted flower chafer, Protaetia brevitarsis: cDNA sequence, expression, and functional characterization. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 149(1), 183-190.
  18. Lambert, R. J. W., Skandamis, P. N., Coote, P. J. & Nychas G. J. E. (2001). A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology, 91, 453-462.
  19. Mihajilov-krstev T., Radnovic D., Kitic D., Stojanovic-Radic Z. & Zlatkovic B. (2010). Antimicrobial activity of Satureja hortensis L. essential oil against pathogenic microbial strains. Archives of Biological Sciences, 62, 159-166.
  20. Moller, I. M. & Sweetlove, L. J. (2010). ROS signalling–specificity is required. Trends in Plant Science, 15(7), 370-374.
  21. Park, K. Y., Drory, A. & Woodson, W. R. (1992). Molecular cloning of an 1-aminocyclopropane-1-carboxylate synthase from senescing carnation flower petals. Plant Molecular Biology, 18(2), 377-386.
  22. Raskin, I. (1992). Role of salicylic acid in plants. Annual Review of Plant Biology, 43(1), 439-463
  23. Solgi, M., Kafi, M., Taghavi, T. S. & Naderi, R. (2009). Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesonii cv.‘Dune’) flowers. Postharvest Biology and Technology, 53(3), 155-158.
  24. Sood, S., Vyas, D. & Nagar, P. K. (2006). Physiological and biochemical studies during flower development in two rose species. Scientia Horticulturae, 108(4), 390-396.
  25. Sylvestre, I., Droillard, M. J., Bureau, J. M. & Paulin, A. (1989). Effects of the ethylene rise on the peroxidation of membrane lipids during the senescence of cut carnations. Plant Physiology and Biochemistry, 27, 407-13.
  26. Van Doorn, W. G. (1998). Effects of daffodil flowers on the water relations and vase life of roses and tulips. Journal of the American Society for Horticultural Science, 123(1), 146-149.
  27. van Doorn, W. G. & de Witte, Y. (1991). Effect of dry storage on bacterial counts in stems of cut rose flowers. HortScience, 26(12), 1521-1522.
  28. van Doorn, W. G., Zagory, D., de Witte, Y. & Harkema, H. (1991). Effects of vase-water bacteria on the senescence of cut carnation flowers. Postharvest Biology and Technology, 1(2), 161-168.
  29. Woltering, E. J. & Van Doorn, W. G. (1988). Role of ethylene in senescence of petals-morphological and taxonomical relationships. Journal of Experimental Botany, 39(11), 1605-1616.
  30. Yang, T. & Poovaiah, B. W. (2002). Hydrogen peroxide homeostasis: activation of plant catalase by calcium/calmodulin. Proceedings of the National Academy of Sciences, 99(6), 4097-4102.