Positive effects of foliar application of silica and potassium nanoparticles on the leaf and fruit characteristics of ‘Zard’ olive cultivar

Document Type : Full Paper

Authors

1 Department of Horticultural Science, Faculty of agriculture, Ilam University, Ilam, Iran

2 Department of Plant Production and Genetic (Plant Biotechnology),, Faculty of Agriculture, Jahrom University, Jahrom, Iran.

3 Department of Horticultural Sciences, Faculty of Agriculture, Ilam University, Ilam, Iran.

4 Department of Chemistry, Masjed Soleiman Azad University, Masjed Soleiman, Iran.

Abstract

Effects of foliar application of silica (Si) and potassium (K) nanoparticles were evaluated on the leaf and fruit attributes of the ‘Zard’ olive cultivar. Potassium nanoparticles (0, 400, and 800 mg L⁻¹) and silica nanoparticles (0, 30, and 60 mg L⁻¹) were sprayed on the trees at two points during the growing season: early May and early July. The study was conducted as a factorial experiment in a completely randomized block design with three replications.
Measured traits included leaf characteristics, fruit fresh and dry weight, pulp-to-stone ratio, fruit dimensions, and Si and K content in both leaves and fruits. According to the results, most of the evaluated traits were significantly affected by foliar application of Si and K. Application of Si and K nanofertilizers increased the leaf area index, with the greatest leaf length observed in trees treated with 400 mg L⁻¹ K and 30 mg L⁻¹ Si.
Both K and Si treatments improved fruit characteristics; however, the effect of K was more pronounced than that of Si. Among all treatments, the combined application of 800 mg L⁻¹ K and 60 mg L⁻¹ Si produced the most favorable fruit traits. Foliar spraying also significantly increased Si and K content in leaves and fruits.
Overall, the findings indicate strong synergistic interactions between Si and K, suggesting that their combined application can be recommended to enhance the yield and performance of olive trees.

Keywords

Main Subjects


Extended Abstract

Introduction

     Olive (Olea europaea L.), a member of the Oleaceae family, is one of the oldest fruit crops domesticated by humans. Although native to Asia Minor and the eastern Mediterranean, its cultivation has expanded widely beyond its natural range due to its remarkable adaptability to diverse climatic conditions and its considerable economic and health-promoting value. Today, the olive is regarded as an important industrial crop worldwide and is cultivated across five continents.

Proper orchard management is a key factor influencing the productivity of tree crops. Foliar fertilization during the growing season is an effective method for supplying essential nutrients to olive trees, particularly under dry conditions and in calcareous soils. The use of agrochemicals to enhance crop productivity has become an integral component of modern agriculture to meet the demands of a growing global population and increasing need for agricultural products. However, the intensive application of synthetic fertilizers has led to soil degradation and nutrient imbalance. Sustainable agriculture therefore relies on strategies that improve nutrient use efficiency and minimize the detrimental environmental effects of agrochemicals.

The use of nanoparticle-based nutrients represents a promising approach to promoting plant performance while supporting sustainable agricultural practices. Due to their extremely small particle size (<100 nm), nanofertilizers possess large surface areas, enabling slow and controlled nutrient release and facilitating efficient uptake through foliar or soil application. These properties provide higher reactivity and improved nutrient availability compared with conventional fertilizers, ultimately enhancing nutrient use efficiency and reducing the negative impacts of synthetic inputs on agricultural ecosystems.

 

Materials and methods

     The effects of foliar application of silica (Si) and potassium (K) nanoparticles were investigated on the leaf and fruit characteristics of the ‘Zard’ olive cultivar. Potassium nanoparticles (0, 400, and 800 mg L⁻¹) and silica nanoparticles (0, 30, and 60 mg L⁻¹) were sprayed on the trees twice during the growing season—once in early May and again in early July. The study was conducted as a factorial experiment in a completely randomized block design with three replications. Measurements included leaf traits, fruit fresh and dry weight, pulp-to-stone ratio, fruit dimensions, and Si and K concentrations in both leaves and fruits.

 

Results and discussion

     The foliar application of silica (Si) and potassium (K) significantly influenced most of the studied traits. Both Si and K nanofertilizers enhanced the leaf area index, with the greatest leaf length observed in trees treated with 400 mg L⁻¹ K and 30 mg L⁻¹ Si.

