Document Type : Full Paper
Authors
1
Department of Horticultural Sciences and Engineering, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2
Department of Tissue and Cell Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Abstract
To create inbred lines, expensive and time-consuming methods such as self-pollination are typically used. However, new efficient technologies like doubled haploids and inverse breeding offer effective and faster solutions for producing inbred lines. In this research, doubled haploid lines produced through parthenogenesis embryo induction following pollination with irradiated pollen were utilized. A top-cross test was conducted using 15 doubled haploid lines and the top-cross parent, Beit Alpha, to examine the general combining ability of the lines. Hybrids derived from line × tester (Beit Alpha) crosses were planted in the greenhouse. To evaluate the general combining ability of the lines, several traits were measured and analyzed, including days to flowering, fruit number, fruit weight, average yield per plant, placental diameter, fruit diameter, fruit length, and the length of 20 nodes of the main stem. Comparison of means using the Tukey test revealed that the doubled haploid lines DH34, DH102, DH4, DH36, and DH5, crossed with the common parent Beit Alpha, had the highest fruit yields, with averages of 854.3, 742.7, 617.7, 545.7, and 519.3 grams per plant, respectively. Among these, the top-cross progeny from DH34 × Beit Alpha and DH102 × Beit Alpha had the highest yields, averaging 854.3 and 742.7 grams per plant, respectively. The heritability of some evaluated traits was over 80%, with the number of days to flowering, fruit weight, and fruit length showing the highest heritability.
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