Effect of foliar application of putrescine to enhance drought tolerance of Persian walnut (Juglans regia L.) seedlings

Document Type : Full Paper

Authors

Former Graduate Student and Assistant Professor, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

 
To study the effect of foliar application of putrescine on enhanceing drought tolerance of Persian walnut (Juglans regia L.) seedlings, an experiment had been carried out as factorial based on completely randomized design in a controlled greenhouse with 9 treatments and 20 replications. In this study, treatments were putrescine (0, 0.5 and 1 mM) and three stress levels including control (80% of field capacity), moderate drought stress (50% field capacity) and severe drought stress (20% field capacity). The results of morphological, physiological and biochemical parameters showed that these parameters were affected by the stress levels as well as different concentrations of putrescine. Foliar spray of 1mM putrescine affected the physiological and biochemical parameters and enhanced the tolerance of Persian walnut seedlings to drought stress through improving antioxidant activity systems (via increasing catalase, ascorbate peroxidase and guaiacol peroxidase). The highest rate of shoot length (35.67 cm), leaf area (303.6 cm), leaf relative water content (58.51%), the total fresh weight (47.20 mg) and the lowest ion leakage (28.28%) was related to 1 mM putrescine treatment. It was found that foliar spray of 1mM putrescine was effective on enhanceing drought tolerance of Persian walnut (Juglans regia L.) seedlings.

Keywords

References

  1.  