Our results indicated that while both K and Si treatments improved fruit characteristics, the effect of K was more pronounced than that of Si. The simultaneous application of 800 mg L⁻¹ K and 60 mg L⁻¹ Si yielded the most favorable fruit characteristics. Specifically, the application of Si and K, both individually and in combination, increased the fleshy portion of the fruit. The highest pulp-to-stone ratio (3.13) was recorded with 800 mg/L K and 60 mg/L Si, whereas the control group exhibited the lowest ratio (1.8). The greatest fruit dry weight was achieved with the application of 400 mg/L K and 60 mg/L Si.

Foliar application of both Si and K substantially increased their respective content in olive leaves and fruits. While Si content did not differ significantly between leaves and fruits, K content was significantly higher in the fruit than in the leaves.

The results suggest that Si foliar application positively impacts both vegetative and fruit characteristics of olive trees, partly by enhancing essential nutrient absorption and also through its triggering effects on physiological processes within plant cells, such as antioxidant enzyme activity. Therefore, the inclusion of Si foliar spraying in olive fertilization programs is recommended to improve olive performance.

Overall, this investigation revealed strong synergistic effects between Si and K, supporting the recommendation for their simultaneous application to enhance olive tree yield.

 

Conclusion

     Considering the demand for innovative and environmentally friendly fertilization methods that enhance fruit crop productivity over conventional synthetic chemical fertilizers, this study investigated the effects of potassium (K) and silica (Si) nanoparticles on the vegetative and fruit attributes of the ‘Zard’ olive cultivar.

Both K and Si nanofertilizers significantly supported higher yield in the ‘Zard’ olive cultivar. While individual applications of these nanofertilizers improved olive plant characteristics, the synergistic effects observed when K and Si were applied together proved more beneficial than their separate use, highlighting a strong interaction between these two nutrients.

Our findings demonstrate that applying nutrients in the form of nanofertilizers can enhance olive productivity and optimize nutrient use efficiency. This approach holds the potential to reduce agricultural production costs and minimize nutrient losses from leaching and volatilization.

 

Author Contributions

  1. Erfani-Moghadam, A. Zarei, and A. Shirmardi conceived and planned the experiments. S. Hashemi carried out the experiments. J. Erfani-Moghadam and A. Zarei analyzed data. A. Zarei wrote the first manuscript. J. Erfani-Moghadam, A. Zarei contributed to the interpretation of the results. All authors provided critical feedback and helped shape the research, analysis and manuscript.

 

Data Availability Statement

Data available on request from the authors.

 

Acknowledgements

The authors would like to thank Ilam University for the financial support of this research.

 

Ethical considerations

The authors avoided data fabrication, falsification, plagiarism, and misconduct.

 

Conflict of interest

The author declares no conflict of interest.

