In Vitro Propagation of Alstroemeria cv. ‘Fuego’

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Abstract

Alstroemeria (Alstroemeriaceae), commonly called the Peruvian Lily or Lily of the Incas, is one of the most important cut flowers throughout the world, commonly propagated through rhizome splitting. Because of such impediments as low multiplication rate, time consuming process and high risk of carrying and transfer of viral diseases, in vitro propagation techniques based on rhizome meristem culture have been on the developmental stages nowadays. In this experiment, lateral and terminal buds of rhizomes (4-6mm) were cultured on solidified MS medium containing 30 g/l of sucrose supplemented with different concentrations of BAP and NAA after surface sterilization and were then subcultured every three weeks. Some growth factors as number of rhizomes, shoots and roots, length of shoots and roots were studied. Explants started to grow after three weeks. The greatest number of shoots was obtained in the medium supplemented with 1.5 mg/l of BAP and 0.2 mg/l of NAA. Furthermore, the results showed that higher concentrations of BAP caused a reducing length of shoots due to a decrease in apical dominance; also it was shown that the presence of a low NAA concentration in the medium was necessary for primordial shoot and rhizome growth. Consequently, the medium containing 0.5 mg/l of BAP and 0.2 mg/l of NAA, producing on the average, 4.1 rhizomes along with 2.62 shoots per explant resulted in the most suitable hormonal treatment for micropropagation of Alstroemeria cv. "Fuego".

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