Effect of arbuscular mycorrhiza fungi symbiosis and culture media on establishment and growth of micropropagated MM106 apple rootstock

Document Type : Full Paper




Tissue culture is one of the common methods for propagation of apple clones. However, low survival and poor growth of these plants, after transplanting, limit the widespread use of this technique. The success rate of this method can be increased by using bioagents such as arbuscular mycorrhizal fungi (AMF). In this study, effect of mycorrhizal symbiosis and culture media on growth and establishment were studied in acclimatization stage of micropropagated MM106 plants. This study was carried out in greenhouse conditions as a factorial experiment based on a randomized complete design (RCD) with two factors including four levels of mycorrhiza species (Glomus intraradices, Glomus mosseae, Glomus intradices + Glomus mosseae and control) and three levels of substrates (peat moss, perlite, peat moss + perlit) with four replications. After 15 weeks, some characteristics were measured. Results showed that symbiosis with mycorrhizal fungi specially Glomus intraradices could increase plant height, leaf number, roots dry and fresh weight, leaf chlorophyll, Phosphorus, Iron and Zinc levels. Height and fresh weight of roots were increased (1.63 and 1.91 times, respectively) in inoculated plants with Glomus intraradices compared to control. Also, the heightest amount of measured characteristics (except leaf area) was obtained in inoculated plants with AMF and peat moss substrate.

Main Subjects

  1. Aka-Kacar,Y., Akpinar, C., Aslihanagar, A., Ortas, I., Serce, S. & Yalcin-Mendil, Y. (2010). The effect of mycorrhiza in nutrient uptake and biomass of cherry rootstock during acclimatization. Romanian Biotechnological Letters, 15(3), 5246-5252.
  2. Allen, M.F., Moore, T.S. J.r. & Christensen, M. (1982). Phytohormone changes in Bouteloua gracilis infected by vesicular-arbuscular mycorrhizae: II. Altered levels of gibberellin-like substances and abscisic acid in the host plant. Canadian Journal of Botany, 60,468-471.
  3. Alberto de Lima Morais,T., Franklin de Melo, N., Mayumi Yano‑Melo, A. & Ricardo Gonçalves de Oliveira, J. (2011). Acclimatization of Tapeinochilos ananassae plantlets in association with arbuscular mycorrhizal fungi. Agropecuaria brasileira. Brasilia, 46(9), 1099-1104.
  4. Anisha, P.N. (2009). Studies on indusing variaility in vitro and Use of mycorrhizae in hardening of Gerbera. M.Sc. thesis. University of Agricultural Sciences, Dharwad, India.
  5. Awotoye, O.O., Adewole, M.B., Salami, A.O. & Ohiembor, M.O. (2009). Arbuscular mycorrhiza contribution to the growth performance and heavy metal uptake of Helianthusannuus LINN inpot culture. African Journal of environmental Scince and Technology, 3, 157-163.
  6. Baby, U.I. & Manibhushanrao, K. (1996). Influence of organic amendments on arbuscular mycorrhizal fungi in relation to rice sheath blight disease, Mycorrhiza, 6, 201-206.
  7. Barin, M., Aliaasgharzaded, N. & Samadi, A. (2007). Effect of NaCL salinity and salt mixture on Prolin concentration and some tomato growth characteristics in symbiosis with Arbuscular mycorrhiza fungis.Iranian journal of Agricultural Science, 37, 139-147. (in Farsi)
  8. Calvet, C., Pinochet, J., Hernandez-Dorrego, A., Estan, V. & Camprubi, A. (2001). Field microplot performance of the peach-almond hybrid GF-677 after inoculation with arbuscular mycorrhizal fungi in a replant soil infested with root-knot nematodes. Mycorrhiza, 10, 295-300.
  9. Clark, R.B. & Zeto, S.K. (2000). Mineral acquisition by arbuscular mycorrhizal plants. Journal of Plant Nutrition, 23, 867-902.
  10. Cantliffe, D., Shaw, J.N., Jovicich, E., Rodriguez, J.C., Secker, I. & Karchi, Z. (2001). Passive ventilated high-roof greenhouse production of vegetables in a humid mild winter climate. Acta Horticulturae, 559, 515-520.
