The effect of Mycohrizal symbiosis on water uptake efficiency and some growth traits of osteospermum (Osteospermum hybrida ‘Passion Mix’)

Document Type : Full Paper


1 MSc. Student, Dep. of Horticulture Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Asist. Prof. Dep. of Horticulture Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Asist. Prof. Soil and Water Research Institute, Karaj, Iran


To study the effect of different arbuscular mycorrhiza fungi on growth performance and water absorption efficiency of osteospermum, a factorial experiment based on a randomized complete block design was conducted in greenhouse. A total of 22 different species of mycorrhizal fungi in symbiosis with the plant studied in this experiment. The results showed that mycorrhiza fungus Glomus mossea CA and Glomus mossea st6 had a significant effect on growth indices of osteospermum compared to the others. Inoculation of osteospermum plants with the fungus Glomus mossea CA improved the water use efficiency better than other species of mycorrhizal fungi. Despite the low rates of colonization in inoculated plants with fungus Glomus sp St2 and its insignificant impact on the characteristics of this plant, high water absorption efficiency was observed. Fungus Glomus mossea St2 and Glomus sp St2 had no effective symbiotic relationship with this plant. Furthermore, plants inoculated with the fungus Glomus mossea st6 had significantly higher root length, root fresh weight and root surface compared to the plants inoculated with other strains. Thus, it was shown that different species of arbuscular mycorrhiza fungi depending on the species used, can lead to improve some growth traits in this plant.


