The effect of carboxymethyl cellulose and pistachio (Pistacia atlantica L.) essential oil coating on fruit quality of cold-stored grape cv. Rasheh

Document Type : Full Paper


1 Associate Professor, Department of Horticultural Sciences, Faculty of Agricultural, University of Kurdistan, Sanandej, Iran. P. O. Box: 416, Postal code: 66177-15175

2 Former M. Sc. Student, Department of Horticultural Sciences, Faculty of Agricultural, University of Kurdistan, Sanandej, Iran. P. O. Box: 416, Postal code: 66177-15175


Application of natural compounds such as essential oils and plant materials as bio-products is known as an appropriately safe strategy for controlling decay and maintaining fruit quality. Pistachia atlantica essential oil (PAO) (ranging between 0, 200, 400, 600, 800, 1000 and 1200 µl l-1) was tested for antifungal activity against Botrytis cinerea in vitro conditions. Carboxymethyl cellulose (CMC) (0, 1 and 2%) and PAO (0, 600 and 1200 µl l-1) were applied to the "Rasheh" grope for improving grape cold storage to preserve fresh fruit quality during cold stored postharvest. The growth of B. cinerea mycelia was greatly inhibited up to 84% at 1200 µl l-1 PAO under in vitro conditions. CMC and PAO treatments led to delaying grape weight loss and fruit decay. Titratable acidity, anthocyanin, antioxidant capacity, phenol and tannin of uncoated fruits decreased and total soluble solid contents increased during the grape storage. Fruit coating with CMC and PAO maintained higher anthocyanin, antioxidant capacity, phenol, tannin and titratable acidity. Less increase of total soluble solids was observed in coated fruits when compared to control fruits. The results indicated the beneficial effect of CMC (2%) and PAO (1200 µl l-1) coating on maintaining of grape fruit quality 28 d after storage.


Main Subjects

  1. Abdolahi, A., Hassani, A., Ghosta, Y., Javadi, T. & Meshkatalsadat, M. H. (2010). Essential oils as control agents of postharvest Alternaria and Penicillium rots on tomato fruits. Journal of Food Safety, 30, 341-352.
  2. Aloui, H., Khwaldia, K. H., Sa´nchez-Gonza´ lez, L., Muneret, L., Jeandel, C., Hamdi, M. & Desobry, S. (2014). Alginate coatings containing grapefruit essential oil or grapefruit seed extract for grapes preservation. International Journal of Food Science & Technology, 49, 952-959.
  3. Antunes, M. D. C. & Cavaco, A. M. (2010). The use of essential oils for postharvest decay control. A review. Flavour Fragrance Journal, 25, 351-366.
  4. Baar, A. & Kulicke, W. M. (1994). Nuclear magnetic resonance spectroscopic characterization of carboxymethyl cellulose. Macromolecular Cellular Physics, 195, 1483-1492.
  5. Baiano, A. & Terracone, C. (2011). Varietal differences among the phenolic profiles and antioxidant activities of seven table grape cultivars grown in the south of Italy based on chemometrics. Journal of Agricultural & Food Chemistry, 59, 9815-9826.
  6. Barman, K., Asrey, R., Pal R. K., Kaur, C. & Jha, S. K. (2014). Influence of putrescine and carnauba wax on functional and sensory quality of pomegranate (Punica granatum L.) fruits during storage. Journal of Food Science & Technology, 51(1), 111-117.
  7. Bello, F. A. & Henry, A. A. (2015). Storage effects and the postharvest quality of African star apple fruits (Chrysophyllum africanum) under ambient conditions. African Journal of Food Science & Technology, 6(1), 35-43.
  8. Castillo, S., Navarrob, D., Sparta, P. J., Guilléna, F., Valeroa, D., Serranob, M. & Martínez-Romero, D. (2010). Antifungal efficacy of Aloe vera in vitro and its use as a preharvest treatment to maintain postharvest table grape quality. Postharvest Biology & Technology, 57, 183-188.