    1. Alexieva, V., Sergiev, I., Mapelli, S. & Karanov, E. (2001). The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environmental, 24, 1337-1344.
    2. Amri, E. & Shahsavar, A. R. (2010). Response of lime seedlings (citrus aurantifolia l.) to exogenous spermidine treatments under drought stress. Australian Journal of Basic and Applied Sciences, 4(9), 4483-4489.
    3. Amri, E., Mirzaei, M., Moradi, M. & Zare, K. (2011). The effect of spermidin and putrescine polyamine on growth of pomegranate (Punicagranatum) in salinity circumstance. International Journal of Plant Physiology and Biochemistry, 3,43-49.
    4. Atkinson, D. (1980). The distribution and effectiveness of the roots of tree crops. Horticulture Review, 2, 424-490.
    5. Bates, L. S., Waldran, R. P. & Tear, I. D. (1973). Rapid determination of free proline for water studies. Plant and Siol, 39, 205-208.
    6. Bouchereau, A., Aziz, A., Larher, F. & Martin-Tanguy, J. (1999). Polyamines and environmental challenges: recent development. Plant Sciences, 140,103-125.
    7. Bradford, M. M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
    8. Deshmukh, P. S., Sairam, R. K. & Shukla, D. S. (1991). Measurement of ion leakage as a screening technique for drought resistance in wheat genotypes. Indian journal of plant physiology, 34, 89-91.
    9. Dhindsa, R.S., Plumb-Dhindsa, P. & Thorpe, T.A. (1981). Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32, 93-101.
    10. F. A. O. (2011). Production Year Book. Vol. 62. F.A.O. Rome, Italy.
    11. Farahani, H., Valadabadi, A., Daneshian, J. & Khalvati, M. (2009). Medicinal and aromatic plants farming under drought conditions. Journal of Horticulture and Forestry, 1(6), 86-92.
    12. Fernandez, J. A., Balenzategui, L., Ba˜nَn, S. & Franco, J. A. (2006). Induction of drought tolerance by paclobutrazol and irrigation deficit in (Phillyrea angustifolia) during the nursery period. Scientia Horticulturae, 107, 277-283.
    13. Fu, J. & Huang, B. (2001). Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany, 45, 105-114.
    14. Fulton, A. & Buchner, R. (2006). The effect of water stress on walnut tree growth, productivity, and economics. University of California, 15pp.
    15. Galston, A. W., Kaur-Sawhney, R., Altabella, T. & Tiburcio, A. F. (1997). Plant polyamines in reproductive activity and response to a biotic stress. Acta Botanica, 110, 197-207.
    16. Goyal, M. & Asthir, B. (2010). Polyamine catabolism influences antioxidative defense mechanism in shoots and roots of five wheat genotypes under high temperature stress.Plant Growth Regulators, 60, 13-25.
    17. Gupta, S., Agarwal, V. P. & Gupta, N. K. (2012). Efficacy of putrescine and benzyladenine on photosynthesis and productivity in relation to drought tolerance in wheat (Triticum aestivum L.). Physiology and Molecular Biology of Plants, 18(4), 331-6.
    18. Hajiboland, R. & Ebrahimi, N. (2011). Growth, photosynthesis and phenolics metabolism in tobacco plants under salinity and application of polyamines. Journal of Plant Biology, 8, 13-26.
    19. Hussein, M. M., Nadia, EL-Gereadly, H. M. & EL-Desuki, M. (2006). Role of putrescine in resistance to salinity of pea plants (Pisum sativum L.). Applied Science Research, 2, 598-604.
      1. Kafi, M., Borzoi. A., Salehi, M., Kamandi, A., Masoumi. & Nabatei, J (2010). Environmental Stress Physiology of Plants. Press Jahad Daneshgahi. 502 PP.
    20. Kamiab, F., Talaie, A.R., Khezri, M. & Javanshah, A. (2013). Exogenous application of free polyamines enhance salt tolerance of pistachio (Pistacia vera L.) seedlings. Plant Growth Regulators, 72, 257-268.
    21. Kaur-Sawhney, R., Tiburcio, A. F. & Galston, A. W. (2003). Polyamines in plants: An overview. Journal of Cell and Molecular Biology, 2, 1-12.
    22. Kusano, T., Berberich, T., Tateda, C. & Takahashi, Y. (2008). Polyamines: essential factors for growth and survival. Planta, 228, 367-381.
    23. Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids pigments of photosynthetic biomemberanes. Methods in Enzymology, 148, 350-382.
    24. Liu, J. H., Kitashiba, H., Wang, J., Ban, Y. & Moriguchi, T. (2007). Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology, 24, 117-126.
    25. Lotfi, N., Vahdati, K., Kholdebarin, B. & Najafian Ashrafi, E. (2009). Germination, mineral composition and ion uptake in walnut under salinity conditions. HortScience, 44(5),1352-1357.
    26. Lotfi, N., Vahdati, K., Kholdebarin, B. & Amiri, R. (2010a). Soluble sugars and proline accumulation play a role as effective indices for drought tolerance screening in Persian walnut (Juglans regia L.) during germination. Fruits, 65, 97-112.
    27. Lotfi, N., Vahdati, K., Kholdebarin, B., Hassani, D. & Amiri, R. (2010b). Peroxidase, guaiacol peroxidase and ascorbate peroxidase activity accumulation in leaves and roots of walnut trees in response to drought stress. Acta Horticulturae, 861, 309-316.
    28. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts, Plant Cell Physiology, 22, 867-880.
    29. Nasibi, F., Manouchehri Kalantari, Kh. & Yaghoobi, M. M. (2011). Comparison the effects of sodium nitroprusside and arginine pretreatment on some physiological responses of tomato plant (Lycopersicun esculentum) under water stress. Iranian Journal ofBiology, 24(6) , 833-847 (In Farsi).
    30. Natali, S., Begnami, C. & Fusari, A. (1991). Water consumption, photosynthesis transpiration and leaf water potential in Olea europaea L. cv. ‘Frantoio’ at different levels of available water. Acquires Global Agri-Med Technologies, 121(3), 205-212.
    31. Noohpishe, Z.  & Kalantari Kh. M. (2011). The interaction effects of spermidine application and salinity stress in pepper plants. Iranian Journal ofBiology, 24(6), 848-857.
    32. Pan, Y., Wu, L. & Yu, Z. (2006). Effect of salt and drought stress on antioxidant enzymes activites and SOD isoenzymes of liquorice (Glycyrriza uralensis Fisch). Plant Growth Regulators, 301, 564-571.
    33. Rostami Shahraji, T., Hajimerzai, A. & Shabaian, N. (2010). Physiological responses of Pistacia khinjuck (stocks) seedling to water stress. Indian Journal of Biology Technologly, 1,44-49.
    34. Sadrzadeh, M. & Moalemi, N. (2006). Effects of drought stress and potasium in vegitative intimacy olive seedling cultivars ‘Zard and Baghmalek’. Plant, soil and water Agriculture research, 6(4),139-148 (In Farsi).
    35. Saxena, N. P., Krishnamurthy, L. & Johansen, C. (1993). Registration of a drought resistance chickpea gerplasm. Crop Sciences, 33, 1424.
    36. Sharma, P. & Dubey, R. S. (2010). Protein synthesis by plants under stressful conditions. In: Pessarakli, M. (Ed.) Handbook of plant and crop stress. CRC Press, Boca Raton, 465–518 pp.
    37. Singh, D. B., Verma, S. & Mishra, S. N. (2002). Putrescine effect on nitrate reductase activity, organic nitrogen/protein and growth in heavy metal and salinity stressed mustard seedlings. Plant Biology, 45, 605-608.
    38. Somogy, M. (1952). Note on sugar determitation. Journal of Biochemistry, 195, 19-29.
    39. Spann, T. M. & Heerema, R. J. (2010). A simple method for non-destructive estimation of total shoot leaf area in tree fruit crops. Scientia Horticulturae, 125, 528-533.
    40.  Vahdati, K. & Leslie, C. A. (Eds.) (2013). Abiotic Stress - Plant Responses and Applications in Agriculture. 410 pp.
    41. Vahdati, K., Lotfi, N., Kholdebarin, B., Hasani, D. & Amiri, R. (2009). Screening for drought tolerant genotypes of Persian walnuts (Juglans regia L.) during seed germination. HortScience, 44(7), 1815-1819.
    42. Verma, S. & Mishra, S. N. (2005). Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology, 62, 669-677.
    43. Wheutherley, P. E. (1950). Studies in water relations of cotton plants. The field measurement of water deficit in leaves. New phytologist, 49, 81-87.
    44. Zhang, K. & John, P.C.L. (2005). Raised level of cyclin dependent kinase after prolonged suspension culture of Nicotiana plumbaginifolia is associated with more rapid growth and division, diminished cytoskeleton and lost capacity for regeneration: implications for instability of cultured plant cells. Plant Cell Tissue and Organ Culture, 82, 295-308.
    45. Zhao, H. & Yang, H. (2008). Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus Hupehensis rehd. Scientia Horticulturae, 116, 442-447.

     

     

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History

  • Receive Date: 05 January 2014
  • Revise Date: 14 June 2014
  • Accept Date: 30 June 2014

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