REFERENCES
Abdollahi, F.; Erfani-Moghadam, J.; Zarei, A. & Rostaminia, M. (2024). Effect of foliar application of silica and calcium nitrate on cracking, quantitative and qualitative characteristics of pomegranate fruit. Iranian Journal of Horticultural Science, 55(1): 123-134. https://doi.org/10.22059/ijhs.2023.362286.2116 [in Persian]
Abo El-Enien, M.M.S.; Abo El-Kassim, A.B.; El-Azaze, A.M. & El- Sayed, F.S. (2017). Effect of silicon, potassium and calcium compounds on growth and increase the efficiency of citrus seedlings to resist citrus leafminer (Phyllocnistis citrella). Journal of Productivity and Development, 22(3): 729-749.
Al-Hamadani, Z.A.A. & Joody, A. (2021). Effect of sewage and silicon fertilization on the growth of peach trees. Plant Archives, 21: 13195-1398. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.S1.218  
Babu, S.; Singh, R.; Yadav, D.; Rathore, S.S.; Raj, R.; Avasthe, R.; Yadav, S.K; Das, A.; Yadav, V.; Yadav, B.; Shekhawat, K.; Upadhyay, P.K.; Yadav, D,K. & Singh, V.K. (2022). Nanofertilizers for agricultural and environmental sustainability. Chemosphere, 292, 133451. https://doi.org/10.1016/j.chemosphere.2021.133451  
Baghdady, G.A.; Abdrabboh, G.A. & Shahda, M.A. (2020) Effect of some preharvest treatments on yield and fruit quality of Crimson seedless grapvines. Environmental Science, 15: 1-14.
Ben Mimoun, M.; Loumi, O.; Ghrab, M.; Latiri, K. & Hellali, R. (2004). Foliar potassium application on olive tree. IPI regional workshop on Potassium and Fertigation development in West Asia and North Africa; Rabat, Morocco, 24-28 November.
Busso, M.A.; Suñer, L.G. & Rodríguez, R.A. (2022). Review of the effects of different fertilization sources on Olea europaea (Oleaceae). Impact on the yield and quality olives and oil. Considerations on environmental sustainability and soil use. Lilloa, 59 (2): 199-220. https://doi.org/10.30550/j.lil/2022.59.2/2022.08.23   
Bybordi A. (2015). Influence of exogenous application of silicon and potassium on physiological responses, yield, and yield components of salt-stressed wheat. Communications in Soil Science and Plant Analysis, 46: 109-122.
Chen, W.; Yao, X.; Cai, K. & Chen, J. (2011). Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biological Trace Element Research, 142: 67-76.
Debona, D.; Rodrigues, F.A. & Datnoff, L.E. (2017). Silicon's role in abiotic and biotic plant stresses. Annual Review Phytopathology, 4(55): 85-107. https://doir.org:10.1146/annurev-phyto-080516-035312    
Santos Sarah (dos), M.M.; Mello Prado (de), R.; Teixeira G.C.M.; Souza Júnior (de), J.P.; Medeiros (de), R.L.S. & Barreto R.F. (2021). Silicon supplied via roots or leaves relieves potassium deficiency in maize plants. Silicon. https://doi.org/10.1007/s12633-020-00908-1
Erel, R; Kerem, Z.; Ben-Gal, A.; Dag, A.; Schwartz, A.; Zipori, I. & Yermiyahu, U. (2013). Olive (Olea europaea L.) tree nitrogen status is a key factor for olive oil quality. Journal of Agricultural and Food Chemistry, 61(47): 11261-11272. https://doi.org/10.1021/jf4031585
Fageria, N.K.; Dos Santos, A.B. & De Moraes, M. F. (2010). Yield, potassium uptake, and use efficiency in upland rice genotypes. Communications in Soil Science and Plant Analysis, 41(22): 2676-2684.
Fauteux, F.; Rémus-Borel, W.; Menzies, J. G. & Bélanger, R. R. (2005). Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiology Letters, 249(1): 1-6.
Gholami, R.; Fahadi Hoveizeh, N.; Zahedi, S.M.; Padervand, M.; Dawi, E.A. & Carillo, P. (2024) Nanostructure-assisted drought tolerance in olive trees (Olea europaea L.): the role of Fe2O 3-graphitic carbon. Frontier in Plant Science, 15: 1454619. https://doi.org/10.3389/fpls.2024.1454619
Gross-Urrego, J.A.; Camilo Chavez, C.; Pantoja-Benavides, A.D.; Arturo Moreno-Poveda, G.; Ramírez-Godoy, A. & Restrepo-Díaz, H. (2021). Silicon compounds promotes physiological response of avocado 'Hass' and affect the development of pests. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.3988096
Guntzer, F. ; Keller, C. & Meunier, J. D. (2012). Benefits of plant silicon for crops: A review. Agronomy for Sustainable Development, 32: 201–213.