  9. Cha, D. S. & Chinnan, M. (2004). Biopolymer-based antimicrobial packaging: a review. Critical Reviews in Food Science & Nutrition, 44, 223-237.
  10. Chervin, C., Lavigne, D. & Westercamp, P. (2009). Reduction of gray mold development in table grapes by preharvest sprays with ethanol and calcium chloride. Postharvest Biology & Technology, 54, 115-117.
  11. Conde, C., Silva, P., Fontes, N., Dias, A. C. P., Tavares, R. M., Sousa, M. J., Agasse, A., Delrot, S. & Gerós, H. (2007). Biochemical changes throughout grape berry development, fruit, and wine quality. Food Global Science Books, 1(1), 1-22.
  12. Crisosto, C. H., Garner, D. & Crisosto, G. (2002). Carbon dioxide-enriched atmospheres during cold storage limit losses from Botrytis but accelerate rachis browning of ‘Redglobe’ table grapes. Postharvest Biology & Technology, 26, 181-189.
  13. Cristescu, S. M., De Martinis, D., Hekkert, S. L., Parker, D. H. & Harren, F. J. M. (2002). Ethylene production by Botrytis cinerea in vitro and in tomatoes. Applied and Environmental Microbiology, 68, 5342-5350.
  14. Delazar, A., Reid, G. & Sarker, D. (2004). GC-MS Analysis of the essential oil from oleoresin of Pistacia atlantica VAR. mutica. Chemistry of Natural Compounds, 40(1), 24-27.
  15. Díaz-Mula, H. M., Serrano, M. & Valero, D. (2012). Alginate Coatings Preserve Fruit Quality and Bioactive Compounds during Storage of Sweet Cherry Fruit. Food Bioprocess Technology, 5, 2990-2997.
  16. Elmer, P. A. G. & Reglinski, T. (2006). Biosuppression of Botrytis cinerea in grapes. Plant Pathology, 55, 155-177.
  17. Ferreyra, R. M., Vi˜na, S. Z., Mugridge, A. & Chaves, A. R. (2007). Growth and ripening season effects on antioxidant capacity of strawberry cultivar Selva. Scientia Horticulturae, 112, 27-32.
  18. Gol, N. B., Patel, R. & Rao, T. V. R. (2013). Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan. Postharvest Biology & Technology, 85, 185-195.
  19. Gourine, N., Yousfi, M., Bombarda, I., Nadjemi, B. & Gaydou, E. (2010). Seasonal variation of chemical composition and antioxidant activity of essential oil from Pistacia atlantica Desf. Leaves. Journal of the American Oil Chemists' Society, 87, 157-166.
  20. Guillén, F., Zapata, P. J., Martinez-Romero, D., Castillo, S., Serrano, M. & Valero, D. (2007). Improvement of the overall quality of table grapes stored under modified atmosphere packaging in combination with natural antimicrobial compounds. Journal of Food Science, 72, 185-190.
  21. Hassani, A., Fathi, Z., Ghosta, Y., Abdollahi, A., Meshkatalsadat, M. H. & Jalili Marandi, R. (2012). Evaluation of plant essential oils for control of postharvest brown and gray mold rots on apricot. Journal of Food Safety, 32, 94-101.
  22. Hatamnia, A. A., Abbaspour, N. & Darvishzadeh, R. (2014). Antioxidant activity and phenolic profile of different parts of Bene (Pistacia atlantica subsp. kurdica) fruits. Food Chemistry, 145, 306-311.
  23. Hesami, G., Hesami, S. & Fatemi, A. (2013). Effect of Pistacia atlantica Subsp. Kurdica essential oil and acetic acid on Botrytis cinerea growth in culture media, grape and cucumber fruits. International Journal of Microbiology & Mycology, 1(2), 13-21.
  24. Hussain, P. R., Suradkar, P. P., Wani, A. M. & Dar, M. A. (2015). Retention of storage quality and post-refrigeration shelf-life extension of plum (Prunus domestica L.) cv. Santa Rosa using combination of carboxymethyl cellulose (CMC) coating and gamma irradiation. Radiation Physics & Chemistry, 107, 136-148.
