Evaluation of genetic stability of plantlets from tissue culture Date Palm using microsatellite markers

Document Type : Full Paper


1 Assistance Professor, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran

2 Assistant Professor, Faculty of Agriculture, Ramin Agriculture and Natural Resources of Khouzestan, Iran

3 Former Ph.D. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

4 Former M. Sc. Student, Faculty of Agriculture, Ramin Agriculture and Natural Resources of Khouzestan, Iran

5 Assistant Professor, Research Institute for Biotechnology and Bio Engineering, Isfahan University of Technology, Isfahan, Iran


Date palm (Phoenix dactylifera L.) is a dioecious and long life plant which has high economic important in Iran. Date palm is propagated traditionally through the off shoot that is more laborious and expensive. So, other amplification methods, such as tissue culture are used for its multiplication. Plant tissue culture is leading to somaclonal variation, due to epigenetic non- inherited variation which is a temporary phenotypic effect.  However, in some cases, these genetic instability caused reduction at the tendency of farmers to cultivate tissue- culture based genotypes of palm. Genetic stability of ten Iranian genotypes of date palm (originated by tissue culture via direct organogenesis and off-shoot) was evaluated by 20 primers of SSR (Simple Sequence Repeat). Four primers (mPdCIR044, PDAAG1023, DP172 and PDAAG1025) were polymorphic. According to obtained results, total of 38 alleles were detected, resulting a mean number of 1.9 allele per locus. No microsatellite DNA variation was observed among tissue culture and their offshoots in each cultivar. According to WARD cluster analysis, the genotypes were grouped into two main groups. The results of this study showed that identical genetic structure for date palm originated by two methods of tissue culture and off-shoot, in each cultivar. Hence, the plant tissue culture method is advised for rapid propagation in date palm genotypes. 


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