Haberman, A.; Dag, A.; Shtern, N.; Zipori, I.; Erel, R.; Ben-Gal, A. & Yermiyahu, U. (2019). Long-Term impact of potassium fertilization on soil and productivity in intensive olive cultivation. Agronomy. 9(9): 525. https://doi.org/10.3390/agronomy9090525
Hassan, I.F.; Ajaj, R.; Gaballah, M.S.; Ogbaga, C.C.; Kalaji, H.M.; Hatterman-Valenti, H.M. & Alam-Eldein, S.M. (2022). Foliar application of nano-silicon improves the physiological and biochemical characteristics of ‘Kalamata’ olive subjected to deficit irrigation in a semi-arid climate. Plants, 11: 1561. https://doi.org/10.3390/plants11121561
Hodson, M.J.; White, P.; Mead, A. & Broadley, M.R. (2005). Phylogenetic variation in the silicon composition of plants. Annals of Botany, 96(6): 1027–1046. https://doi.org/10.1093/aob/mci255
Kamalizadeh, M.; Bihamta, M. & Zarei, A. (2019). Drought stress and TiO2 nanoparticles affect the composition of different active compounds in the Moldavian dragonhead plant. Acta Physiologiae Plantarum, 41: 21. https://doi.org/10.1007/s11738-019-2814-0
Kamruzzaman, M.; Akter, S.; Khan, M.Z. (2023). Synergistic effects of silicon and phosphorus co-application on rice (Oryza sativa L.) growth, yield and nutrient use efficiency in saline soil. Silicon, 15: 6485–6496. https://doi.org/10.1007/s12633-023-02509-0
Lalithya, K.A.; Bhagya, H. P.; Bharathi, K. & Choudhary, R. (2014). Response of silicon and micro nutrients on fruit character and nutrient content in leaf of sapota. Bioscan, 2(2): 593-598.
Larbi, A.; Kchaou, H.; Gaaliche, B.; Gargouri, K.; Boulal, H. & Morales, F. (2020). Supplementary potassium and calcium improves salt tolerance in olive plants. Scientia Horticulturae, 260: 108912.
Martos-García, I.; Fernández-Escobar, R. & Benlloch-González, M. (2024). Silicon is a non-essential element but promotes growth in olive plants. Scientia Horticulturae, 323(1): 112541. https://doi.org/10.1016/j.scienta.2023.112541
Mengel, K. (2007). Potassium. In Handbook of Plant Nutrition, 1st ed.; Barker, A.V., Pilbeam, D.J., Eds.; 1CRC Taylor and Francis: New York, NY, USA, pp. 91–120.
Nascimento-Silva, K.; Benlloch-Gonzalez, M. & Fernandez-Escobar, R. (2022). Silicon nutrition in young olive plants: effect of dose, application method, and cultivar. HortScience. 57(12): 1534–1539. https://doi.org/10.21273/HORTSCI16750-22
Olyaie Torshiz, A.; Goldansaz, S.H.; Motesharezadeh, B.; Asgari-Sarcheshmeh, M. A. & Zarei, A. (2017). Effect of organic and biological fertilizers on pomegranate trees: yield, cracking, sun burning and infestation to pomegranate fruit moth Ectomyelois ceratoniae (Lepidoptera: Pyralidae). Journal Crop Protection, 6(3): 327–340.
Ol lyaie Torshiz, A.; Goldansaz, S.H.; Motesharezadeh, B.; Asgari-Sarcheshmeh, M. A. & Zarei, A. (2020). The influence of fertilization on pomegranate susceptibility to infestation by Ectomyelois ceratoniae. International Journal of Fruit Science, https://doi.org/10.1080/15538362.2020.1778602
Pasković, I.; Franić, M.; Polić Pasković, M.; Talhaoui, N.; Marcelić, Š.; Lukić, I.; Fredotović, Ž.; Žurga, P.; Major, N.; Goreta Ban, S. (2024). Silicon foliar fertilisation ameliorates olive leaves polyphenolic compounds levels and elevates its potential towards different cancer cells. Applied Sciences. 14(11): 4669. https://doi.org/10.3390/app14114669
Patil, H.; Tank, R.V. & Manoli, P. (2017). Significance of silicon in fruit crops - a review. Plant Archives, 17(2): 769-774.
Pavlovic, J.; Kostic, L.; Bosnic, P.; Kirkby, E.A. & Nikolic, M. (2021). Interactions of Silicon with Essential and Beneficial Elements in Plants. Frontier in Plant Science, 23(12): 697592. https://doi.org/10.3389/fpls.2021.697592
Rawat, J.; Pandey, N. & Saxena, J. (2022). Role of Potassium in Plant Photosynthesis, Transport, Growth and Yield. In: Iqbal, N., Umar, S. (eds) Role of Potassium in Abiotic Stress. Springer, Singapore. https://doi.org/10.1007/978-981-16-4461-0_1
Razeghi-Jahromi, F.; Hosseini-Mazinani, M.; Razavi, K. & Zarei, A. (2021). Analysis of fatty acid compositions and differential gene expression in two Iranian olive cultivars during fruit ripening. Acta Physiologiae Plantarum, 43(43). https://doi.org/10.1007/s11738-021-03218-0