  25. Hussain, P. R., Rather, S. A., Suradkar, P., Parveen, S., Mir, M. A. & Shafi, F. (2016). Potential of carboxymethyl cellulose coating and low dose gamma irradiation to maintain storage quality, inhibit fungal growth and extend shelf-life of cherry fruit. Journal of Food Science and Technology, 53(7), 2966-2986.
  26. Jiang, Y. & Li, Y. (2001). Effects of chitosan coating on postharvest life and quality of longan fruit. Food Chemistry, 73, 139-143.
  27. Jin, P., Wang, S. Y., Gao, H., Chen, H., Zheng, Y. & Wang, C. Y. (2012). Effect of cultural system and essential oil treatment on antioxidant capacity in raspberries. Food Chemistry, 132, 399-405
  28. Khademi, O., Zamani, Z., Poor Ahmadi, E. & Kalantari, S. (2013). Effect of UV-C radiation on postharvest physiology of persimmon fruit (Diospyros kaki Thunb.) cv. `Karaj´ during storage at cold temperature. International Food Research Journal, 20(1), 247-253.
  29. Kulkarni, A. P. & Aradhya, S. M. (2005). Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93, 319-324.
  30. Liu, J., Tian, S. P., Meng, X. H. & Xu, Y. (2007). Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit. Postharvest Biology & Technol, 44, 300-306.
  31. Lutz, M., Jorquera, K., Cancino, B., Ruby, R. & Henriquez, C. (2011). Phenolics and antiox-idant capacity of table grape (Vitis vinifera L.) cultivars grown in Chile. Journal of Food Science, 76, 1088-1093.
  32. Maftoonazad, N., Ramaswamy, H. S. & Marcotte, M. (2008). Shelf-life extension of peaches through sodium alginate and methyl cellulose edible coating. International Journal of Food Science & Techology, 43, 951-957.
  33. Malmiri, H. J., Osman, A., Tan, C. P. & Rahman, A. R. (2011). Evaluation of effectiveness of three cellulose derivative-based edible coatings on changes of physico-chemical characteristics of ‘Berangan’ banana (Musa sapientum cv. Berangan) during storage at ambient conditions. International Food Research Journal, 18, 1381-1386.
  34. Maqbool, M., Ali, A. & Alderson, P. G. (2010). Effect of cinnamon oil on incidence of anthracnose disease and postharvest quality of bananas during storage. International Journal of Agriculture Biology, 12, 516-520.
  35. Marino, M., Bersani, C. & Comi, G. (2001). Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International Journal of Food Microbiology, 67, 185-187.
  36. Melgarejo-Flores, B. G., Ortega-Ramírez, L. A., Silva-Espinoza, B. A., González-Aguilar, G. A., Miranda, M. R. A. & Ayala-Zavala, J. F. (2013). Antifungal protection and antioxidant enhancement of table grapestreated with emulsions, vapors, and coatings of cinnamon leaf oil. Postharvest Biology & Technology, 86, 321-328.
  37. Meng, X., Li, B., Liu, J. & Tian, S. 2008. Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chemistry, 106, 501–508.
  38. Mónica, G.M. & Wrolstad, R.E. 2001. Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy. Current
    Protocols in Food Analytical Chemistry, F1.2.1-F1.2.13.
  39. Nordin, M.A.F., Harun, W. H. A.W. & Razak, F. A. 2013. Antifungal susceptibility and growth inhibitory response of oral Candida species to Brucea javanica Lin. Extract. BMC Complementary & Alternative Medicine, 13, 342-350.
  40. Pastor, C., Sánchez-González, L., Marcilla, A., Chiralt, A., Chafer, M. & Gonzalez-Martinez, C. (2011). Quality and safety of table grapes coated with hydroxypropylmethylcellulose edible coatings containing propolis extract. Postharvest Biology & Technology, 60, 64-70.
