Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Cryopreservation of Populus euphratica Oliv. Seeds and Evaluation of the Cryopreserved Seeds under Laboratory and Greenhouse Conditions

Document Type : Full Paper

Authors
Maryam Jebeli 1
Mohabat Ali Naderi Shahab 1
Hamid Reza Feizi 2
Alh Ashraf Jafari 1
1 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
2 B.Sc., Agriculture and Natural Resources Research Center, Amlash, Gilan
10.22059/ijhs.2015.54627
Abstract
Populus euphratica Oliv. is a highly heat, saline and cold tolerant native species, with wide range of ecological habitats and conditions. Seed longevity of P. euphratica is too short to be stored in traditional gene banks. For evaluating preservation of P. euphratica seeds in normal conditions (+22oC) as well as in cryogenic conditions (-196oC), three pre-cryopreservation treatments including PVS2, Desiccation and 30% Glycerol were applied before transferring the seeds into liquid nitrogen (-196oC) for 1 week, 1 month and 1 year. To evaluate the effects of the pre-treatments on seed germination and establishment, a part of the seeds were treated with PVS2, Desiccation and 30%Glycerol and incubated at +22oC, instead of liquid nitrogen (-196oC). The 30%Glycerol and PVS2 pre-treatments dramatically reduced the seed germination from 90% in control seeds to 35.00 and 41.67%. Cryogenic (-196oC) condition reduced seed germination from 90% in control seeds to 26.67% and 28.33. However, Desication showed excellent protection of seeds in cryogenic conditions in seed germination and establishment in lab and greenhouse. Control seed in normal conditions stayed for few months. Germination of cryopreserved seeds for a period of one year did not show significant reduction, compared to those kept for one week or one month at -196 oC.
Keywords
seed
Populus euphratica
Cryopreservation
desiccation
liquid nitrogen

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Iranian Journal of Horticultural Science
Volume 46, Issue 2 - Serial Number 2
Summer 2015
Pages 313-322

  • Receive Date 18 March 2014
  • Revise Date 30 June 2014
  • Accept Date 12 July 2014