In vitro propagation of Persian lime (Citrus latifolia)

Document Type : Full Paper


Assistant Professor, Agriculture Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, Karaj, Iran


Application of tolerant and resistant rootstocks and varieties is the best way to overcome Witches’ Broom Disease of Lime (WBDL). The aim of this research was to develop a practical protocol for micropropagtaion of Persian lime (Citrus latifolia) as one of the highly tolerant varieties. For culture establishment, apical and axillary buds of young stems of three-year-old trees were cultured in free hormone MS medium after surface sterilization. The explants were transferred to DKW and MS culture media containing different concentrations of BAP for proliferation after 20-30 days. Another experiment was carried out using Kinetin and BAP for determination of shoot elongation medium. Two separated experiments using three concentrations of IAA and NAA and three concentrations of IBA and NAA in DKW medium and 2% sucrose were used to obtain the best medium for rooting. To acquire the suitable soil mixture for acclimatization, an experiment using six different soil mixtures was done. The results showed that DKW basal culture medium supplemented with Gamborge’s B5 vitamins, 1 mg.L-1 BAP and 3% sucrose was the best medium for proliferation. DKW basal culture medium supplemented with Gamborge’s B5 vitamins and 0.25 mg.L-1 BAP was the best medium for elongation. The best rooting medium was DKW basal culture medium, Gamborge’s B5 vitamins, 2% sucrose and 2 mg. L-1 NAA. The optimum soil mixture with 88% of survival for acclimating in vitro plantlets was sand: perlite: peat: clay (1: 1: 3: 3). The plantlets could transfer to the orchard after 60 days.


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