  41. Plotto, A., Roberts, R. G. & Roberts, D. D. (2003). Evaluation of plant essential oils as natural postharvest disease control of tomato (Lycopersicum esculentum). Acta Horticulturae, 628,737-745.
  42. Ramezani, H., Singh, H. P., Batish, D. R., Kohli, R. K. & Dargan, J. S. (2002). Fungicidal effect of volatile oils from Eucalyptus citriodora and its major constituent citronellal. New Zealand Plant Protection, 55, 327-330.
  43. Sánchez-González, L., Vargas, M., González-Martínez, C., Chiralt, A. & Cháfer, M. (2009). Characterization of edible films based on hydroxypropylmethylcellulose and tea tree essential oil. Journal of Food Hydrocolloid, 23, 2102-2109.
  44. Sánchez-González, L., González-Martínez, C., Chiralt, A. & Cháfer, M. (2010a). Physical and antimicrobial properties of chitosan-tea tree essential oil composite films. Journal of Food Engineering, 98, 443-452.
  45. Sánchez-González, L., Cháfer, M., Chiralt, A. & González-Martínez, C. (2010b). Physical properties of edible chitosan films containing bergamot essential oil and their inhibitory action on Penicillium italicum. Carbohydrate Polymers, 82, 277-283.
  46. Sánchez-González, L., Pastor, C., Vargas, M., Chiralt, A., González-Martínez, Ch. & Cháfer, M. (2011a). Effect of hydroxypropylmethylcellulose and chitosan coatings with and without bergamot essential oil on quality and safety of cold-stored grapes. Postharvest Biology & Technology, 60, 57-63.
  47. Sánchez-González, L., Vargas, M., Gonzalez-Martinez, C., Chiralt, A. & Chafer, M. 2011b. Use of essential oils in bioactive edible coatings: a review. Food Engineering Review, 3, 1-16.
  48. Sellamuthu, P. S., Sivakumar, D., Soundy, P. & Korsten, L. (2013). Enhancing the defence related and antioxidant enzymes activities in avocado cultivars with essential oil vapours. Postharvest Biology & Technology, 81, 66-72.
  49. Serrano, M., Martinez-Romero, D., Castillo, S., Guillen, F. & Valero, D. (2005). The use of the natural antifungal compounds improves the beneficial effect of MAP in sweet cherry storage. Innovative Food Science & Emerging Technologies, 6, 115-123.
  50. Shiri, M. A., Ghasemnezhad, M., Bakhshi, D. & Sarikhani, H. (2013). Effect of postharvest putrecine application and chitosan coating on maintaining quality of table grape “Shahroudi” during long-term storage. Journal of Food Processing & Preservation, 37, 999-1007.
  51. Slinkard, K. & Singleton, V. L. (1977). Total phenol analyses: automation and comparison with manual methods. American Journal of Enology & Viticulture, 28, 49-55.
  52. Talibi, I., Askarne, L., Boubaker, H., Boudyach, E. H., Msanda, F., Saadi, B. & Ait Ben Aoumar, A. (2012). Antifungal activity of Moroccan medicinal plants against citrus sour rot agent Geotrichum candidum. Letters in Applied Microbiology, 55, 155-161.
  53. Tanović, B., Hrustić, J., Mihajlović, M., Grahovac, M. & Delibašić, G. (2015). Botrytis cinerea in raspberry in Serbia II: Growth rate and virulence of isolates. Pesticides & phyttomedicine (Belgrade), 30(1), 9-16.
  54. Taira, S., Ono, M. & Matsumoto, N. (1997). Reduction of persimmon astringency by complex formation between pectin and tannin. Postharvest Biology & Technology, 12, 265-271.
  55. Tian, J., Ban, X. Q., Zeng, H., He, J. S., Huang, B. & Wang, Y. W. (2011). Chemical composition and antifungal activity of essential oil from Cicuta virosa L. var. latisecta Celak. International Journal of Food Microbiology, 145, 464-470.
