Hamid Reza Roosta1*, Samira vaziri Nasab2 and Mahmoud Raghami3

Document Type : Full Paper

Authors

Associate Professor, Former Graduate Student and Assistant Professor, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan

Abstract

In order to find the most effective concentration of 6-Banzilamino purine (BAP) and cycocel (CCC) on microtuber production of two potato cultivars under tissue culture conditions, a factorial experiment was carried out with randomized compelete design with 4 replications. Four levels of BAP (0, 5, 10 and 15 mg L-1) and four levels of CCC (0, 250, 500 and 1000 mg L-1) were applied on two potato cultivares (Sante and Arinda). Results showed that the best cultivar for microtuber production was Arinda and the best plant growth regulator combination regarding to microtuber number was 10 mg L-1 BAP+250 mg L-1 CCC. Although, the highest microtuber yield achived at 5 mg L-1 BAP without CCC. Tuber dry weight and tuber diameter were reached to the highest levels at 10 mg L-1 BAP without CCC. On the other hand, higher microtuber number, yield, and the larger microtuber, acompanied with decrease of shoot and root length were obtained with BAP and CCC application.
 

Keywords

References

  1.  

    1. Akita, M. & S. Takayama. (1994). Stimulation of potato (Solanum tuberosum L.) tuberization by semicontinuous liquid mediumsurface level control. Plant Cell Report, 3, 184-187.
    2. Al-Safadi, B., Ayyoubi Z. & Jawdat. D. (2000). The action on effect of gamma irradiation on potato microtuber production in vitro. Plant Cell, Tissue and Organ Plant Cell, Culture, 61, 183-187.
    3. Aphalo, P., Rikala, R. & Sa´nchez, R.A. (1997). Effect of CCC on th morphology and growth potential of containerised silver birch seedlings. New Forestery, 14, 167-177.
    4. Aryakia, Y. & Hamidoghli, E. (2010). Comparison of Kinetin and 6- Banzyl Amino Purine Effect on in vitro Microtuberization of Two Cultivars of Potato (Solanum tuberosum L.). American-Eurasian Journal Agriculture & Environment Science, 8(6), 710-714.
    5. Aslam, A., & Iqbal, J. (2010). Combined effect of cytokinin and sucrose on in vitrotuberization parameters of two cultivars i.e., diamant and red norland of potato (solanum tuberosum). Pakistan Journal of Botany, 42(2), 1093-1102.
    6. Dashtban, A. & laei, Gh. (1997) Investigation of the methods of potato storing into the soil. 5th congress of Horticultural Sciences, Shiraz University, Shiraz, pp 415. (In Farsi)
      1. Davies, H.V. (1988) Rapid determination of glucose, fructose and sucrose in potato tubers by capillary gas chromatography. Potato Research 31: 569-572.
    7. Donnelly, D., Coleman, K. & Ecoleman, S. (2003). Potato microtuber production and performance. American Journal of Potato, 80, 103-115.
    8. Ebadi, M. & Iranbakhsh, A. (1999). Induction and growth of potato microtubers cv Sante in response to the different concentration of banzylaminopurine and sucrose in tissue culture condition. Journal of Basic Sciences of Islamic Azad University, 78(1), 1-14. (In Farsi)
    9. FAO. (2000). FAO Yearbook Production, Food and Agriculture Organization of United Nation, Rome.
    10. Gopal, J., Minocha, L. & Dhaliwal, H.S. (1998). Microtuberization in potato (Solanum tuberosum L.). Plant Cell Report, 17, 794-798.
    11. Hussain, I., Chaudhery, Z., Muhamad, A., Asghar, R. & Rashid, H. (2006). Effect of chlorocholine chloride, sucrose and BAP on in vitro tubrization in potato (Solanum tuberosum L. CV. Cardinal). Pakistan Journal of Botany, 38(2), 275-282.