Razeghi-Jahromi, F.; Parvini, F.; Zarei, A. & Hosseini-Mazinani, M. (2022a). Sequence characterization and temporal expression analysis of different SADs and FAD2-2 genes in two Iranian olive cultivars. Scientia Horticulturae, 305: 111415. https://doi.org/10.1016/j.scienta.2022.111415
Razeghi-Jahromi, F.; Parvini, F.; Zarei, A. & Hosseini-Mazinani, M. (2022b). Change in oil composition and the major fatty acids and triacylglycerol biosynthesis genes in drupe of selected olive cultivars during growing season; a two years study. European Journal of Lipid Science and Technology, 124(12): 17548012. https://doi.org/10.1002/ejlt.202200079
Restrepo-Diaz, H.; Benlloch, M. & Fernández-Escobar, R. (2008). Plant water stress and K+ starvation reduce absorption of foliar applied K+ by olive leaves. Scientia Horticulturae, 116(4): 409-413.
Rohi Vishekaii, Z.; Soleimani, A.; Fallahi, E.; Hasani, A. & Ghasemnezhad, M. (2022). Response of olive (Olea europaea L.) trees to foliar spray of nano chelated and chemical potassium fertilizers. Journal of Plant Nutrition, 46(7): 1159–1171. https://doi.org/10.1080/01904167.2022.2072740
Rohi Vishekaii, Z.; Soleimani, A.; Ghasemnezhad, M. & Hasani, A. (2019a). The feasibility for replacement of urea with nitrogen nano-chelated fertilizer in olive (Olea europaea L.) orchards. Iranian Journal of Plant Physiology, 10 (1): 3047-3058.
Rohi Vishekaii, Z.; Soleimani, A.; Ghasemnezhad, M. & Hasani, A. (2019b). The impact of foliar application of boron nano-chelated fertilizer and boric acid on fruit yield, oil content, and quality attributes in olive (Olea europaea L.). Scientia Horiculturae, 257: 108689. https://doi.org/10.1016/j.scienta.2019.108689
Seleiman, M.F.; Almutairi, K.F.; Alotaibi, M.; Shami, A.; Alhammad, B.A. & Battaglia, M.L. (2021). Nano-fertilization as an emerging fertilization technique: why can modern agriculture benefit from its use? Plants, 10: 2. https://doi.org/10.3390/plants10010002
Shang, Y.; Hasan, M.K.; Ahammed, G.J.; Li, M.; Yin, H. & Zhou, J. (2019). Applications of Nanotechnology in Plant Growth and Crop Protection: A Review. Molecules. 13;24(14): 2558. https://doi.org/10.3390/molecules24142558
Singh, S.P. & Endley, N. (2020). Chapter 5 - Fabrication of nano-silica from agricultural residue and their application. In A. Husen & M. Jawaid (Eds.), Nanomaterials for Agriculture and Forestry Applications: 107-134.
Vishekaii, Z.R.; Soleimani, A.; Fallahi, E.; Ghasemnezhad, M. & Hasani, A. (2019). The impact of foliar application of boron nano-chelated fertilizer and boric acid on fruit yield, oil content, and quality attributes in olive (Olea europaea L.). Scientia Horticulturae, 257: 108689.
Yuan, F.; Wu-yan, S.; Vanessa, P. & Fang-qin, C., (2021). Synergistic effect of Si and K in improving the growth, ion distribution and partitioning of Lolium perenne L. under saline-alkali stress. Journal of Integrative Agriculture, 20(6): 1660–1673.
Zarei, A.; Abdollahi, F.; Erfani-Moghadam, J. & Rostaminia, M. (2024). Foliar Application of Silica and Potassium Sulphate on some Characteristics of Pomegranate Fruit cv. ‘Malase-Saveh’. Plant Production, 47(2): 309-321. https://doi.org/10.22055/ppd.2024.46385.2150
Zarei, A.; Erfani-Moghadam, J.; Hashemi, S. & Shirmardi, A. (2024). Effect of foliar application of silicon and potassium nanoparticles on the fatty acid composition of olive oil cv. Zard. Seed and Plant, 39: 597-619. https://doi.org/10.22092/spj.2024.366669.1375
Zhang, Y. ; Liang, Y.; Zhao, X.; Jin, X.; Hou, L. & Shi, Y. (2019). Silicon compensates phosphorus deficit-induced growth inhibition by improving photosynthetic capacity, antioxidant potential, and nutrient homeostasis in tomato. Agronomy, 9: 733. https://doi.org/10.3390/agronomy9110733
Zivdar, S.; Arzani, K.; Souri, M.K.; Moallemi, N. & Seyyednejad, S.M. (2016). Physiological and biochemical response of olive (Olea europaea l.) cultivars to foliar potassium application. Journal of Agricultural Science Technology, 18: 1897-1908.