  56. Tien, C. L., Letendre, M., Ispas-Szabo, P., Mateescu, M. A., Patterson, G. D., Yu, H. L. & Lacroix, M. (2000). Development of biodegradable films from whey proteins by cross-linking and entrapment in cellulose. Journal of Agricultural & Food Chemistry, 48, 5566-5575.
  57. Toivonen, P. M. A. (2007). Fruit maturation and ripening and their relationship to quality. Stewart Postharvest Review, 3(2), 1-7.
  58. Togrul, H. & Arslan, N. (2003). Production of carboxymethyl cellulose fromsugar beet pulp cellulose and rheological behaviour of carboxymethyl cellulose. Carbohydrate Polymers, 54, 73-82.
  59. Tzortzakis, N. G. (2009). Impact of cinnamon oil-enrichment on microbial spoilage of fresh produce. Innovative Food Science & Emerging Technologies, 10, 97-102.
  60. Ultee, A., Kets, E. P. W. & Smid, E. J. (1999). Mechanisms of action of cavacrol on the food-borne pathogen Bacillus cereus. Applied & Environmental Microbiology, 65, 4606-4610.
  61. Valencia-Chamorro, S. A., Pérez-Gago, M. B., Del Rio, M. A. & Palou, L. (2009). Effect of antifungal hydroxypropylmethylcellulose (HPMC)–lipid edible composite coatings on postharvest decay development and quality attributes of cold-stored Valencia oranges. Postharvest Biology & Technology, 54, 72-79.
  62. Valero, D., Valverde, J. M., Martínez-Romero, D., Guillén, F., Castillo, A. & Serrano, M. (2006). The combination of modified atmosphere packaging with eugenol or thymol to maintain quality, safety and functional properties of table grapes. Postharvest Biology & Technology, 41, 317-327.
  63. Wang, C. Y. (2003). Maintaining postharvest quality of raspberries with natural volatile compounds. International Journal of Food Science & Technology, 38, 869-875.
  64. Wang, S. Y. & Gao, H. (2013). Effect of chitosan-based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria x aranassa Duch.). LWT-Food Science & Technology, 52, 71-79.
  65. Xia, Z., Mao, X. & Luo, Y. (1999). Study on antifungal mechanism of alpha- pinene. Hunan Yi Ke da Xue Xue Bao, Bulletin of Hunan Medical University, 24(6), 507-509.
  66. Xing, Y., Li, X., Xu, Q., Yun, J. & Lu, Y. (2010). Antifungal activities of cinnamon oil against Rhizopus nigricans, Aspergillus flavus and Penicillium expansum in vitro and in vivo fruit test. International Journal of Food Science & Technology, 45, 1837-1842.
  67. Yaman, Ö. & Bayındırl, L. (2001). Effects of an edible coating, fungicide and cold storage on microbial spoilage of cherries. European Food Research & Technology, 213, 53-55.
  68. Yinzhe, R. & Shaoying, Z. (2013).Effect of Carboxymethyl Cellulose and Alginate Coating Combined with Brewer Yeast on Postharvest Grape Preservation. Hindawi PublishingCorporation., 1-7.
  69. Youssef, K. & Roberto, S. R. (2014). Applications of salt solutions before and after harvest affect thequality and incidence of postharvest gray mold of ‘Italia’ table grapes. Postharvest Biology & Technology, 87, 95-102
  70. Zhou, R., Mo, Y., Li, Y., Zhao, Y., Zhang, G. & Hu, Y. (2008). Quality and internal characteristics of Huanghua pears (Pyrus pyrifoliaNakai, cv. Huanghua) treated with different kinds of coatings during storage. Postharvest Biology & Technology, 49, 171-179.
  71. Ziedan, E. H. E. & Farrag, E. S. H. (2008). Fumigation of peach fruits with essential oils to control postharvest decay. Research journal of agriculture & biological sciences, 4, 512-519.
  72. Zokaee Khosroshahi, M. R., Esna-Ashari, M. & Ershadi, A. (2007). Effect of exogenous putrescine on postharvest life of strawberry (Fragaria ananassa Duch.) fruit, cultivar Selva. Scientia Horticulturae, 114, 27-32.