The effect of substrate and composting duration on biological efficiency and quality properties of Button Mushroom (Agaricus bisporus)

Document Type : Full Paper


1 Former M. Sc. Student, Faculty of Agricultural Sciences, University of Guilan, Iran

2 Assistant Professor, Faculty of Agricultural Sciences, University of Guilan, Iran

3 Instructor, Rice Research Institute of Iran


In order to evaluate the effect of substrate and composting duration on biological efficiency and improve some quality properties of button mushroom (Agaricus bisporus) at different substrates from agricultural waste, a factorial experiment in a completely randomized design was done with twelve treatment combinations (six substrates and two composting durations) with three replications. Treatments used in the experiment consisted of substrates including wheat straw and rice straw as the main material and chicken manure, olive bagasse and horse manure as additives, and two composting durations (short (16 days) and long (22 days)). Substrate prepared from rice straw and chicken manure had the highest biological efficiency (67.18 %). Substrate prepared from rice straw and olive bagass had the highest dry matter (8.73 %), while mushrooms obtained from substrate prepared from wheat straw and olive bagasse had the highest nitrogen (6.63 %), protein (41.46 %) and antioxidant capacity (54.4 %). Mushrooms produced in substrate prepared from rice straw and horse manure had the most ash (9.83 %). Mushrooms grown in short method produced compost had the greatest biological efficiency (42.41 %). Based on this experiment rice straw and chicken manure were the better economically substrate for button mushroom compost preparation in the northern provinces of Iran especially Guilan.


  1. Atikpo, M., Onokpise, O., Abazinge, M., Louime, C., Dzomeku, M., Boateng, L. & Awumbilla, B. (2008). Sustainable mushroom production in Africa. A Case Study in Ghana. Africa Journal of Biotechnology, 7, 249-253.
  2. Azizi, A. (1997). Using the waste for mushroom farming and animal feed. Ministry of Agriculture, The Research, Education and Agricultural Extension. (in Farsi)
  3. Baysal, E., Nuri Yigitbasi, O., Colak, M., Toker, H., Simsek, H. & Yilmaz, F. (2007). Cultivation of Agaricus bisporus on some compost formulas and locally available casing materials. Part I: Wheat straw based compost formulas and locally available casing materials. African Journal of Biotechnology, 6(19), 2225-2230.
  4. Bernas, E., Jaworska, G. & Lisiewska, Z. (2006). Edible mushrooms as a source of valuable nutritive constituents. Acta Scientiarum Polonorum Technology Alimentry, 5, 5-20.
  5. Board, N. (2006). Mushroom cultivation and processing (with dehydration, preservation and canning). Asia Pacific Business Press Inc. P: 544.
  6. Bonati, M., Karnopp, P., Soares, H.  M. & Furlan, S. A. (2004). Evaluation of Pleurorus ostreatus and  Pleurotus sajor-caju nutritional characteristics wheat cultivated in different Lignocelluloses waste. Food Chemistry, 88, 425-428.
  7. D’ Angelo, S., Amelia, C., Raimo, M., Salvatore, A., Zappia, V. & Galletti, P. (2007). Effect of Reddening–Ripening on the Antioxidant Activity of Polyphenol Extracts from Cv. ‘Annurca’ Apple Fruits. Journal of Agricultural and Food Chemistry, 55(24), 9977-9985.
  8. Ebadi, A., Alikhani, H. A., Yakhchali, M. B. & Aryanfar, H. (2013). Evaluation of different organic wastes for cultivation for bottom mushroom and their effect on yield and nutrient intake. Journal of Soil Management & Sustainable Producation, 5(1), 243-252.
  9. Farsi, M. & Pourianfar, H. R. (2011). Cultivation and Breeding of the White Button Mushroom. Press Mashhad University. (in Farsi)
  10. Guebel, D. V., Nudel, B. C., Giulietti, A. M. (1991). A simple and rapid micro-kjeldahl method for total nitrogen analysis. Biotechnology Techniques, 5(6), 427-430.
  11. Jesus, J., Kohori, C., Andrade, M. & Miuhoni M. (2013). Yield of different white button strains in sugar cane by product-based composts. African Journal of Agricultural Research, 8(9), 824-831.
  12. Kalberer, P. P. (1991).  Water relations of the mushroom culture (Agaricus bisporus):  Influence on the crop yield and on dry matter contentof the fruitbodies. Mushroom Sciences, 13, 269-274.
  13. Kariaga, M. G. (2005). Important factors in composting for production of high yields in button mushrooms and Agaricus bitorquis (Quel) Saccardo. African Crop Science Conference Proceedings, 7, 1273-1277.
  14. Kirbag, S. & Akyuz, M. (2009). Evaluation of agricultural wastes for the cultivation of Pleurotus eryngii (DC. ex Fr.) Quel. var. ferulae Lanzi. African Journalof Biotechnology, 7(20), 3660-3664.
  15. Lambert, J. D., Hong,  J., Yang, G. Y., Liao, J. & Yang, C. S. (2005). Inhibition of carcinogenesis by polyphenols: evidence from laboratory investigations. The American Journal of Clinical Nutrition, 81, 284-291.
  16. Rasouli, F., Peyvast, Gh., Olfati, J. A. & Ehteshami, S. M. R. (2014). Using vermicompost in casing soil for button mushroom (Agaricus bisporus) cultivation. Iranian Journal of Horticultural Science, 45(4), 377-382.
  17. Seaby, D. (1999). The influence on yield of mushrooms (Agaricus bisporus) of the casing layer pore space volume and ease of water uptake. Compost Science & Utilization, 4, 56-65.
  18. Tajeddin, B. (1994). The effect of substratesenrichment on mushroom P. Sajor-cajuanddetermine some of the properties of its quality and quantity. Master Thesis Tarbiat Modarres University in Tehran. (in Farsi)
  19. Uddin, M. J., Haque, S., Haque, M. E., Bilkis, S. & Blswas, A. K. (2012). Effect of different substrate on growth and yield of button mushroom. Journal of Environmental Science & Natural Resources, 5(2), 177-180.
  20. Kalmis, E. & Sargin, S. (2004). Cultivation of tow pleurotusspecies on wheat straw substrates containing olive mill waste water. International Biodeterioration & Biodegradation, 53, 43-47.
  21. Manzi, P., Gambelli, L., Marconi, S. & Pizzoferrato, L. (1999). Nutrients in edible mushroom: An interspecies comparative study. Food Chemistry, 65, 477-482.
  22. Mollaie, F. & Besharati, H. (2011). Effect of plant growth promoting bacteria (PGPR) on the qualitative and quantitative properties of button mushroom (Agaricus bisporus) in various substrates of industrial and agricultural waste. Journal of Soil (Soil Science and Water), 25(4), 384-373. (in Farsi)
  23. Niknahad, A. (1997). The effect of different formulations using of agricultural waste and  industry alterant. Master Thesis Tehran University. (in Farsi)
  24. Polonia, I. (2004). Olive mill wastes as substrate to produce Pleurotus mushrooms. International Biodeterioration & Biodegradation, 57, 37-44.
  25. Vieyra, F. E., Palazzi, V. I., Pinto, M. S. & Borsarelli, C. D. (2009). Combined UV–Vis absorbance and fluorescence properties of extracted humic substances-like for characterization of composting evolution of domestic solid wastes. Geoderma, 151, 61-67.
  26. Wang, D., Sakoda, A. & Suzuki, M. (2000). Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent Beer grain. Bioresouree Technology, 78, 293-300.