Effect of hydrogen peroxide treatment on improvement of the postharvest quality of cut Alstroemeria cut flowers

Document Type : Full Paper


1 M. Sc. Students, Associated Professor and Former M. Sc. Student, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran

2 Associated Professor, Faculty of Science, Shahid Bahonar University of Kerman, Iran

3 Associated Professor, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran

4 Former M. Sc. Student, Faculty of Science, Shahid Bahonar University of Kerman, Iran


In this study a CRD experiment with three replications was carried out to assess the effect of different concentration of hydrogen peroxide (H2O2) (200, 400 and 600 µM) on the improvement of vase life and postharvest quality of cut Alstroemeria flowers. All of applied hydrogen peroxide concentration increased the vase life of cut flowers. The highest vase-life was obtained in 600 µM H2O2 which increased vase-life for 7 extra days in comparison with control flowers. Hydrogen peroxide also decreased the floral wilting, MDA content and water loss of flowers compared to control flower, while increased the ascorbate peroxidase and guaiacol peroxidase enzymes activity. Catalase activity increased significantly only in 600 µM H2O2 treatment. Polyphenol oxidase activity assay in petal showed that hydrogen peroxide decreased the activity of this enzyme significantly in comparison with control flowers. Data of this research showed that the application of exogenous hydrogen peroxide in optimal concentration act as signal molecule and through decrease of lipid peroxidation,  induction of the antioxidant enzymes activity and the inhibition of polyphenol oxidase activity increased the vase life and postharvest quality of cut Alstroemeria flowers.


