Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

The effect of different concentrations of benzylaminopurine and naphthalene acetic acid on direct regeneration of explants of four Persian shallot (Allium hirtifolium) ecotypes at in vitro condition

Document Type : Full Paper

Authors
Nasrin Farhadi 1
Jaber Panahandeh 2
Alireza Motallebi Azar 2
Saeideh Alizadeh Salteh 3
1 Ph. D. Candidate, Faculty of Agriculture, University of Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Iran
3 Assistant Professor, Faculty of Agriculture, University of Tabriz, Iran
10.22059/ijhs.2017.201336.946
Abstract
In the present study, the effects of different concentrations of benzylamino purine (0, 1, 1.5 and 2 mg l-1) and naphthalene acetic acid (0, 0.25, 0.5 and 1 mg l1-) on direct and indirect bulblet rejected set by user of basal plate explants of four ecotypes of Psersian shallot (Lorestan, Zanjan, Sanandaj and Arak) on MS medium were investigated. The results showed that different hormonal treatments and ecotypes affected the time and percent of bulblet regeneration as well as the number, length and weight of regenerated bulblets. Moreover, direct and indirect regeneration of bulblet were different among the treatments. Application of BAP and NAA accelerated the bulblet regeneration of Persian shallot explants and the highest bulblet regeneration (82.26%) and maximum bulbllets number (14.13) were obtained by 1.5 mg g-1 BAP with 0.5 mg l-1 NAA. The explants of Arak ecotype showed the highest percent of bulblet regeneration (64.61%) and bulblets number (10.33) per each explant. The callus induction was only observed in 2 mg l-1 BAP+1 mg l-1 NAA (75.05%) and 1 mg l-1 BAP+0.5 mg l-1 NAA (23.67%) treatments. The rooting of bulblet in all the studied ecotypes was obtained in hormone-free medium in the shortest time (25 after culture) in comparison with other treatments that was also accompanied with the highest percent of rooting (79.917%). According to the results the culture of Arak ecotype bulbs in medium with 1.5 mg g-1 BAP and 0.5 mg l-1 NAA is advisable for invitro bulblets production of Persian shallot.
Keywords
Auxin
bulblet regenerstion
cytokinin
ectype
mooseer
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Iranian Journal of Horticultural Science
Volume 48, Issue 3 - Serial Number 3
Winter 2018
Pages 517-526

  • Receive Date 14 March 2016
  • Revise Date 30 April 2016
  • Accept Date 03 May 2016