Effect of light source on in Vitro regeneration of Philodendron'Birkin'

Articles in Press

Document Type : Full Paper

Authors

1 Genetic and Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 2. Master's degree in Agricultural Biotechnology,Department of Agricultural Biotechnology, Sana Institute of Higher Education. Sari, Iran.

Abstract

Light is a key abiotic stimulus that directly or indirectly affects the growth and development of plants. Considering the high importance of light in the growth and regeneration of plants, the use of plant growth lamps in greenhouses and tissue culture laboratories of ornamental plants is inevitable. In the present study, the effect of light source including fluorescent lamp, 100% red LED, 100% blue LED, 30:70 red:blue LED, 70:30 red:blue LED and white full spectrum lamp on direct regeneration of Philodendron Birkin plant was studied. Node explants were cultured on MS culture medium, MS containing IBA (1mg/l) + (1mg/l) BAP and MS culture medium containing (0.5mg/l) TDZ under different light regimes. The highest proliferation of plantlets obtained in the light spectrum of 100% blue LED lamp (47.5 plantlets) and the combination of LED blue (30%): red (70%) with an average proliferation of 44.33 plantlets on MS culture medium containing IBA (1 mg/L) + (1 mg/L) BAP. The light spectrum of 100% blue LED lamps, a combination of blue (70%): red (30%) and fluorescent lamps in MS and MS cultures containing (0.5mg/l) TDZ had the best effect on the growth characteristics of seedlings. The highest content of photosynthetic pigments was observed in the light spectrum of 100% blue and full spectrum white LED lamps in MS culture medium containing (0.5mg/l) TDZ and MS culture medium. The highest content of flavonoid and total phenol was observed under the light spectrum of 100% blue LED lamp on MS culture medium containing (0.5mg/l) TDZ.

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Main Subjects

Iranian Journal of Horticultural Science

Articles in Press, Accepted Manuscript
Available Online from 09 February 2025

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History

  • Receive Date: 08 January 2024
  • Revise Date: 03 September 2024
  • Accept Date: 30 November 2024

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