  11. Cui, M. & Caldwell, M.M. (1996). Facilitation of plant phosphate acquisition by arbuscular mycorrhiza from enriched soil Patches Roots and hyphae exploiting the Same soil Volume. New phytologist, 133, 453-460.
  12. Demir, S. (2004). Influence of arbuscular mycorrhiza on some physiological growth parameters of pepper. Turkey Journal of Biology, 28, 85-90.
  13. Egamberdigevaa, D. & Haflich, G. (2003). Influence of growth-promoting bacteria on the growth of wheat in different soils and temperatures. Soil Biology and Biochemistry, 35, 973-978.
  14. Fascella, G. & Zizzo, G.V. (2005). Effect of Growing Media on Yield and Quality of Soilless Cultivated Rose. Acta Horticulturae, 697, 133-138.
  15. Emami, A. (1996). Methods of plant analysis. Soil & Water Research (Institute.928). (in Farsi)
  16. Giovannetti, M. & Mosse, B. (1980). An evaluation of technique to measure vesicular - arbuscular mycorrhizal infection in roots. New Phytologist, 84, 489-500.
  17. Gholami, A. & Kuchaki, A. (2001). Mycorhiza in Sustainable Agriculture. (212 pp). Shahrud University Press.
  18. Hazarika, B. N. (2003). Acclimatization of tissue-cultured plants. Current Sciences, 85, 12-25.
  19. Jeffries, P. & Barea, J.M.  (2001). Arbuscular mycorrhiza: a key component of sustainable plant- soil ecosystems, In: Hock, B. & Vol, I.X. (Eds), The Mycota: fungal associations. (pp. 95-113.) New York, Springer.
  20. Kapoor, R., Sharma, D. & Bhatnagar, A. K. (2008). Arbuscular mycorrhizae in micropropagation systems and their potential applications. Scientia Horticulturae, 116,227-239.
  21. Karimi, A., Khodaverdiloo, H., Sepehri, M. & Rasouli Sadaghiani, M.H. (2011). Arbuscular mycorrhizal fungi and heavy metal contaminated soils. African Journal of Microbiology Reserch, 5, 1571-1576.
  22. Koch, B.L., Covey, R.P. & Larsen, H.J. (1981). Response of apple seedlings in fumigated soil to phosphorous and vesicular–arbuscular mycorrhiza. Scientia Horticulturae, 17, 232-233.
  23. Krishna, H., Singh, S.K., Sharma, R.R., KHawale, R.N., Grover, M. & Patel, V.B. (2005). Biochemical changes in micro propagated grape (Vitis vinifera L.) plantlets due to arbuscular-mycorrhizal fungi (AMF) inoculation during ex vitro acclimatization. Scientia Horticulturae, 106, 554-564.
  24. Linderman, R.G. (1992). Vesicular-arbuscular mycorrhizae & soil microbial interactions. In:Bethlenfalvay, G.J. & Linderman, R.G. (Eds), Mycorrhizae in Sustainable Agricultura. (pp. 45-70.) Special Publication.
  25. Linderman, R.G. (1994). Role of VAM in biocontrol. In: Pfleger, F.L., Linderman, R.G. (Eds), Mycorrhizae and Plant Health. (pp. 1-26). APS Press, St Paul.
  26. Lieten, F., Jean-Marie, K. & Georges, B. (1995). Effect of prolonged cold storage on the production capacity of strawberry plants. Scientia Horticulturae, 60(3-4), 213-219.
  27. Makekzadeh, P., Khara, J. & Farshian, S. (2007). Effect of arbuscular mycorrhiza (Glomus etunicatum) on some physiological growth parameters of tomato plant under copper toxicity in solution. Pakistan Journal of Biology Sciences, 10, 1326-1330.
  28. Marschner, H. & Dell, B. (1994). Nutrient uptake in mycorrhizal symbiosis. In: A.D. Robson et al. (Eds), Management of Mycorrhizas in Agriculture, Horticulture and Forestry. (pp. 89-102.) Kluwer Academic Publishers, Dordrecht, The Netherlands.
  29. Meenakshisundaram, M. & Santhaguru, K. (2011). Studies on associatioin of arbuscular mycorrhizal fungi with Gloconacetobacter diazotrophicus and its effect on improvement of Sorghum bicolor(L). Journal of Current research in Science, 1, 23-30.