  1. Aliabadi Farahani, M.H., Lebaschi, H., Shiranirad, A.M., Valadabadi, A.R. & Daneshian, J. (2008). Effects of arbuscular mycorrhizal fungi, different levels of phosphorus and drought stress on water use efficiency, relative water content and proline accumulation rate of Coriander (Coriandrum sativum L.). Journal of Medicinal Plants Research, 2 (6), 125-131.
  2. Al-Karaki, G.N. (1998). Benefit, cost and water-use efficiency of arbuscular mycorrhizal durum wheat grown under drought stress. Mycorrhiza, 8 (1), 41-45.
  3. Al-Karaki, G.N. (2000). Growth of mycorrhizal tomato and mineral acquisition under salt stress. Mycorrhiza, 10 (2), 51-54.
  4. Aroca, R., Porcel, R. & Ruiz-Lozano, J.M. (2007). How does arbuscular mycorrhizal symbiosis regulate root hydraulic properties and plasma membrane aquaporins in Phaseolus vulgaris under drought, cold or salinity stresses? New Phytologist, 173 (4), 808-816.
  5. Auge, R.M. (2001). Water relations, drought and VA mycorrhizal symbiosis. Mycorrhiza, 11, 3-42.
  6. Bago, B., Pfeffer, P.E. & Shachar-Hill, Y. (2000). Carbon metabolism and transport in arbuscular mycorrhizas. Plant physiology, 124 (3), 949-958.
  7. Bethlenfalvay, G.J. (1992). Mycorrhizae and crop produtivity. In: G.J. Bethlenfalvay and R.G. Linderman (eds.) Mycorrhizae in sustainable agriculture. ASA Spacial Publication, Madison, pp. 1-28.
  8. Bolandnazar, S., Aliasgarzad, N., Neishabury, M.R. & Chaparzadeh, N. (2007). Mycorrhizal colonization improves onion (Allium cepa L.) Yield and water use efficiency under water deficit condition. Scientia horticulturae, 114 (1), 11-15.
  9. Fitter, A. (1986). Effect of benomyl on leaf phosphorus concentration in alpine grasslands: a test of mycorrhizal benefit. New Phytologist, 103 (4), 767-776.
  10. Giovannetti, M. & Mosse, B. (1980). An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist, 84 (3), 489-500.
  11. Giovannini, A., Zottini M., Morreale, G., Spena, A. & Allavena, A. (1999). Ornamental traits modification by rol genes in Osteospermum ecklonis transformed with Agrobacterium tumefaciens. In vitro Cellular & Developmental Biology-Plant, 35, 70-75.
  12. Henderson, J.C. & Davies, F.T. (1990). Drought acclimation and the morphology of mycorrhizal Rosa hybrida L. cv.‘Ferdy’ is independent of leaf elemental content. New Phytologist, 115 (3), 503-510.
  13. Khalvati, M., Bartha, B., Arthur Dupigny, A. & Schröder, P. (2010). Arbuscular mycorrhizal association is beneficial for growth and detoxification of xenobiotics of barley under drought stress. Journal of Soils and Sediments, 10 (1), 54-64.
  14. Khandan-Mirkohi, A. & Schenk, M.K. (2009). Phosphorus efficiency of ornamental plants in peat-substrates. Journal of Plant Nutrition and Soil Science, 172, 369-377.
  15. Koltai, H. (2010). Mycorrhiza in floriculture: difficulties and opportunities. Symbiosis, 52 (2-3), 55-63.
  16. Kormanik, P.P., Bryan, W.C. & Schultz, R.C. (1981). Effects of three vesicular-arbuscular mycorrhizal fungi on sweetgum seedlings from nine mother trees. Forest science, 27 (2), 327-335.
  17. Koske, R. & Gemma, J. (1989). A modified procedure for staining roots to detect VA mycorrhizas. Mycological research, 92 (4), 486-488.
  18. Long, L.K., Yao, Q., Huang, Y.H., Yang, R.H., Guo, J. & Zhu, H.H. (2010). Effects of arbuscular mycorrhizal fungi on zinnia and the different colonization between Gigaspora and Glomus. World Journal of Microbiology and Biotechnology, 26 (8), 1527-1531.
  19. Marulanda, A., Azcón, R. & Ruiz-Lozano, J.M. (2003). Contribution of six arbuscular mycorrhizal fungal isolates to water uptake by Lactuca sativa plants under drought stress. Physiologia Plantarum, 119 (4), 526-533.
  20. Miller, M.H. (2000). Arbuscular mycorrhizae and the phosphorus nutrition of maize: a review of Guelph studies. Canadian Journal of Plant Science, 80 (1), 47-52.
  21. Nagahashi, G., Douds J. & Abney, G.D. (1996). Phosphorus amendment inhibits hyphal branching of the VAM fungus Gigaspora margarita directly and indirectly through its effect on root exudation. Mycorrhiza, 6 (5), 403-408.
  22. Nagarathna, T.K., Prasad, T.G., Bagyaraj, D.J. & Shadakshari, Y.G. (2007). Effect of arbuscular mycorrhiza and phosphorus levels on growth and water use efficiency in Sunflower at different soil moisture status. Journal of Agricultural Technology, 3, 221-229.
  23. Nowak, J. (2001). The effects of rooting media, CO2 enrichment, P-nutrition and mycorrhizal inoculation on rooting and growth of Osteospermum. International Symposium on Growing Media and Hydroponics, 644.
  24. Nowak, J. & Nowak, J.S. (2013). CO2 Enrichment and mycorrizal effects on cutting growth and some physiological traits of cuttings during rooting. Acta Scientiarum Polonorum-Hortorum Cultus, 12 (6), 67-75.
  25. Rillig, M.C. & Mummey, D.L. (2006). Mycorrhizas and soil structure. New Phytologist, 171 (1), 41-53.
  26. Ruiz-Sanchez, M., Aroca, R., Muñoz, Y., Polón, R. & Ruiz-Lozano, J.M. (2010). The arbuscular mycorrhizal symbiosis enhances the photosynthetic efficiency and the antioxidative response of rice plants subjected to drought stress. Journal of plant physiology, 167 (11), 862-869.
  27. Safir, G.R., Boyer, J.S. & Gerdemann J.W. (1972). Mycorrhizal enhancement of water transport in soybean. Plant Physiology, 49 (5), 700-703.
  28. Secilia, J. & Bagyaraj, D. (1992). Selection of efficient vesicular-arbuscular mycorrhizal fungi for wetland rice (Oryza sativa L.). Biology and fertility of soils, 13 (2), 108-111.
  29. Secilia, J. & Bagyaraj, D. (1994). Selection of efficient vesicular-arbuscular mycorrhizal fungi for wetland rice-a preliminary screen. Mycorrhiza, 4 (6), 265-268.
  30. Sharma, A.K. (2002). Biofertilizers for sustainable agriculture, Agrobios (India). pp. 218.
  31. Sheikh-asadi, M., Taheri, M.R., Khandan-Mirkohi, A. & Babalar, M. (2014). Evaluation of the symbiosis possibility of mycorrhizal fungus with some ornamental plants and its effect on yield of these plants. M.Sc. Thesis. Faculty of agricultural sciences and engineering department of horticultural sciences, Tehran. (In farsi).
  32. Sylvia, D.M. & Burks, J.N. (1988). Selection of a vesicular-arbuscular mycorrhizal fungus for practical inoculation of Uniola paniculata. Mycologia, 80 (4), 565-568.
  33. Tennant, D. (1975). A test of a modified line intersect method of estimating root length. The Journal of Ecology, 63, 995-1001.
  34. Thomas, R.S., Dakessian, S., Ames, R.N., Brown, M.S. & Bethlenfalvay, G.J. (1986). Aggregation of a silty clay loam soil by mycorrhizal onion roots. Soil Science Society of America Journal. 50 (6), 1494-1499.
  35. Treseder, K.K. (2004). A meta‐analysis of mycorrhizal responses to nitrogen, phosphorus, and atmospheric CO2 in field studies. New Phytologist, 164 (2), 347-355.
  36. Troeh, Z.I. & Loynachan, T.E. (2003). Endomycorrhizal fungal survival in continuous corn, soybean, and fallow. Agronomy Journal, 95 (1), 224-230.
  37. Vasanthakrishna, M., Bagyaraj, J.D. & Nirmalnath, J.P. (1995). Selection of efficient VA mycorrhizal fungi for Casuarina equisetifolia-second screening. New Forests, 9 (2), 157-162.
  38. Wu, Q.S. & Xia, R.X. (2006). Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. Journal of plant physiology, 163 (4), 417-425.
  39. Wu, Q.S., Xia, R.X. & Zou, Y.N. (2008). Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress. European Journal of Soil Biology, 44 (1), 122-128.
  40. Wu, Q. & Zou, Y. (2009). Mycorrhiza has a direct effect on reactive oxygen metabolism of drought-stressed citrus. Plant Soil and Environment, 55 (10), 436-442.