Study of sodium nitroprusside application on micropropagation of Gerbera jamesonii cv. bayoder

Document Type : Full Paper

Authors

1 M. Sc. Student, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Assistant Professor, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Associate Professor, Faculty of Agriculture, Guilan University, Guilan, Iran

4 M. Sc. Student, Faculty of Agriculture, University of Kurdistan, Iran

Abstract

Nitric oxide (NO) has been found as an important growth regulator and posses much attention in different aspects of science, including medicine, biochemistry, physiology and genetics. NO enhances hormonal effects through cytokinen signal transduction in vitro. The stimulation effect of sodium nitroprusside (SNP) is by releasing NO during in vitro culture. In this experiment, the effect of NO on Gerbera micropropagation has been studied based on completely randomized design. Leaf explants were cultured on modified MS, containing 0.5 mg/L 2,4-D and different concentrations of SNP (0, 20, 40, 60, 80, μM). In the second experiment, proliferation of shoot was studied on MS containing 0.5 mg/l BAP+0.1mg IAA and different concentrations of SNP (0, 20, 40 and 60 μM). Then, the regenerated shoots were transferred to modified MS with 0.1mg IAA and different levels of SNP (0, 10, 20 and 30 μM). The results showed that the maximum amount of leaf callus formation and stimulation were observed in 60 μM treatment. There was a significant difference between different concentration of NO and control. The highest proliferation of shoots and plant weight were obtained in medium containing 20 μM SNP compared with the medium without SNP. Sodium nitroprusside increased root diameter along with root weight.

Keywords

References

  1. Agampodi, V. A. & Jayawardena, B. (2009). Effect of coconut (Cocos nucifera L.) water extracts on adventitious root development in vegetative propagation of Dracaena purplecompacta L. Acta Physiologiae Plantarum, 31, 279-284.
  2. Askari, N., Fotouhi, R. & Moini, A. (2005). In vitro plantlet production of two cultivares Gerbera from young capitulums. Iranian Journal of Horticultural science and Technology, 6(4), 203-214. (in Farsi)
  3. Beligni, M. V. & Lamattina, L. (2000(. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta, 210, 215-22.
  4. Constantinovici, D. & Sandu, C. (1995(. Studies on the regenerative capacity of different Gerbera explants for effective in vitro multiplication. Cercetari Agronomice in Molecular Dova, 28, 149-152.
  5. Han, X., Yang, H., Duan, K., Zhang, X., Zhao, H., You, S. & Jiang, Q. (2009(. Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets. Plant Cell, Tissue & Organ Culture, 96, 29-34.
  6. Kalra, C. & Babbar, S. B. (2010). Nitric oxide promotes in vitro organogenesis in Linum usitatissimum L. Plant Cell, Tissue & Organ Cultuer, 103, 353-359.
  7. Kopyra, M. & Gwózdz, W. A. (2004(. The role of nitric oxide in plant growth regulation and responses to abiotic stresses. Acta Physiologiae Plantarum, 26, 459-472.
  8. Laskowski, M. J., Williams, M. E., Nusbaum, H. C. & Sussex, I. M. (1995). Formation of lateral root meristems is a two-stage process. Development, 121, 3303-3310.
  9. Leshem, Y. A. Y., Wills, R. B. & Ku, V. V. (1998(. Evidence for the function of the free radical gas nitric oxide (NO) as an endogenous maturation and senescence regulating factor in higher plants. Plant Physiology and Biochemistry, 36- 825-833.
  10. Manai, J., Kalai, T., Gouia, H. & Corpas, F. (2014(. Exogenous nitric oxide (NO) ameliorates salinity-induced oxidative stress in tomato (Solanum lycopersicum) plants. Journal of soil science and plant nutrition, 14, 433-446
  11. Tan, C. B., Chin, C. F. & Alderson, P. (2013(. Effects of sodium nitroprusside on shoot multiplication and regeneration of Vanilla planifolia Andrews. In vitro Cellular & Developmental Biology-Plant, 49, 626-630.
  12. Thomas, T. D. (2008(. The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26, 618-631.
  13. Tun, N. N., Holk, A. & Scherer, G. F. (2001(. Rapid increase of NO release in plant cell cultures induced by cytokinin. FEBS letters, 509, 174-176.
  14. Van son, N. G. U. Y. E. N. (2007). Response of Gerbera (Gerbera jamesonii bolus) varieties to micropropogatio. Ph. D. thesis, University of Agricultural Sciences, UAS, Dharwad.
  15. Xu, J., Yin, H., Wang, W., Mi, Q. & Liu, X. (2009(. Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita. Plant Growth Regulation , 59, 279-285.
  16. Zottini, M., Formentin, E., Scattolin, M., Carimi, F., Lo Schiavo, F. & Terzi, M. (2002(. Nitric oxide affects plant mitochondrial functionality in vivo. FEBS letters, 515, 75-78.

Files

History

  • Receive Date: 30 December 2014
  • Revise Date: 30 April 2015
  • Accept Date: 11 May 2015

Share

How to cite

Statistics