Document Type : Full Paper
M.Sc. Student, Department of Plant production, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran
Associate Professor, Department of Plant production, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran
Associate Professor, Department of Food Science, Agricultural Sciences and Natural Resources, University of Gorgan, Gorgan, Iran
Salicylic acid (SA) and Methyl jasmonate (MeJA) as phytohormone regulate synthesis of secondary metabolites in a wide range of plant species by accumulation of phenylalanine ammonia lyase enzyme (PAL) and accelerate the production of oxygen free radicals, protein kinase, proline and nitrite oxide content. To study the effect of these compounds on synthesis of secondary metabolites, in two separate experiments the callus of artichoke was treated with different levels of MJ and SA (0, 50, 100, 200,250 µM) in the solid MS medium. A positive correlation between proline accumulation and phenolic compounds and negative correlation by photosynthesis pigments showed that in artichoke, the cells try to balance primary and secondary metabolites. Based on the obtained results, when the elicitors applied to cell culture, the amount of phenyl propanoid compounds and proline significantly increased. Application of SA at 100µM had the highest effect on the production of chlorogenic acid and caffeic acid as well as proline. And MJ in 100 µM had the maximum amount of caffeic acid and proline and highest amount of chlorogenic acid was seen in 200 µM. It can be concluded that poly phenolic compounds production, influenced by executed treatments. Optimization the elicitor concentrations could lead to desirable secondary metabolite production of artichoke under in-vitro conditions.