Document Type : Full Paper
Authors
1
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
2
Department of Plant Breeding and Biotechnology, Agriculture Faculty, University of Zabol, Zabol. Iran
3
Institute of Agricultural Biotechnology, University of Zabol, Zabol. Iran
Abstract
Considering the low amount of important bioactive compounds such as glycyrrhizin and glycyrrhetinic acid in licorice and its complex chemical synthesis, using the hairy root and stimulating their production using elicitors is an efficient method for in vitro production. Therefore, in this research, first, using Agrobacterium rhizogenes, hairy roots were produced. To remove bacteria after inoculation normal cefotaxime and cefotaxime sodium salt, and to increase the production of bioactive compounds, methyl jasmonate elicitor a concentration of 100 micromolar was used. Roots were harvested one, two, three and five days after treatment and traits such as glycyrrhizin, , glycyrrhetinic acid,, TFC, TPC, and antioxidant activity were measured . The results showed that glycyrrhizin, glycyrrhetinic acid and total flavonoids decreased in all treatments compared to the control treatment. The TPC and antioxidant activity were the highest in the treatment that was under the influence of the elicitor for two days and the control treatment, respectively. The lack of effect or the negative effect of MJ on the amount of glycyrrhizin and glycyrrhetinic acid in this research can be attributed to the effect of the species and ecotype, the number of cultures after inoculation, the type of antibiotic, the concentration of the elicitor, and the length of time they have been affected by the elicitor. attributed Considering the large amount of bioactive compounds compared to other researches, the results of this research can provide a suitable solution for increasing glycyrrhizin and glycerethnic acid in a short time and at a lower cost.
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