    12. Kawakami, J., Kazuto, I., Toshihiro, H. & Yutaka, J. (2003). Growth and yield of potato plants grown from microtubers in fields. American Journal of Potato Research, 80, 371-378.
    13. Kefi, S., Pavlista, A.D. Read P.E. & Kachman, S.D. (2000). Comparsion of thidiazuron and two nitroguanidines to kinetin on potato micrituberization in vitro under short and long days. Plant Growth Regulators, 19, 429- 436.
    14. Leclerc Y., Doimelly, D. & Seabrook, J.E.A. (1994). Microtuberization of layered shoots and nodal cuttings of potato: The influence of growth regulators and incubation periods. Plant, Cell, Tissue and Organ Culture, 37, 113-120.
    15. Liu, J. & Xie, C. (2001). Correlation of cell division and cell expansion to potato microtuber growth in vitro. Plant Cell, Tissue and Organ Culture, 67, 159-164.
    16. Naik., P. & Karihaloo, J. (2007). Micropropagation for Production of Quality Potato Seed in Asia-Pacific. Asia-Pacific Consortium on Agricultural Biotechnology, New Delhi, India. 54 P.
    17. Peng, M., Wang, X. & Li, L. (2010). The Effect of Plant Growth Regulator and Active Charcoal on the Development of Microtubers of Potatoes.American Journal of Plant Sciences, 3, 1535-1540
    18. Piao, X.C., Chakrabarty, D., Hahn, E.J. & Paek, K.Y. (2003). A simple method for mass production of potato microtubers using a bioreactor system. Current Scientists, 84, 1129-1132.
    19. Rolot, J. L., Seutine, H. & Michelant, D. (2002). Production de minituber cules depomme de terre par hydroponic. Biotechnology Agronomy Society and Enviroment, 6(3), 155-161.
    20. Sharma, S., Kaure, N. & Gupta, A. (1998).Effect of chlorocholine chloride sprays on the carbohydrate composition and activities of sucrose metabolising enzymes in potato (Solanum tuberosum L.). Plant Growth Regulation, 26, 97-103.
    21. Shibli, R.A., Abu-Ein, A.M. & Ajlouni, M.M. (2001). In vitro and in vivo multiplication of virus free ‘Spunta’ potato. Pakistan Botany, 33, 35-41.
    22. Struik, P.C. & Wiersema, S.G. (1999). Seed Potao technology. wageningen pers,wageninhen, Plant Cell,Tissus and Organ Culture, 65, 173-174.
    23. Tovar, P., Estrada, R., Schilde-Rentschler, L. & Dodds, J. H. (1985). Induction and use of in vitro potato tubers. CIP Circular, 13(4), 1-5.
    24. Vreugdenhil, D. (2007). Potato biology and biotechnology advances and perspectives. Elsevier. 2td,oxford, UK.
    25. Wang, H.Q. & Xiao, L.T. (2009). Effects of chlorocholine chloride on phytohormones and photosynthetic characteristics in potato (Solanum tuberosum L.). Journal of Plant Growth Regulators, 28, 21–27.
    26. Wang, H., Xiao, L., Tang, J. & Liu, F. (2010). Foliar application of chlorocholine chloride improves leaf mineral nutrition, antioxidant enzyme activity, and tuber yield of potato (Solanum tuberosum L.). Scientia Horticulturae, 125, 521-523.
    27. Zhang, Z., Zhou, W. & Li, H. (2005). The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato. ACTA Physiologiae Plantarum, 27 (3B), 363-369.
    1. Hussey, G., & Stacey, N.J. (1984). Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Annual Botany, 53,565–578.

    19.    Nistor, A., Campeanu, G., Atanaslu, N., Chiro, N. & Karcsony, D. (2010). Influence of Genotype over Microtubers Production. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 209 - 212.

    1. Sanchez, G.E., Slack, S.A., & Dodds, J.H. (1991). Response of selected Solanum species to virus eradication therapy. American Potato Journal 68, 299–315.

     

     

Files

History

  • Receive Date: 03 February 2014
  • Revise Date: 09 July 2014
  • Accept Date: 09 August 2014

Share

How to cite

Statistics