  30. Mereti, M., Grigoriadou, K. & Nanos, G. (2002). Micropropagation of the strawberry tree Arbutus unedo L. Scientia Horticulturae, 93, 143-148.
  31. Mohammadi Ghehsareh1, A., Samadi, N. & Borji, H. (2011). Comparison of date-palm wastes and perlite as growth substrates on some tomato growing indexes. African Journal of Biotechnology, 10(24), 4871-4878.
  32. Morin, F., Fortin, J.A., Hamel, C., Granger, R.L. & Smith, D.L. (1994) Apple rootstock response to vesicular–arbuscular mycorrhizal fungi in a high phosphorous soil. Journal of the American Society for Horticultural Science, 119, 578-583.
  33. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Journal of Plant Physiology, 15, 473-497.
  34. Ortas, I., Ortakci, D., Kaya, Z., Cinar, A. & Onelge, N. (2002b). Mycorrhizal dependency of sour orange (Citrus aurantium L.) In term of phosphorus and zinc nutrition by different levels of phosphorus and zinc application. Journal of Plant Nutrition, 25, 1263-1279.
  35. Phillips, J.M. & Haymann, D.S. (1970) Improved procedures for clearing and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Transaction of the British Mycological Society, 55, 158-161.
  36. Preece, J.E. & Sutter, E.G. (1991). Acclimatization of micropropagated plants to the greenhouse and the field. In: Debergh P.C., Zimmermann, R.H. (Eds.), Micropropagation. (pp. 71-93.) Kluwer Academic Publishers, Dordrecht.
  37. Raiesi, F. & Ghollarata, M. (2006). Interactions between phosphorus availability and an AM fungus (Glomus intraradices) and their effects on soil microbial respiration, biomass and enzyme activities in a calcareous soil. Pedobiologia, 50, 413-425.
  38. Rupanwar, B. S. & Naval, A. M. (2000). Effect of VA-mycorrhizal inoculation on growth of pomegranate Layers. Journal of Maharashtra Agricultur, 25(1), 44-46.
  39. Ruzic, D.J.V. & Vujovic, T.I. (2008). The effect of cytokinin types and their Concentration  on in vitro ,ultiplication of sweet cherry cv. Scientia  Horticulturae, 35, 12-21.
  40. Saif, S.R. (1981). The influence of soil aeration on the efficiency of vesicular-arbuscular mycorrhizas: I. Effect of soil oxygen on the growth and mineral uptake of Eupatorium odoratum L. inoculated with Glomus macrocarpus. New Phytology, 88, 649-659.
  41. Smith, S.E. & Read, D.J. (1997). Mycorrhizal Symbiosis. Mycorrhizal Symbiosis, 2nd edn. Academic Press, San Diego, CA.
  42. Salisbury, F.B. & Ross, C.W. (1995).  Plant Physiology. (4th ed). Wadswordth Pub. Co. Belmont, California, USA.
  43. Syvertsen, J.P. & Graham, J.H.(1984). Influence of vesicular– arbuscular mycorrhiza on the hydraulic conductivity of roots of two Citrus rootstocks. New Phytologist, 97, 277-284.
  44. Taiz, L. & Zeiger, E. (2002). Plant Physiology. (2nd ed). Sinauer Assoc. Inc. Pub. Sunderland, Massachusetts, USA.
  45. Tehranifar, A., Poostchi, M., Arooei, H. & Nematti, H. (2007). Effects of seven substrates on qualitative and quantitative characteristics of three strawberry cultivars under soilless culture. Acta Horticulturae, 761, 485-488.
  46. Wada, S. (2005). Nursery Container Weeds Respons to Modification of Substrate pH, Substrate Particle Size and Applied Nitrogen Form. Master of Science Thesis. Oregan State University.
  47. Wallander, H. (2000) up takes of P from apatite by Pinus sylvestris seeding colonization by different ectomycorrhizal fungi. Plant and Soil, 218, 249-256.
  48. Wang, C., Knill, E., Glik, G. & Defago, G. (2000). Effect of transferrnse 1-aminocyclopropan-1-carboxylic acid (Acc) deaminase genes derivative CHA96 on their growth-promoting and disease suppressive capacities. Canadian Journal of Microbiology, 46,888-907.
  49. Wu, Q.S. & Xia, R.X. (2006). Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under wellwatered and water stress conditions. Journal of Plant Physiology, 163, 417-425.