  1. Agarwal, S., Sairam, R. K., Srivastava, G. C., Tyagi, A. & Meena, R. C.  (2005). Role of ABA, salicylic acid, calcium and hydrogen peroxide on antioxidant enzymes induction in wheat seedlings. Plant Science, 169, 559-570.
  2. Ashfaque, F., Iqbal, M., Khan, R. & Khan, N. (2014). Exogenously applied H2O2 promotes proline accumulation, water relations, photosynthetic efficiency and growth of wheat (Triticum aestivum L.) under salt stress. Annual Research & Review in Biology, 4, 105-120.
  3. Beligni, M. V. & Lamattina, L. (1999). Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues. Planta, 208, 337-344.
  4. Bienert, G. P., Schjoerring, J. K. & Jahn, T. P. (2006). Membrane transport of hydrogen peroxide. Biochemistry and Biophysic Acta, 1758, 994-1003.
  5. Bradford, M. N. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochemistry, 72, 248-254.  
  6. Buchanan-Wollaston, V., Earl, S., Harrison, E., Mathas, E., Navab-pour, S., Page, T. & Pink, D.  (2003). The molecular analysis of plant senescence a genomics approach. Plant Biotechnology Journal, 1, 3-22.
  7. Chen, H., McCarig, B., Melotto, M., Yang He, S. & Howe, G. A. (2004). Regulation of plant arginase by wounding, Jasmonate and the phytotoxin coronatine. Journal of Biological Chemistry, 279, 45998-46007.
  8. Delauney, A. J. & Verma, D. P. (1993). Proline biosynthesis and degradation in plants. Plant Journal, 4, 215-223.
  9. Dhindsa, R. S., Dhinds, D. & Thorpe, T. A. (1981). Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32, 93-101.
  10. Emongor, V. E. (2004). Effects of gibberellic acid on post-harvest quality and vase life of gerbera cut flowers (Gerbera jamesonnii). Agronomy, 3, 191-195.
  11. Farooq, S. & Azam, F. (2006). The use of cell membrane stability (CMS) technique to screen for salt tolerant wheat varieties. Journal of Plant Physiology, 163, 629-637.
  12. Fu, J. & Huang, B. (2001). Involvement of antioxidant and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany, 45, 105-114.
  13. Ghasemi Ghahsare, M. & Kafi, M. (2005). Scientific and practical floriculture. Publications Golbon Isfahan, Iran. 312 p. (in Farsi)
  14. Gondim, F., Gomes-Filho, E., Lacerda, C., Prisco, Tarquinio, J., Azevedo Neto, A. & Marques, E. (2010). Pre-treatment with H2O2 in maize seeds: effects on germination and seedling acclimation to salt stress.Brazilian Journal of Plant Physiology, 22(2), 103-112
  15. He, L., Gao, Z. & Li, R. (2009). Pretreatment of seed with H2O2 enhances drought tolerance of wheat (Triticum aestivum L.) seedlings. African Journal of Biotechnology, 8, 6151-6157.
  16. Heath, R. L. & Packer, L. (1968). Photoperoxidation in isolated chloroplast, kinetics and stoichiometry of fatty acid peroxidation. Journal of Archive Biochemistry and Biophysics, 125, 189-198.
  17. Huang, K. L. & Chen, W. S. (2002). BA and sucrose increase vase life of cut Eustoma flower. Horticultural Science, 37, 547-549.
  18. Kumar, N., Pal, M., Singh, A., Sairam, R. & Srivastava, G. C. (2010). Exogenous proline alleviates oxidative stress and increase vase life in rose (Rosa hybrida L. ‘Grand Gala’). Scientia Horticulturae, 127, 79-85.
  19. Li, J. T., Qui, Z. B., Zhang, X. W. & Wang, L. S. (2011). Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress. Acta Physiologia Plantarum, 33, 835-842.
  20. Liao, W., Zhang, M., Huang, G. & Yu, J. (2012). Hydrogen peroxide in the vase solution increases vase life and keeping quality of cut oriental × trumpet hybrid lily ‘Manissa’. Scientia Horticulturae,  139, 32-38.
  21. Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Sciences, 7, 405-410.
  22. Mostofi, Y. P., Rasooli, R., Naderi, Gh., Marandi, B. & Shafiee, M. (2010). Study the effect of nitric oxide and tidiazorone on vase life and some quality characteristics of Dianthus cut flower. Horticultural Journal, 41, 310-308. (in Farsi)
  23. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach choloroplast. Plant Cell Physiology, 22, 867-880.
  24. Nasibi, F. (2011). Effect of different concentrations of sodium nitroprusside (SNP) pretreatment on oxidative damages induced by drought stress in tomato plant. Journal of Plant Biology, 9, 63-74. (in Farsi)
  25. Nicoli, M. C., Elizable, B. E., Piotti, A. & Lerici, C. R. (1991). Effect of sugar and maillard reaction products on polyphenol oxidase and peroxidase activity in food. Journal of Food Biochemistry, 15, 169-184.
  26. Plewa, M. J., Smith, S. R. & Wanger, E. D. (1991). Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Research, 247, 57-64.
  27. Serek, M. & Reid, M. S. (1997). Use of growth regulators for improving the postharvest quality of ornamentals. Perishables Handling, 92, 7-8.
  28. Shimizu-Yumoto, H. & Ichimura, K. (2009). Abscisic acid, in combination with sucrose, is effective as a pulse treatment to suppress leaf damage and extend foliage vase-life in cut Eustoma flowers. Journal of Horticultural Sciences and Biotechnology, 84, 107-111.
  29. Singh, A., Kumar, J. & Kumar, P.  (2008). Effects of plant growth regulators and sucrose on post harvest physiology, membrane stability and vase life of cut spikes of gladiolus. Plant Growth Regulation, 55, 221-229.
  30. Ślesak, I., Libik, M., Karpinska, B., Karpinski, S. & Miszalski, Z. (2007). The role of hydrogen peroxide in regulation of plant metabolism and cellular signaling in response to environmental stresses. Acta Biochemical, 54, 39-50.
  31. Smirnoff, N. (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytology, 125, 27-58.
  32. Tian, X. & Li, Y. (2006). Nitric oxide treatment alleviates drought stress in wheat seedlings. Biologia Plantarum, 50, 775-778.
  33. Vranová, E., Inzé, D. & Van Breusegem, F. (2002). Signal transduction during oxidative stress. Journal of Experimental Botany, 53, 1227-1236.
  34. Wahida, A., Perveena, M., Gelania, S. & Basrab, S. (2007). Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology, 164, 283-290.
  35. Youssefian, S., Nakamura, M., Orudgev, E. & Kondo, N. (2001). Increased cysteine biosynthesis capacity of transgenic tobacco over expressing an O-acetylserine (thiol) lyase modifies plant responses to oxidative stress. Plant Physiology, 126, 1001-1011.
  36. Zamani, S., Kazemi, M. & Aran, M. (2011). Postharvest life of cut rose flowers as affected by salicylic acid and glutamin. World Applied Sciences Journal, 12, 1621-1624.
  37. Zhang, H., Hu, S. L., Zhang, Z. J., Hu, L. Y., Jiang, C. X., Wei, J., Liu, J., Wang, H. L. & Jiang, S. T.  (2011). Hydrogen sulfide acts as a regulator of flower senescence in plants. Postharvest Biology and Technology, 60, 251-257.