Micropropagation of Fig (cv. Jaami-e-Kan) Via Meristem Tip Culture



A methodology to regenerate whole plants of fig tree (Ficus carica cv. Jaami-e-Kan) from apical meristem of greenhouse plantlets was developed. Two meristematic explants size (0.2-0.4 and 0.5-0.7 mm), two kinds of culture media and three concentrations of 6-benzyladenine (0.5, 1 and 1.5 mgrl-1) were tested as a completely randomize design (CRD) with a simple factorial arrangement for establishment stage of meristem explants. The survival in percentage of meristems was recorded at the end of 5th week. Best developed meristems were taken for proliferation stage. MS medium supplemented with BA (0.5, 1, 1.5, 2 mgl-1) and NAA (0, 0.1, 0.5 mgl-1) through a completely randomized design with a simple factorial arrangement were employed for shoot regeneration. Data were started to be recorded following 6 weeks. Four concentrations of IBA were added to ½ MS and the experiment conducted under a completely randomized design of a simple factorial arrangement and applied for rooting stage. Percentage of rooting plants, the number of roots per plant and length of roots were assessed after 5 weeks in culture. A high percentage of survival was obtained in BA 0.5 mgl-1 treatment. Meristems of larger sizes showed the most survival. The proliferation rate was found to be higher in the medium containing 2 mgl-1 BA than in the other concentrations while the shortest shoots obtained in this concentration. The highest number of rooted plants were obtained from 1.5 and 2mgl-1 IBA. Also, 2 mgl-1 of IBA treatment was shown to generate the greatest number of roots per plant.