Document Type : Full Paper
Authors
1
Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University,Tehran, Iran.
2
Department of Molecular Biology, Agricultural Biotechnology Research Institute of North Region, Rasht,, Iran
3
Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
4
Department of Molecular Biology, Agricultural Biotechnology Research Institute of North Region,,, Rasht, Iran
5
Department of Genetics and Breeding, Citrus and Subtropical Fruits Research Center, Horticultural Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ramsar, Iran
6
Department of Post-harvest Physiology and Technology, Citrus and Subtropical Fruits Research Center, Iranian Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ramsar, Iran.
Abstract
The aim of this research was to obtain triploid acid citrus genotypes. Stability of polyploidy level for two genotypes of key lime labeled as A and B and three genotypes of sweet lime labeled as C, D and E were investigated. The results showed that in tree A, four of five main branches (A1, A2, A3 and A4) were diploid (2n=2X) and only one main branch (A5) was mix-ploid (2X+4X). In tree B, all of seven main branches were equally triploid (2n=3X). All branches of C and D trees of sweet lime were tetraploid and those of E tree were diploid. Controlled crossing was made between clementine mandarin and B tree of key lime and between each of three female parents of lemon (ʻEurekaʼ, ʻCook Eurekaʼ and ʻLisbonʼ) and two sweet lime tetraploids (C and D trees). From crossing between clementine mandarin and Mexican lime (B tree) no fruit as well as no seed was obtained. However, from crosses of three female lemons (ʻEurekaʼ, ʻCook Eurekaʼ and ʻLisbonʼ) with tetraploid sweet limes fruits and seeds were obtained. Only very small seeds with high monoembryony were cultured on B5 medium. Fifteen interploid hybrids were analyzed for ploidy level by flowcytometry of which ten seedlings were diploid (nucellar) and five seedlings were triploid (2n=3X), four from ʻEurekaʼ × sweet lime and one from ʻCook Eurekaʼ × sweet lime. The triploid genotypes obtained for the first time here are supposed to be seedless, therefore should be more evaluated for phenotypic characteristics.
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