Chilling tolerance improving of tomato seedling by drought stress pretreatment

Document Type : Full Paper


1 Former Ph.D. Student, Department of Horticultural Science, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran

2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran


Low temperatures lead to numerous physiological disturbances in the cells of chilling-sensitive plants, resulted in chilling injuries and death of tropical and subtropical plants such as tomatoes. In this study, the possibility of cold stress tolerance enhancement of tomato seedlings byimposing drought stress with 10 or 20 percent of polyethylene glycol (PEG) for 10 days was investigated in the greenhouse of agricultural faculty of Bu-Ali Sina University, Hamedan, Iran. The layout was factorial experiment in CRD design with four replications. After drought pretreatment, the seedlings were subjected to chilling 6 h/day at 3°C for 6 days. Drought pretreatment improved growth rate of tomato seedlings subjected to chilling stress as well as RWC, phenol, chlorophyll content and chlorophyll fluorescence ratio (Fv/Fm) when compared with the controls un-chilled seedlings. Interaction effects showed that highest value of root (2.25 g) and shoot fresh (4.4 g) weight, RWC (88.31 %), Fv/Fm(0.834) and total chlorophyll (1.62 mg/g F.W) were obtained in 0 % PEG under the chilling control. In contrast, the highest amounts of MDA (1.46 µm/g F.W) and phenol (10.86 mg/g F.W) content were obtained from 20% PEG and non-chilling stress. In general, results indicated that drought pretreatment could be used effectively to protect tomato seedlings from damaging effects of low temperatures stress at the early stages of growth.


  1. HassanPour, J. M., Kafi, M. & Mirhadi, M. J. (2008). Effect of drought stress on yield and some physiological characters in barley. Iranian Journal of Agricultural Science, 39, 165-177. (in Farsi)
  2. Javanmardi, J. (2009). Scientific and applied basis for vegetable transplant production. Jahade Daneshgahi Press, 256 pp. (in Farsi)
  3. Amundsun, R. G., Kohut, R. J., Laurence, J. A., Fellows, S., & Colavito, L. J. (1993). Moderate water stress alters carbohydrate content and cold tolerance of ted spruce foliage. Environmental and Experimental Botany, 33, 383-390.
  4. Food and Agriculture Organization. (2016). Retrieved April 10, 2016, from FAO website.
  5. Baldi, P., Pedron, L., Hietala, A. M. & Porta, N. A. (2011). Cold tolerance in cypress (Cupressus sempervirens L.): a physiological and molecular study. Tree Genetics Genomes, 7, 79-90.
  6. Baninasab, B. (2009). Amelioration of chilling stress by pa­clobutrazol in watermelon seedlings. Scientia Horticulture, 121, 144-148.
  7. Banon, S., Ochoa, J., Franco, J. A, Sanchez-Blanco, M. J. & Alarcon, J. J. (2003). Influence of water deficit on low air humidity in the nursery on survival of Rhannus alaternus seedlings following planting. Journal of Horticultural Science and Biotechnolgy, 78, 518-522.
  8. Behra, R. K., Mishra, P. & Choudhury, N. K. (2002). High irradiance and water stress induce alterations in pigment composition and chloroplast activities of primary wheat leaves. Journal of Plant Physiology, 159, 967-973.
  9. Berova, M., Zlatev, Z. & Stoeva, N. (2002). Effect of paclobutrazol on wheat seedlings under low temperature stress. Bulgarian Journal of Plant Physiology, 28, 75-84.
  10. Cayuela, E., Munoz-Mayor, A., Vicente-Agullo, F., Moyano, E., Garcia-Abellan, J. O., Estan, M. T. & Bolarin, M. C. (2007). Drought pretreatment increases the salinity resistance of tomato plants. Journal of Plant Nutrition and Soil Science, 170, 479-484.
  11. Chandra Rai, A., Singh, M. & Shah, K. (2013). Engineering drought tolerance tomato plants over-expressing BcZAt12 gene encoding aC2H2 zinc finger transcription factor. Phytochemistry, 85: 44-50.
  12. Dat, J. V. S., Vranova, E. & Van Montagu, M. (2000). Dual action of the active oxygen species during plant stress esponses. Cell Molecule Life Science, 57(5), 779-795.
  13. Dong, X., Bi, H., Wu, G. & Ai, X. (2013). Drought-induced chilling tolerance in cucumber involves membrane stabilisation improved by antioxidant system. International Journal of Plant Production, 7(1), 67-79.
  14. Hallgreen, J. E. & Öquest, G. (1990). Adaptations to low temperatures. Stress responses in plants: Adaptation and acclimation mechanisms. Wiley-Liss Inc. New York, NY. 265-293.
  15. Helmy, Y. I., Singer, S. M. & El-Abd, S. O. (1999). Reduction chilling injury by short term cold acclimation of cucumber seedlings under protected cultivation. Acta Horticulturae, 491: 177-184.
  16. Hura, K., Hura, T., Rapacz, M. & Pazek, A. (2016). Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans. Biologia, 70(8), 1011-1018.
  17. Joshi, S. C., Chandra, S. & Palni, L. M. S. (2007). Differences in photosynthetic characteristics and accumulation of osmoprotectants in saplings of evergreen plants grown inside and outside a glasshouse during the winter season. Photosynthetica, 45, 594-600.
  18. Krasensky, J. & Jonak, C. (2012). Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. Journal of Experimental Botany, 63, 1593-1608.
  19. Liptay, A., sikkema, P. & Fonteno, W. (1998). Transplant growth control through water deficit stress.  Horticulture Technology, 8, 540-543.
  20. Maali-Amiri, R. & Goldenkova-Pavlova, I. V. (2007). Lipiid fatty acid composition of potato plants transformed with delta 12-desaturase gene from cyano-bacterium. Russian Journal of Plant Physiology, 54(5), 678-685.
  21. Maxwell, K. & Johnson, G. N. (2000). Chlorophyll fluorescence-a practical guide. Journal of Experimental Botany, 51(345), 659-668.
  22. McDonald, S., Prenzler, P. D., Autolovich, M. & Robards, K. (2001). Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73, 73-84.
  23. Pardossi, F., Tognoni, F. & Lovemore, S. S. (1988). The effect of different hardening treatments on tomato seedling growth, Chilling resistance and crop production in cold greenhouse. Acta Horticulturae, 229, 371-379.
  24. Qiujie, D., Bin, Y. S., Xiao, Z. & Wang, Z. (1996). Flooding- induce memberance damage, lipid oxidation and activated oxygen generation in corn leaves. Plant and Soil, 179, 261-268.
  25. Saltveit, M. E. (2000). Chilling injury is reduced in cucumber and rice seedlings and in tomato pericarp discs by heat-shocks applied after chilling. Postharvest Biology and Technolog, 21, 169-177.
  26. Sato, F., Yoshioka, H., Fujiwara, T., Higashio, H., Uragami, A. & Tokuda, S. (2004). Physiological responses of cabbage plug seedlings to water stress during low-temperature storage in darkness. Scientia Horticulturae, 101, 349-357.
  27. Schutz, M. & Fangmeir, E. (2001) Growth and yield response of spring wheat (Triticum aestivum L. cv. Minaret) to elevated CO2 and water limitation. Environmental Pollution, 11, 187-194.
  28. Stewart, R. R. C. & Bewley, J. D. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology, 65, 245-248.
  29. Strain, H. H. & Svec, W. A. (1966). Extraction, separation and iso­lation of chlorophylls. Chlorophylls. Academic Press, New York. 24-61 pages.
  30. Wang, C. Y. (1990). Alleviation of chilling injury of horticultural crops. CRC Press, 313 pages.