Importin genes expression in high and low rooting olive genotypes (Olea europaea L.)

Document Type : Full Paper

Authors

Research Assistant and Senior Researcher of Institute of Biosciences and Bioresources (IBBR-CNR), Perugia, Italy

Abstract

One of the fundamental processes in woody plant propagation especially olive is rooting ability of cuttings. Thus the selection of superior trees with desirable genetic structure is essential. Adventitious root formation is a key step in rooting ability. Therefore, identification of inducible genes in these processes is necessary. In this study, for the first time importin genes were identified based on incomplete data of the first linkage map of olive and rooting traits. Generally, importin gene family are contributed to export and import of macromolecules through nuclear pores. Results of qRT-PCR in cross progenies during different time courses showed that importins expression were increased in low rooting than the high rooting progenies, which importin2 gene expression was more tangible. It is suggested that importin genes probably import the rooting inhibitor protein into the cell. Therefore, to select high- and low-rooting olive genotypes in the early stages of development, requires further complementary experiments.

Keywords


  1. Al-Salem, M.M. & Nabila, S.K. (2001). Auxin, wounding and propagation medium affect rooting response of stem cuttings of Arbutus andrachne. HortScience, 36 (5), 976-978.
  2. Bassil, N.V., Proebsting, W.M., Moore, M.W. & Lightfoot, D.A. (1991). Propagation of Hazelnut stem cuttings using Agrobacterium rhizogenes. HortScience, 26 (8), 1058-1060.
  3. Clarke, P.R. & Zhang, C. (2008). Spatial and temporal coordination of mitosis by Ran GTPase. Nature Reviews Molecular Cell Biology, 9, 464-477.
  4. Hedayati, V., Mousavi, A., Razavi, K., Cultrera, N., Alagna, F., Mariotti, R., Hosseini Mazinani, M. & Baldoni, L. (2015). Polymorphisms in AOX2 gene are associated with rooting ability of olive cuttings. Plant Cell Report, 34(7), 1151-64.
  5. Henrique, A., Campinhos, E.N., Ono, E.O. & Pinho, S.Z.D. (2006). Effect of plant growth regulators in the rooting of Pinus cuttings. Brazilian Archives of Biology and Technology, 49(2), 189-196.
  6. Hosseini-Mazinani, M. Mariotti, R. Torkzaban, B. Sheikh-Hassani, M. Ataei, S. Cultrera, N. Pandolfi, S. & Baldoni, L. (2014). High genetic diversity detected in olives beyond the boundaries of the Mediterranean sea. PLOS ONE, 9(4), 1-16.
  7. Johnson, L. (1957). A review of the family Oleaceae. Contributions from the New South Wales national herbarium, 2, 397-418.
  8. Joseph, J. (2006). Ran at a glance. Journal of the Cell Science, 119, 3481-3484.
  9. La Rosa, R.,  Angiolillo, A., Guerrero, C., Pellegrini, M., Rallo, L., Besnard, G., Bervill, A., Martin, A. & Baldoni, L. (2003) A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers. Theatrical and Applied Genetics, 106, 1273-1282.
  10. Livak, K.J. & Schmittgen, T.D. (2001) Analysis of relative gene expression data using Real-Time Quantitative PCR and the 2-DDCT method. Methods, 25, 402-408.
  11. Meier, I. & Brkljacic, J. (2009). Adding pieces to the puzzling plant nuclear envelope. Current Opinion Plant Biology, 12, 752-759.
  12. Meier, I. & Brkljacic, J. (2009). The nuclear pore and plant development. Current Opinion in Plant Biology, 12, 87-95.
  13. Mousavi, S., Hosseini-Mazinani, M., Arzani, K., Yadollahi, A., Pandolfi, S., Baldoni, L. & Mariotti, R. (2014). Molecular and morphological characterization of Golestan (Iran) olive ecotypes provides evidence for the presence of promising genotypes. Genetic Resources and Crop Evolution, 61, 775-785.
  14. Plafker, S. & Macara, I. (2000). Importin-11, a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2. The EMBO Journal, 20, 5502-5513.
  15. Santos Macedo, E., Cardoso, H.G. & Hernandez, A. (2009). Physiologic responses and gene diversity indicate olive alternative oxidase as a potential source for markers involved in efficient adventitious root induction. Plant Physiology, 137, 532-552.
  16. Santos Macedo, E., Sircar, D., Cardoso, H.G., Peixe, A. & Arnholdt-Schmitt, B. (2012). Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism. Plant Cell Reoport, 31, 1581-1590.
  17. Sebastiani, L. & Tognetti, R. (2004). Growing season and hydrogen peroxide effects on root induction and development in Olea europaea L. (cvs ‘Frantoio’ and ‘Gentile di Larino’) cuttings. Scientia Horticulturae, 100, 75-82.
  18. Xu, X.M., Meulia, T. & Meier, I. (2007). Anchorage of plant RanGAP to the nuclear envelope involves novel nuclear-pore associated proteins. Current Biology, 17, 1157-1163.
  19. Zazimalova, E. & Napier, R. M. (2003). Points of regulation for auxin action. Plant Cell Report, 21, 625-634.
  20. Zhao, Q., Brkljacic, J. & Meier, I. (2008). Two distinct, interacting classes of nuclear envelope-associated coiled-coil proteins are required for the tissue-specific nuclear envelope targeting of Arabidopsis RanGAP. Plant Cell, 20, 1639-1651.